FastQCFastQC Report
Thu 26 May 2016
SRR1547970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547970_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1921930
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74410.38716290395591935No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24860.12934914382937984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA207.034473E-445.022
CGCGTAG207.034473E-445.02
TATCGGC207.034473E-445.043
GTCGATC302.1662672E-644.99999627
TCTCACG2850.042.6315823
CGACGGT2750.041.72727228
CGGTCTA2550.041.4705931
TCGTTAG603.6379788E-1241.2499961
CGTTTTT46650.041.141481
CACGACG2700.040.83333226
CGACAGG1600.040.781252
TAGTACG451.929402E-840.0000041
AGGGCGA13550.039.022146
TCACGAC2900.038.79310225
TAGGGTA14850.038.787885
AAGGGTA12850.038.3463025
GACCGAT9550.038.167549
TACGGGA6200.038.104844
AGGGTAC12100.037.9338876
TTCGTAG1250.037.8000031
Produced by FastQC (version 0.11.5)