FastQCFastQC Report
Thu 26 May 2016
SRR1547971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547971_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2738194
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202300.7388081341205188No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG253.8922233E-545.01
CGTTTTT115700.042.4330181
CGTAAGG4850.039.8969082
TATCGCG800.039.3751
TACGTCG403.4605546E-739.3751
GCGAACG1150.039.1304361
CGGGATC9650.038.7046626
CGACGGT1050.038.5714328
AGGGCGA20750.038.4939776
GGGCGAT40650.038.4686367
GACCGAT13600.038.382359
GTTAGCG1350.038.3333361
CGTTAGG4300.038.1976742
GGACCGA15600.038.0769278
TTAGGGA45300.037.7483444
ATAGGGA49500.037.681824
AGGGATT66250.037.6641546
TAGGGAC35450.037.5740475
TAAGGGA47350.037.5395974
AAGGGAT60100.037.4376035
Produced by FastQC (version 0.11.5)