FastQCFastQC Report
Thu 26 May 2016
SRR1547968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547968_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1611164
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60230.37382910740309494No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36450.22623395259576307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTA453.8562575E-1045.00000419
CGACATA207.034131E-445.00000444
GTCGTTG207.034131E-445.0000041
CGGATAC253.8915066E-545.019
TATCCGT253.8915066E-545.014
ATACGCA302.1660471E-644.99999613
CGATATA302.1660471E-644.99999610
AATACGC302.1660471E-644.99999612
CGGTCTA2500.043.19999731
CGACGGT2700.042.50000428
TCGATAG800.042.1875041
TATTGCG1150.041.086961
TCTCACG2800.040.9821423
ACGGTCT2700.040.83333630
CGTTTTT32500.040.7769241
CGTAAGG2500.040.4999962
ACGTTAG451.9292202E-840.0000041
TCACGAC2900.039.56896625
CACGACG2900.039.56896626
TAATGCG800.039.3750041
Produced by FastQC (version 0.11.5)