##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547972_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2390516
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.853639967270663	31.0	31.0	31.0	30.0	33.0
2	31.210030386744954	31.0	31.0	33.0	30.0	34.0
3	31.40572872133046	31.0	31.0	34.0	30.0	34.0
4	35.27210108612534	37.0	35.0	37.0	33.0	37.0
5	35.08435961106305	35.0	35.0	37.0	32.0	37.0
6	35.11367378423738	37.0	35.0	37.0	32.0	37.0
7	35.524055475888886	35.0	35.0	37.0	35.0	37.0
8	35.614969320431236	37.0	35.0	37.0	35.0	37.0
9	37.428556010501495	39.0	37.0	39.0	35.0	39.0
10	36.52470345314568	38.0	35.0	39.0	32.0	39.0
11	36.10497900871611	38.0	35.0	39.0	31.0	39.0
12	35.78504473511158	37.0	35.0	39.0	30.0	39.0
13	35.63836426947153	37.0	35.0	39.0	30.0	39.0
14	36.66155758840351	38.0	35.0	40.0	31.0	41.0
15	36.81449820875493	38.0	35.0	40.0	31.0	41.0
16	36.88315242399549	38.0	35.0	40.0	31.0	41.0
17	36.792693711315884	38.0	35.0	40.0	31.0	41.0
18	36.715925348334835	38.0	35.0	40.0	31.0	41.0
19	36.70265875651951	38.0	35.0	40.0	31.0	41.0
20	36.60863888800577	38.0	35.0	40.0	30.0	41.0
21	36.49145205470283	38.0	35.0	40.0	30.0	41.0
22	36.35981854963531	38.0	35.0	40.0	30.0	41.0
23	36.27665282307251	38.0	34.0	40.0	30.0	41.0
24	36.19522646993369	38.0	34.0	40.0	30.0	41.0
25	36.02940453023531	38.0	34.0	40.0	29.0	41.0
26	35.8019670230193	38.0	34.0	40.0	29.0	41.0
27	35.89274449533072	38.0	34.0	40.0	29.0	41.0
28	35.933385511747254	38.0	34.0	40.0	29.0	41.0
29	35.94046138992586	38.0	34.0	40.0	29.0	41.0
30	35.8605757083408	38.0	34.0	40.0	29.0	41.0
31	35.80083421319916	38.0	34.0	40.0	29.0	41.0
32	35.688922391651005	38.0	34.0	40.0	29.0	41.0
33	35.585936676433036	38.0	34.0	40.0	27.0	41.0
34	35.48025154401811	38.0	34.0	40.0	27.0	41.0
35	35.37438611580094	38.0	34.0	40.0	27.0	41.0
36	35.34208011994063	38.0	34.0	40.0	26.0	41.0
37	35.31571802907824	38.0	34.0	40.0	26.0	41.0
38	35.25990832104868	38.0	34.0	40.0	26.0	41.0
39	35.22954332872066	38.0	34.0	40.0	26.0	41.0
40	35.07686039332094	38.0	34.0	40.0	26.0	41.0
41	35.017138559206465	38.0	34.0	40.0	25.0	41.0
42	34.99421170994045	38.0	33.0	40.0	25.0	41.0
43	34.94970625588785	38.0	33.0	40.0	25.0	41.0
44	34.81991168433928	37.0	33.0	40.0	25.0	41.0
45	34.68504707770205	37.0	33.0	40.0	24.0	41.0
46	34.610441427708494	37.0	33.0	40.0	24.0	41.0
47	34.570429564161046	37.0	33.0	40.0	24.0	41.0
48	34.4820582669181	37.0	33.0	40.0	24.0	41.0
49	34.354953491212775	37.0	33.0	40.0	24.0	41.0
50	34.24586449118098	37.0	33.0	40.0	24.0	41.0
51	33.90001029066528	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	6.0
14	25.0
15	71.0
16	202.0
17	521.0
18	1203.0
19	2090.0
20	3867.0
21	6061.0
22	9398.0
23	13543.0
24	19350.0
25	27087.0
26	35387.0
27	42980.0
28	51431.0
29	61617.0
30	74769.0
31	90989.0
32	110125.0
33	137507.0
34	192425.0
35	220651.0
36	209598.0
37	261670.0
38	364217.0
39	453682.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.80082626512435	24.709142293964987	30.090449091325887	12.399582349584776
2	29.609757893274924	26.634709828338316	30.053134971696487	13.70239730669027
3	28.792486643051124	26.319631410122334	30.753862346037426	14.134019600789118
4	25.267222641471548	29.30174907844164	30.048491622729152	15.38253665735766
5	23.10844185941445	33.63662907924482	28.992317976537286	14.262611084803448
6	22.047164712555784	42.40778978262434	25.098346967767625	10.446698537052251
7	92.32303820597728	2.5901520843198704	3.916936761770262	1.1698729479325802
8	93.10935379641884	1.9794471151835	3.6191349482705824	1.292064140127069
9	88.05270493901735	5.135669453791566	5.022221143886926	1.7894044633041568
10	38.60400850694997	42.049289776767864	10.210306059444907	9.13639565683727
11	27.837086219042252	25.789703980228534	29.039588105664215	17.333621695065
12	28.22629089284489	24.03861760389807	28.810474391302964	18.924617111954074
13	24.3155034310584	27.42006328340827	29.33952334977051	18.924909935762823
14	19.208028726852277	31.195984465278627	29.677316529151028	19.918670278718068
15	19.01535066069418	28.281425432835423	34.84820850393806	17.85501540253234
16	23.14471017972689	27.25298638452953	32.86972352412617	16.732579911617407
17	22.384832396018265	26.60392149644679	28.741577132301146	22.2696689752338
18	22.89560078242522	27.027679379682045	30.874463923270124	19.20225591462262
19	25.10600221876783	27.620312936621218	27.57178784831392	19.701896996297034
20	26.887416775290358	28.396170533893102	26.86060248080331	17.855810210013235
21	25.01188028024075	27.5266511497936	29.543747040387935	17.917721529577715
22	24.586658277961746	24.397159441727226	29.516179770392668	21.50000250991836
23	21.43169089853404	28.380818199920014	29.488905324206154	20.698585577339788
24	21.263861024147086	27.20730587036439	32.26671563796268	19.262117467525837
25	21.221652563714276	29.34918653545929	29.456234553544085	19.972926347282343
26	21.072228757305954	31.777909037212048	27.270137493327802	19.879724712154196
27	19.864707033962542	30.511446064364346	29.6473230047404	19.97652389693271
28	18.6136382270606	30.01280895003422	31.612630913158497	19.76092190974668
29	19.996645075791168	27.540790356559004	31.29044105958714	21.172123508062697
30	21.283647547224113	27.929785870498254	30.787662579961818	19.998904002315818
31	23.59678830846562	28.093390715644656	27.85444648770391	20.455374488185814
32	23.720736443512614	28.444695622200395	28.174712070532053	19.659855863754938
33	23.351025468978246	28.316773449748922	28.02683604711284	20.30536503415999
34	20.856877761955996	28.349067732656884	29.664599609456705	21.12945489593042
35	20.438348875305582	28.150700518214478	30.241169688887254	21.169780917592686
36	22.73274891278703	28.490919952010362	28.501461609125396	20.274869526077214
37	21.029309153337607	29.545211159431688	29.183950243378415	20.24152944385229
38	20.750791879242808	30.253049969128003	28.084606001382127	20.911552150247058
39	20.863989197311376	29.492502873856523	28.085986456480526	21.557521472351578
40	22.79629167928598	27.838550338086005	28.111294799951143	21.253863182676877
41	19.502693142401053	27.791824024603894	29.188384432482362	23.517098400512694
42	21.355849532067552	27.81290733883396	27.931333653487368	22.899909475611125
43	20.927197308028894	27.80127805042928	28.79746464779989	22.47405999374194
44	20.82709339740876	28.970314358908283	28.94981669229572	21.25277555138723
45	20.615590943545243	29.695806261075013	27.511131487929802	22.177471307449938
46	21.140624032635632	29.603315769482403	28.00140220772419	21.254657990157774
47	20.60927431567076	28.45264369700935	29.20164516782151	21.736436819498383
48	21.096365805541563	27.732422623400137	29.886978376216682	21.284233194841615
49	21.49640496026799	26.817599212889604	30.05229833224291	21.633697494599495
50	20.159873433183463	29.42101203254862	28.652893350222296	21.76622118404562
51	19.818231712316503	29.01072404451591	28.297739902180112	22.87330434098747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	830.0
1	1329.0
2	1828.0
3	7059.5
4	12291.0
5	9622.5
6	6954.0
7	6669.5
8	6385.0
9	6753.0
10	7121.0
11	7343.5
12	7566.0
13	7631.0
14	7696.0
15	7939.0
16	8182.0
17	8459.0
18	8736.0
19	9157.0
20	9578.0
21	10494.0
22	11410.0
23	14100.0
24	16790.0
25	20336.5
26	29199.5
27	34516.0
28	40983.0
29	47450.0
30	56193.5
31	64937.0
32	71542.0
33	78147.0
34	88643.5
35	99140.0
36	105413.0
37	111686.0
38	119210.5
39	126735.0
40	140343.0
41	153951.0
42	162679.5
43	171408.0
44	177935.0
45	184462.0
46	182695.5
47	180929.0
48	181033.0
49	181137.0
50	175226.0
51	169315.0
52	155661.5
53	142008.0
54	130171.5
55	118335.0
56	109693.5
57	101052.0
58	92075.5
59	83099.0
60	77424.0
61	71749.0
62	62003.0
63	52257.0
64	42538.0
65	32819.0
66	27172.0
67	21525.0
68	17142.5
69	12760.0
70	10543.0
71	8326.0
72	6926.5
73	5527.0
74	4740.5
75	3036.5
76	2119.0
77	1541.5
78	964.0
79	680.0
80	396.0
81	322.0
82	248.0
83	222.5
84	197.0
85	130.5
86	64.0
87	46.0
88	28.0
89	22.0
90	16.0
91	11.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2390516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.754608016714794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.93114045175587	15.966756643550239
2	7.06065730804693	2.930823495377334
3	2.394015438912982	1.490605560618071
4	1.28890356040301	1.070027526700502
5	0.9052375735267595	0.9393925500274964
6	0.6591840384742043	0.8208663797644291
7	0.5338301594835442	0.7755604995306911
8	0.4481674112418902	0.7441231156953
9	0.38216819454516315	0.7138575966816415
>10	6.894958525824895	37.05159233825033
>50	2.3510389720540292	32.942679747600465
>100	0.14945805670593737	4.079801976563433
>500	8.268726831727311E-4	0.12446318708364691
>1k	2.067181707931828E-4	0.07705476870121677
>5k	2.067181707931828E-4	0.27239461385524794
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6349	0.265591194537079	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.183197267870201E-5	0.0	0.0	0.023467736672751826	0.0
2	4.183197267870201E-5	0.0	0.0	0.09290881131939716	0.0
3	4.183197267870201E-5	0.0	0.0	0.14712304791099495	0.0
4	4.183197267870201E-5	0.0	0.0	0.25827059931830615	0.0
5	4.183197267870201E-5	0.0	0.0	0.4756713613295205	0.0
6	4.183197267870201E-5	0.0	0.0	0.782048729228334	0.0
7	4.183197267870201E-5	0.0	0.0	0.9367851961668526	0.0
8	4.183197267870201E-5	0.0	0.0	1.2975441285479787	0.0
9	4.183197267870201E-5	0.0	0.0	1.4324940724094715	0.0
10	4.183197267870201E-5	0.0	0.0	1.6555839827049892	0.0
11	4.183197267870201E-5	0.0	0.0	1.9489516071007265	0.0
12	4.183197267870201E-5	0.0	0.0	2.1777306656805475	0.0
13	4.183197267870201E-5	0.0	0.0	2.292182942929476	0.0
14	4.183197267870201E-5	0.0	0.0	2.354177926439313	0.0
15	4.183197267870201E-5	0.0	0.0	2.4267982310095393	0.0
16	4.183197267870201E-5	0.0	0.0	2.5651783966306856	0.0
17	4.183197267870201E-5	0.0	0.0	2.746352670302144	0.0
18	4.183197267870201E-5	0.0	0.0	2.9460585078702675	0.0
19	4.183197267870201E-5	0.0	0.0	3.0872832476335654	0.0
20	4.183197267870201E-5	0.0	0.0	3.2276713479432892	0.0
21	4.183197267870201E-5	0.0	0.0	3.397676484909534	0.0
22	4.183197267870201E-5	0.0	0.0	3.595750875543188	0.0
23	4.183197267870201E-5	0.0	0.0	3.8143647647620846	0.0
24	4.183197267870201E-5	0.0	0.0	3.976589154810091	0.0
25	4.183197267870201E-5	0.0	0.0	4.1153876401580245	0.0
26	4.183197267870201E-5	0.0	0.0	4.261548552697409	0.0
27	4.183197267870201E-5	0.0	0.0	4.403484435996245	0.0
28	4.183197267870201E-5	0.0	0.0	4.56349173149228	0.0
29	4.183197267870201E-5	0.0	0.0	4.73914418477015	0.0
30	1.25495918036106E-4	0.0	0.0	4.9502283189068805	0.0
31	1.25495918036106E-4	0.0	0.0	5.137300900726036	0.0
32	1.25495918036106E-4	0.0	0.0	5.327929200222881	0.0
33	1.25495918036106E-4	0.0	0.0	5.51357949497096	0.0
34	1.25495918036106E-4	0.0	0.0	5.6967617033309965	0.0
35	1.25495918036106E-4	0.0	0.0	5.903411648363784	0.0
36	1.25495918036106E-4	0.0	0.0	6.095545898877062	0.0
37	1.25495918036106E-4	0.0	0.0	6.298179974532695	0.0
38	1.25495918036106E-4	0.0	0.0	6.5103935719317505	0.0
39	1.25495918036106E-4	0.0	0.0	6.769417146758273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	420	0.0	41.25	2
TTTAGCG	170	0.0	41.02941	1
TACGGGA	1120	0.0	40.580357	4
CGACGGT	100	0.0	40.5	28
CGTTTTT	4395	0.0	39.67577	1
TAAGGGA	3840	0.0	39.31641	4
ACGGGAT	1455	0.0	39.27835	5
GCTACGA	150	0.0	39.0	10
GACCGAT	1360	0.0	38.547794	9
ACGTCGG	65	9.094947E-12	38.076927	2
TTAGGGA	4115	0.0	38.055893	4
AGGGCGA	1945	0.0	37.712082	6
AAGGGAT	5045	0.0	37.68583	5
GGGACCG	2010	0.0	37.61194	7
AGGGATG	5125	0.0	37.58049	6
GCGTTAG	295	0.0	37.372883	1
ATAGGGA	4005	0.0	37.359547	4
TAGGGAC	3365	0.0	37.31055	5
GGACCGA	1535	0.0	37.23127	8
TAGGGAT	4655	0.0	37.218044	5
>>END_MODULE