FastQCFastQC Report
Thu 26 May 2016
SRR1547973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547973_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2227561
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52640.2363122715831351No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.034714E-445.037
CGTAGTC207.034714E-445.016
ACTACGC207.034714E-445.028
TATCGCC207.034714E-445.010
TACCGTA207.034714E-445.024
AGTCGAC207.034714E-445.044
GCGATAC1000.042.7499969
CGACGTC700.041.78571318
CACGACC4650.041.1290327
ACACGAC4800.040.31250426
GTTGACG900.040.01
CGCAATA451.9297659E-840.013
CGGTCTA1650.039.54545631
ATTCGCG800.039.3751
TAGACGG2650.039.0566022
TTACGCG750.039.01
CCGCATG750.039.01
ATACGAG1850.038.918921
TCGATAG1100.038.863641
TTCGATC356.25093E-638.57142635
Produced by FastQC (version 0.11.5)