##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547971_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2738194
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.820745717797937	31.0	31.0	31.0	30.0	33.0
2	31.144229006418097	31.0	31.0	33.0	30.0	34.0
3	31.217282267070924	31.0	31.0	34.0	28.0	34.0
4	35.15137422695397	35.0	35.0	37.0	32.0	37.0
5	35.01363416909101	35.0	35.0	37.0	32.0	37.0
6	35.063366949164305	37.0	35.0	37.0	32.0	37.0
7	35.513309137336506	35.0	35.0	37.0	35.0	37.0
8	35.615440688278476	37.0	35.0	37.0	35.0	37.0
9	37.42085476777759	39.0	37.0	39.0	35.0	39.0
10	36.53874816758783	38.0	35.0	39.0	32.0	39.0
11	36.074546215498245	38.0	35.0	39.0	31.0	39.0
12	35.82642537380478	37.0	35.0	39.0	30.0	39.0
13	35.72380810125214	37.0	35.0	39.0	30.0	39.0
14	36.75383701812216	39.0	35.0	40.0	31.0	41.0
15	36.89886910861685	39.0	35.0	40.0	31.0	41.0
16	36.970624068272734	39.0	35.0	41.0	31.0	41.0
17	36.899732086185274	38.0	35.0	40.0	31.0	41.0
18	36.777158229110135	38.0	35.0	40.0	31.0	41.0
19	36.71597008831368	38.0	35.0	40.0	31.0	41.0
20	36.56343341633208	38.0	35.0	40.0	30.0	41.0
21	36.463910884327404	38.0	35.0	40.0	30.0	41.0
22	36.341167207290646	38.0	35.0	40.0	30.0	41.0
23	36.25028175505461	38.0	34.0	40.0	30.0	41.0
24	36.148784929044474	38.0	34.0	40.0	30.0	41.0
25	35.99122049058613	38.0	34.0	40.0	29.0	41.0
26	35.76284331935575	38.0	34.0	40.0	29.0	41.0
27	35.826826002832526	38.0	34.0	40.0	29.0	41.0
28	35.82903512315051	38.0	34.0	40.0	29.0	41.0
29	35.80872830778243	38.0	34.0	40.0	29.0	41.0
30	35.72646130989988	38.0	34.0	40.0	29.0	41.0
31	35.63572011333017	38.0	34.0	40.0	28.0	41.0
32	35.490143503345635	38.0	34.0	40.0	27.0	41.0
33	35.304464548530895	38.0	34.0	40.0	27.0	41.0
34	35.10081535493833	38.0	34.0	40.0	25.0	41.0
35	34.929181423960465	38.0	34.0	40.0	25.0	41.0
36	34.84589331508286	38.0	33.0	40.0	24.0	41.0
37	34.79573032444012	38.0	33.0	40.0	24.0	41.0
38	34.722415942771036	38.0	33.0	40.0	24.0	41.0
39	34.672549132749545	38.0	33.0	40.0	24.0	41.0
40	34.52751740745908	38.0	33.0	40.0	23.0	41.0
41	34.441271144411246	38.0	33.0	40.0	23.0	41.0
42	34.41865623838194	37.0	33.0	40.0	23.0	41.0
43	34.38154929855226	37.0	33.0	40.0	23.0	41.0
44	34.24291741198761	37.0	33.0	40.0	23.0	41.0
45	34.1230555614394	37.0	33.0	40.0	23.0	41.0
46	34.05851448071247	37.0	33.0	40.0	23.0	41.0
47	34.01995366288875	37.0	33.0	40.0	23.0	41.0
48	33.92594425376726	37.0	33.0	40.0	23.0	41.0
49	33.78287513594727	37.0	32.0	40.0	22.0	41.0
50	33.67523338375586	36.0	32.0	40.0	22.0	41.0
51	33.353193382207394	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	7.0
14	35.0
15	107.0
16	315.0
17	744.0
18	1571.0
19	2968.0
20	5233.0
21	8201.0
22	12512.0
23	18296.0
24	27400.0
25	39100.0
26	51569.0
27	58813.0
28	65668.0
29	74327.0
30	87059.0
31	104027.0
32	125778.0
33	154262.0
34	211315.0
35	245434.0
36	241490.0
37	295172.0
38	405087.0
39	501651.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.41263913367716	24.899148855048253	28.89696639463822	14.791245616636367
2	31.918702619317695	25.946444992575397	28.818337926385055	13.316514461721852
3	28.226232326854856	25.793716588379056	32.573002497266444	13.407048587499643
4	24.493991294992245	28.960329326556117	31.786279569672566	14.759399808779072
5	21.99727265489589	32.531807461414346	31.12160789191708	14.349311991772678
6	21.16022458598624	40.78918440402689	27.882173432561753	10.16841757742512
7	89.55121514399637	2.423129990059141	6.854992743392177	1.1706621225523102
8	90.01498798112917	1.9751704955894285	6.518603137688564	1.4912383855928397
9	85.10700848807645	5.059721845858986	7.875263768746846	1.958005897317721
10	38.208578354930296	40.78407154496723	12.384257653037002	8.623092447065476
11	24.871941140766506	26.709174003010745	31.24778594942506	17.171098906797692
12	26.416280219736077	24.350502557525143	30.796904821206972	18.43631240153181
13	23.00852313605245	26.956125095592203	31.78916468299909	18.24618708535626
14	18.07443154137362	30.832147028296752	31.756661507548404	19.33675992278122
15	17.742533947558133	29.180949194980343	35.668765617045395	17.40775124041613
16	21.805211756362038	27.999038782496783	34.071289324277245	16.124460136863934
17	21.615634246514308	27.10669879489912	30.196216922540913	21.081450036045656
18	21.468822150658426	27.29463288576339	32.336131041116886	18.9004139224613
19	23.43727288862659	28.373336586085575	29.54863680221343	18.640753723074404
20	24.36602373681339	29.223532006862918	29.33082900627202	17.079615250051678
21	23.93815047436376	27.8277214835764	30.96771083422139	17.26641720783845
22	22.81701004384642	25.214210534388727	31.395401494561742	20.57337792720311
23	19.82397887074473	28.712501743850144	31.198081655280816	20.265437730124308
24	19.67895627555973	28.16359980337405	33.58326692703293	18.57417699403329
25	20.56768074139378	29.621056798751294	30.789162491773777	19.02209996808115
26	19.904652482621756	31.768603685494888	28.843463976621088	19.483279855262264
27	18.940330743548483	30.77900981449817	31.03837785051023	19.24228159144312
28	18.1679603417435	30.071974447391238	32.776019522356705	18.98404568850856
29	19.08352731764075	27.718123697590457	32.63570806159096	20.56264092317783
30	19.892892906784546	28.382758854924084	31.774227830460518	19.950120407830855
31	21.598469648242602	28.574162385864554	29.752019031522238	20.07534893437061
32	21.91389653180162	28.78338788267011	29.92322676917706	19.379488816351216
33	21.192252995952806	29.021391471897168	29.499151630600316	20.28720390154971
34	19.691300178146616	29.178794490090915	30.738837350458002	20.391067981304463
35	19.327264613099	28.98118248743515	30.981369471995045	20.710183427470806
36	21.637071734143014	29.769914038231036	29.38856779322429	19.204446434401653
37	20.05800173398963	30.5245720354365	29.87640758835933	19.54101864221454
38	20.208283269921708	30.954344359822567	28.775901196189896	20.061471174065826
39	19.92495783717297	30.44346017849721	29.095747050793335	20.535834933536485
40	21.85535429556854	28.98439628455836	29.170285231798772	19.98996418807433
41	18.988245537021847	29.503424519957317	29.774625172650293	21.733704770370544
42	20.628815927578543	29.440974598585782	28.601589222677433	21.328620251158245
43	20.437704560012914	29.187413309648626	29.309866284127423	21.065015846211043
44	20.557455023274464	29.88999318528928	28.83849719924885	20.714054592187406
45	19.755539600189028	30.681463767724278	28.11842404153979	21.44457259054691
46	20.52546313372975	30.373669652332886	28.606154275409267	20.494712938528096
47	19.77770749625483	29.3217354212302	30.015842559000568	20.884714523514404
48	20.41915218571073	28.75859051623077	30.145928301646997	20.676328996411502
49	20.425689341222718	28.31041189922993	29.708815372468127	21.555083387079222
50	19.3470952021661	30.07668558181049	30.016499926593955	20.559719289429456
51	18.959321362912927	29.719552376493414	29.379182044807635	21.941944215786023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1254.0
1	1762.5
2	2271.0
3	18082.0
4	33893.0
5	25651.5
6	17410.0
7	16267.5
8	15125.0
9	16356.5
10	17588.0
11	18223.0
12	18858.0
13	19374.5
14	19891.0
15	19326.0
16	18761.0
17	17937.0
18	17113.0
19	17189.0
20	17265.0
21	18182.0
22	19099.0
23	21086.0
24	23073.0
25	25989.0
26	34878.5
27	40852.0
28	48061.0
29	55270.0
30	62930.5
31	70591.0
32	79265.5
33	87940.0
34	98446.0
35	108952.0
36	117179.5
37	125407.0
38	134848.5
39	144290.0
40	156351.0
41	168412.0
42	181147.0
43	193882.0
44	201383.0
45	208884.0
46	215033.0
47	221182.0
48	216142.5
49	211103.0
50	199269.5
51	187436.0
52	173817.5
53	160199.0
54	146131.5
55	132064.0
56	117107.0
57	102150.0
58	93005.0
59	83860.0
60	74821.0
61	65782.0
62	55412.5
63	45043.0
64	37354.5
65	29666.0
66	23848.0
67	18030.0
68	14366.5
69	10703.0
70	8439.0
71	6175.0
72	4914.5
73	3654.0
74	3325.0
75	2264.5
76	1533.0
77	1075.5
78	618.0
79	531.0
80	444.0
81	356.5
82	269.0
83	229.5
84	190.0
85	125.0
86	60.0
87	42.0
88	24.0
89	17.0
90	10.0
91	9.0
92	8.0
93	7.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2738194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.294787036207772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62198526473163	17.61598041997166
2	7.204929478730768	3.3567459563585746
3	2.866183018726174	2.0030136908406404
4	1.6799598182091509	1.5653722477827396
5	1.1525949765790042	1.3424727259205396
6	0.855918739394102	1.1963066852691016
7	0.6687704069015656	1.0905204943422995
8	0.5466070626264429	1.0186476093096057
9	0.4786674032064467	1.0035409696981896
>10	7.268771418429451	42.32504609360339
>50	1.5845154801283623	23.966665026476385
>100	0.06883522725433752	2.2929227195556128
>500	0.0014539532673812489	0.24901632379925204
>1k	6.462014521694439E-4	0.2124372295370678
>5k	0.0	0.0
>10k+	1.6155036304236098E-4	0.7613118075349659
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20230	0.7388081341205188	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037469952822919046	0.0
2	0.0	0.0	0.0	0.12343902586887562	0.0
3	0.0	0.0	0.0	0.20115448357567067	0.0
4	0.0	0.0	0.0	0.32148927358689705	0.0
5	0.0	0.0	0.0	0.6189115891715489	0.0
6	0.0	0.0	0.0	1.1204830629239564	0.0
7	0.0	0.0	0.0	1.4126099173396771	0.0
8	0.0	0.0	0.0	1.9537330079607216	0.0
9	0.0	0.0	0.0	2.1971416196222764	0.0
10	0.0	0.0	0.0	2.504497489951406	0.0
11	0.0	0.0	0.0	2.8328891232688407	0.0
12	0.0	0.0	0.0	3.0897007297510695	0.0
13	0.0	0.0	0.0	3.2327147017340625	0.0
14	0.0	0.0	0.0	3.3218245310595234	0.0
15	0.0	0.0	0.0	3.407647522418061	0.0
16	0.0	0.0	0.0	3.537441101689654	0.0
17	0.0	0.0	0.0	3.702111683832482	0.0
18	0.0	0.0	0.0	3.8957064400842305	0.0
19	0.0	0.0	0.0	4.032694542461199	0.0
20	0.0	0.0	0.0	4.172494717320979	0.0
21	0.0	0.0	0.0	4.334134104449867	0.0
22	0.0	0.0	0.0	4.5359459556189226	0.0
23	0.0	0.0	0.0	4.746960953095361	0.0
24	0.0	0.0	0.0	4.922076375888633	0.0
25	0.0	0.0	0.0	5.0706414519935405	0.0
26	0.0	0.0	0.0	5.211938964149363	0.0
27	0.0	0.0	0.0	5.361015326160236	0.0
28	0.0	0.0	0.0	5.5185279056195435	0.0
29	0.0	0.0	0.0	5.696747564270464	0.0
30	0.0	0.0	0.0	5.911560685619792	0.0
31	0.0	0.0	0.0	6.101722522217199	0.0
32	0.0	0.0	0.0	6.290058337721871	0.0
33	0.0	0.0	0.0	6.47090746674633	0.0
34	0.0	0.0	0.0	6.64920016624096	0.0
35	0.0	0.0	0.0	6.851085058253725	0.0
36	0.0	0.0	0.0	7.047564927831995	0.0
37	0.0	0.0	0.0	7.242583980536076	0.0
38	0.0	0.0	0.0	7.450275619623738	0.0
39	0.0	0.0	0.0	7.7145008717424695	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	25	3.8922233E-5	45.0	1
CGTTTTT	11570	0.0	42.433018	1
CGTAAGG	485	0.0	39.896908	2
TATCGCG	80	0.0	39.375	1
TACGTCG	40	3.4605546E-7	39.375	1
GCGAACG	115	0.0	39.130436	1
CGGGATC	965	0.0	38.704662	6
CGACGGT	105	0.0	38.57143	28
AGGGCGA	2075	0.0	38.493977	6
GGGCGAT	4065	0.0	38.468636	7
GACCGAT	1360	0.0	38.38235	9
GTTAGCG	135	0.0	38.333336	1
CGTTAGG	430	0.0	38.197674	2
GGACCGA	1560	0.0	38.076927	8
TTAGGGA	4530	0.0	37.748344	4
ATAGGGA	4950	0.0	37.68182	4
AGGGATT	6625	0.0	37.664154	6
TAGGGAC	3545	0.0	37.574047	5
TAAGGGA	4735	0.0	37.539597	4
AAGGGAT	6010	0.0	37.437603	5
>>END_MODULE