##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547973_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2227561
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.937497558989406	31.0	31.0	33.0	30.0	33.0
2	31.285780277173107	31.0	31.0	33.0	30.0	34.0
3	31.48726477075151	31.0	31.0	34.0	30.0	34.0
4	35.354490404527645	37.0	35.0	37.0	33.0	37.0
5	35.1781199257843	37.0	35.0	37.0	32.0	37.0
6	35.20495196315611	37.0	35.0	37.0	32.0	37.0
7	35.554515454346706	36.0	35.0	37.0	35.0	37.0
8	35.638426063304216	37.0	35.0	37.0	35.0	37.0
9	37.46449502392976	39.0	37.0	39.0	35.0	39.0
10	36.63616080547289	39.0	37.0	39.0	32.0	39.0
11	36.237745677896136	38.0	35.0	39.0	31.0	39.0
12	35.56757772289962	37.0	35.0	39.0	30.0	39.0
13	35.23369999744115	37.0	35.0	39.0	30.0	39.0
14	36.177905341312766	38.0	35.0	40.0	30.0	41.0
15	36.43187683749177	38.0	35.0	40.0	30.0	41.0
16	36.59690396806193	38.0	35.0	40.0	31.0	41.0
17	36.517156656989414	38.0	35.0	40.0	31.0	41.0
18	36.44969812274501	38.0	35.0	40.0	30.0	41.0
19	36.414205492015704	38.0	35.0	40.0	30.0	41.0
20	36.29902435892889	38.0	34.0	40.0	30.0	41.0
21	36.17058432967717	38.0	34.0	40.0	30.0	41.0
22	36.063381429285215	38.0	34.0	40.0	30.0	41.0
23	36.000110434686185	38.0	34.0	40.0	30.0	41.0
24	35.90444436762899	38.0	34.0	40.0	30.0	41.0
25	35.748838303417955	37.0	34.0	40.0	29.0	41.0
26	35.50748958165455	37.0	34.0	40.0	29.0	41.0
27	35.58968935081912	37.0	34.0	40.0	29.0	41.0
28	35.63802517641492	37.0	34.0	40.0	29.0	41.0
29	35.62466123262169	37.0	34.0	40.0	29.0	41.0
30	35.54796299629954	37.0	34.0	40.0	29.0	41.0
31	35.45383358749772	37.0	34.0	40.0	29.0	41.0
32	35.34568884982274	37.0	34.0	40.0	28.0	41.0
33	35.23708217193603	37.0	34.0	40.0	27.0	41.0
34	35.060443687064016	37.0	34.0	40.0	26.0	41.0
35	34.96090297863897	37.0	33.0	40.0	26.0	41.0
36	34.87424003203504	37.0	33.0	40.0	25.0	41.0
37	34.82054318602274	37.0	33.0	40.0	25.0	41.0
38	34.760249887657395	37.0	33.0	40.0	25.0	41.0
39	34.72522368635472	37.0	33.0	40.0	25.0	41.0
40	34.51514593764211	37.0	33.0	40.0	24.0	41.0
41	34.47435962471959	37.0	33.0	40.0	24.0	41.0
42	34.44143662059086	36.0	33.0	40.0	24.0	41.0
43	34.39435822408455	36.0	33.0	40.0	24.0	41.0
44	34.23724153906448	36.0	33.0	40.0	24.0	41.0
45	34.1176950934228	36.0	33.0	40.0	23.0	41.0
46	34.07608276496132	36.0	33.0	40.0	23.0	41.0
47	34.012446797192084	36.0	33.0	40.0	23.0	41.0
48	33.9370854490629	35.0	33.0	40.0	23.0	41.0
49	33.79482806531448	35.0	33.0	40.0	23.0	41.0
50	33.671321234300656	35.0	32.0	39.0	23.0	41.0
51	33.34212396428201	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	8.0
13	6.0
14	38.0
15	109.0
16	320.0
17	722.0
18	1424.0
19	2616.0
20	4438.0
21	6911.0
22	10315.0
23	14615.0
24	20554.0
25	27976.0
26	35537.0
27	42555.0
28	50076.0
29	59834.0
30	72490.0
31	88195.0
32	108801.0
33	138357.0
34	211567.0
35	239284.0
36	186064.0
37	227788.0
38	307167.0
39	369745.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.34285301277945	23.81793360540968	29.721116503655793	12.118096878155077
2	30.12855764668173	26.540148619948006	29.914511880931656	13.416781852438609
3	29.668368228748843	25.97661747534635	30.089277016431875	14.265737279472932
4	25.946898872803036	29.014873217837806	29.742126029320858	15.296101880038302
5	23.790414718160356	33.26723712616624	28.69779997046097	14.244548185212436
6	22.477139795498307	41.83467927477631	25.461614743659094	10.226566186066286
7	91.04751789064363	3.358471440288279	4.335549060160417	1.2584616089076799
8	91.60880442780243	2.5951253411242163	4.179144813542704	1.6169254175306533
9	86.91241227512961	5.156985599945411	5.598454991804938	2.332147133120036
10	44.73336532647142	33.22934815253095	11.32417922561941	10.713107295378219
11	38.559482770617734	23.695602499774417	22.14035889477325	15.604555834834603
12	35.41245335144582	22.394672918048038	25.093050201543303	17.099823528962844
13	25.198636535654916	30.54856859138762	26.130328193032646	18.122466679924816
14	19.55246118961501	33.38328333096153	27.986079842482432	19.07817563694103
15	19.055145964577402	25.958301478612704	37.92578519735262	17.06076735945727
16	24.023853892216643	23.687297452235875	34.81969741793827	17.469151237609207
17	22.749500462613597	23.210049017737337	27.528000355545817	26.512450164103253
18	24.575488617371196	25.149255171912237	30.460984008967657	19.81427220174891
19	27.904555700158156	27.18843614159163	25.412278272065276	19.494729886184935
20	29.284719924617104	27.102826813721375	25.27567146309349	18.33678179856803
21	26.107747442157585	27.364772502301843	28.38292643837812	18.144553617162448
22	25.417036839844116	24.175948492544087	27.60391297926297	22.803101688348825
23	22.672330858728447	27.55735982089828	27.832369124796134	21.937940195577134
24	22.852797297133502	25.672922088328892	32.21694041150837	19.257340203029234
25	22.12774420094444	26.65834964788843	29.668188660153415	21.545717491013715
26	20.55068301159878	30.39732694188846	26.764384903488615	22.28760514302414
27	20.362270662846047	31.09427755289305	28.171663985857176	20.371787798403727
28	18.83899026783105	29.66145483782487	31.354427555519244	20.14512733882484
29	20.529134780147434	27.283472820721855	31.37512283614231	20.812269562988398
30	22.296314219902396	28.077974071192664	29.878508377548357	19.747203331356584
31	26.601381510988926	26.891519469051577	26.807212013498173	19.699887006461328
32	28.3802329094467	26.312006719456843	26.63338961312395	18.674370757972508
33	27.578099993670207	26.756349208843215	25.768407688947686	19.897143108538888
34	21.714332402120526	27.776209046576056	28.992112898367317	21.517345652936104
35	21.435462373420975	28.2361291116158	29.655439289878032	20.672969225085193
36	27.311126384417754	28.25004567776146	25.68306771397057	18.75576022385021
37	23.195189716465677	30.1444494673771	27.19027671969477	19.470084096462454
38	22.857825217805484	30.78986389149388	24.836536462974525	21.515774427726107
39	22.52266043443928	29.505813757737727	26.52452615214578	21.446999655677217
40	25.233158598125932	25.773884531108237	26.78970407544395	22.20325279532188
41	20.148180004947115	26.18927158448186	27.699578148477187	25.962970262093833
42	22.657516449605644	26.6539951094493	26.336024019095323	24.352464421849724
43	22.438083625992732	25.902635214030052	27.48225525586056	24.177025904116654
44	21.257330326756485	27.72965588821137	28.45304797489272	22.55996581013943
45	21.014239340695944	30.475214820155315	25.522937418997728	22.98760842015101
46	22.616574809848082	29.71698642596095	26.86126216072197	20.805176603468997
47	21.628588397803696	27.37155121677925	28.130183640313327	22.869676745103725
48	23.272898026137106	25.99686383448085	29.649378849782345	21.0808592895997
49	23.294311581141887	24.928834721024476	29.738040843774872	22.03881285405877
50	21.08207137761884	29.272509260128004	27.828912429334146	21.816506932919008
51	20.724191166931007	29.594610428176825	26.81910843294527	22.862089971946897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	962.0
1	1747.0
2	2532.0
3	6643.0
4	10754.0
5	8482.0
6	6210.0
7	6047.5
8	5885.0
9	6468.0
10	7051.0
11	7261.5
12	7472.0
13	7996.5
14	8521.0
15	8382.5
16	8244.0
17	7969.0
18	7694.0
19	7942.0
20	8190.0
21	9221.5
22	10253.0
23	11654.5
24	13056.0
25	14058.0
26	18803.5
27	22547.0
28	27944.0
29	33341.0
30	38705.0
31	44069.0
32	51037.5
33	58006.0
34	62980.5
35	67955.0
36	74438.0
37	80921.0
38	87472.5
39	94024.0
40	110294.5
41	126565.0
42	145264.0
43	163963.0
44	167095.5
45	170228.0
46	167095.5
47	163963.0
48	165047.5
49	166132.0
50	161448.0
51	156764.0
52	149823.5
53	142883.0
54	130270.0
55	117657.0
56	113866.0
57	110075.0
58	104960.0
59	99845.0
60	93917.0
61	87989.0
62	78426.0
63	68863.0
64	57711.5
65	46560.0
66	40273.5
67	33987.0
68	27575.0
69	21163.0
70	17937.0
71	14711.0
72	12458.0
73	10205.0
74	8102.5
75	4797.5
76	3595.0
77	2715.0
78	1835.0
79	1419.0
80	1003.0
81	710.5
82	418.0
83	310.0
84	202.0
85	147.0
86	92.0
87	75.0
88	58.0
89	45.0
90	32.0
91	24.0
92	16.0
93	10.5
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2227561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.648947735800736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5312472196805	14.831740727628691
2	7.060356843882014	2.6333645155531746
3	2.2705371599584456	1.2702938648477549
4	1.1642776539881863	0.8685021247674553
5	0.695474714026172	0.6484935796722524
6	0.47346960505661234	0.5297825951514589
7	0.3925907390859877	0.5124982922341776
8	0.31004591564499007	0.4625624061249523
9	0.2804968904814476	0.4707874665578826
>10	4.428831306706119	21.758784252810226
>50	2.4580609313433497	33.140010619041156
>100	0.9306695925843764	22.123995370293716
>500	0.003448749116695707	0.406795803347006
>1k	2.4633922262112194E-4	0.10056194393298658
>5k	2.4633922262112194E-4	0.24182643803711346
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5264	0.2363122715831351	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.489214885697855E-5	0.0	0.0	0.030885798413601243	0.0
2	4.489214885697855E-5	0.0	0.0	0.12004160604356065	0.0
3	4.489214885697855E-5	0.0	0.0	0.1764710371567827	0.0
4	4.489214885697855E-5	0.0	0.0	0.29615350600948753	0.0
5	4.489214885697855E-5	0.0	0.0	0.4998291853735992	0.0
6	4.489214885697855E-5	0.0	0.0	0.7404511032470042	0.0
7	4.489214885697855E-5	0.0	0.0	0.851828524561168	0.0
8	4.489214885697855E-5	0.0	0.0	1.1186674573670485	0.0
9	4.489214885697855E-5	0.0	0.0	1.212088019138421	0.0
10	4.489214885697855E-5	0.0	0.0	1.4095685819602695	0.0
11	4.489214885697855E-5	0.0	0.0	1.7133088611265865	0.0
12	4.489214885697855E-5	0.0	0.0	1.9348067235869186	0.0
13	4.489214885697855E-5	0.0	0.0	2.039405430423679	0.0
14	4.489214885697855E-5	0.0	0.0	2.087170676807504	0.0
15	4.489214885697855E-5	0.0	0.0	2.155631203814396	0.0
16	4.489214885697855E-5	0.0	0.0	2.2969516884161645	0.0
17	4.489214885697855E-5	0.0	0.0	2.4581593949615748	0.0
18	4.489214885697855E-5	0.0	0.0	2.6461677143746005	0.0
19	4.489214885697855E-5	0.0	0.0	2.7620343505744622	0.0
20	4.489214885697855E-5	0.0	0.0	2.8810434371943123	0.0
21	4.489214885697855E-5	0.0	0.0	3.0361458114951736	0.0
22	4.489214885697855E-5	0.0	0.0	3.196141430021445	0.0
23	4.489214885697855E-5	0.0	0.0	3.376069162640215	0.0
24	4.489214885697855E-5	0.0	0.0	3.5134840302914263	0.0
25	4.489214885697855E-5	0.0	0.0	3.6319095189761357	0.0
26	4.489214885697855E-5	0.0	0.0	3.751187958489128	0.0
27	4.489214885697855E-5	0.0	0.0	3.8849216699340667	0.0
28	4.489214885697855E-5	0.0	0.0	4.007836373504474	0.0
29	4.489214885697855E-5	0.0	0.0	4.1515361419956625	0.0
30	4.489214885697855E-5	0.0	0.0	4.316469896896201	0.0
31	4.489214885697855E-5	0.0	0.0	4.468384928628217	0.0
32	4.489214885697855E-5	0.0	0.0	4.605575335535144	0.0
33	4.489214885697855E-5	0.0	0.0	4.7538092110608865	0.0
34	4.489214885697855E-5	0.0	0.0	4.914612888266584	0.0
35	4.489214885697855E-5	0.0	0.0	5.093777454354785	0.0
36	4.489214885697855E-5	0.0	0.0	5.254940268751338	0.0
37	4.489214885697855E-5	0.0	0.0	5.425754895152142	0.0
38	4.489214885697855E-5	0.0	0.0	5.618117753004295	0.0
39	4.489214885697855E-5	0.0	0.0	5.919882777620905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.034714E-4	45.0	37
CGTAGTC	20	7.034714E-4	45.0	16
ACTACGC	20	7.034714E-4	45.0	28
TATCGCC	20	7.034714E-4	45.0	10
TACCGTA	20	7.034714E-4	45.0	24
AGTCGAC	20	7.034714E-4	45.0	44
GCGATAC	100	0.0	42.749996	9
CGACGTC	70	0.0	41.785713	18
CACGACC	465	0.0	41.12903	27
ACACGAC	480	0.0	40.312504	26
GTTGACG	90	0.0	40.0	1
CGCAATA	45	1.9297659E-8	40.0	13
CGGTCTA	165	0.0	39.545456	31
ATTCGCG	80	0.0	39.375	1
TAGACGG	265	0.0	39.056602	2
TTACGCG	75	0.0	39.0	1
CCGCATG	75	0.0	39.0	1
ATACGAG	185	0.0	38.91892	1
TCGATAG	110	0.0	38.86364	1
TTCGATC	35	6.25093E-6	38.571426	35
>>END_MODULE