FastQCFastQC Report
Thu 26 May 2016
SRR1547972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547972_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2390516
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63490.265591194537079No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG4200.041.252
TTTAGCG1700.041.029411
TACGGGA11200.040.5803574
CGACGGT1000.040.528
CGTTTTT43950.039.675771
TAAGGGA38400.039.316414
ACGGGAT14550.039.278355
GCTACGA1500.039.010
GACCGAT13600.038.5477949
ACGTCGG659.094947E-1238.0769272
TTAGGGA41150.038.0558934
AGGGCGA19450.037.7120826
AAGGGAT50450.037.685835
GGGACCG20100.037.611947
AGGGATG51250.037.580496
GCGTTAG2950.037.3728831
ATAGGGA40050.037.3595474
TAGGGAC33650.037.310555
GGACCGA15350.037.231278
TAGGGAT46550.037.2180445
Produced by FastQC (version 0.11.5)