FastQCFastQC Report
Thu 26 May 2016
SRR1547969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547969_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2139457
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255771.1954902575747024No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.11301933154066664No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT130650.042.4339831
TATTGCG1350.041.6666641
GTACGTC451.929584E-840.00000427
TACGGGA11600.039.7629284
GGGCGAT39050.039.756727
CGTTAGG2400.039.3749962
GCGCGAC1500.039.09
TAGGGAT42500.038.9117665
AGGGCGA19700.038.7182776
TAATGCG1050.038.571431
TTAGGGA32300.038.173374
TAGGGCG8600.037.9360475
TAGGGAC24800.037.7419365
AAGGGAT44200.037.7205855
ACGGGAT10450.037.6794245
GTAGGGA36750.037.4693874
CACGACC4750.037.4210527
TAAGGGA32900.037.1352584
GGCGATC5600.036.9642838
GGACCGA12250.036.918378
Produced by FastQC (version 0.11.5)