##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547968_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1611164
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.825663929928922	31.0	31.0	31.0	30.0	33.0
2	31.16170545022108	31.0	31.0	33.0	30.0	34.0
3	31.340177039705456	31.0	31.0	34.0	30.0	34.0
4	35.20982718084565	35.0	35.0	37.0	33.0	37.0
5	35.03096581105338	35.0	35.0	37.0	32.0	37.0
6	35.05451524487886	37.0	35.0	37.0	32.0	37.0
7	35.48444664851002	35.0	35.0	37.0	35.0	37.0
8	35.566937319850744	37.0	35.0	37.0	35.0	37.0
9	37.38227269228955	39.0	37.0	39.0	35.0	39.0
10	36.46750237716334	38.0	35.0	39.0	32.0	39.0
11	36.05976983100417	38.0	35.0	39.0	31.0	39.0
12	35.326225014958126	37.0	35.0	39.0	30.0	39.0
13	34.92208241991504	37.0	34.0	39.0	27.0	39.0
14	35.888610966977915	38.0	34.0	40.0	27.0	41.0
15	36.190560365052846	38.0	34.0	40.0	30.0	41.0
16	36.353884520756424	38.0	35.0	40.0	31.0	41.0
17	36.29426985707228	38.0	34.0	40.0	30.0	41.0
18	36.22417829594008	38.0	35.0	40.0	30.0	41.0
19	36.157287526285344	38.0	34.0	40.0	30.0	41.0
20	36.02830251917248	38.0	34.0	40.0	30.0	41.0
21	35.88782644100787	37.0	34.0	40.0	30.0	41.0
22	35.72929385214665	37.0	34.0	40.0	29.0	41.0
23	35.69404107837563	37.0	34.0	40.0	29.0	41.0
24	35.55994920442612	37.0	34.0	40.0	29.0	41.0
25	35.39088509922019	37.0	34.0	40.0	29.0	41.0
26	35.14570707885727	36.0	34.0	40.0	27.0	41.0
27	35.177881332999	37.0	34.0	40.0	27.0	41.0
28	35.236672368548454	37.0	34.0	40.0	28.0	41.0
29	35.23289683731762	36.0	34.0	40.0	28.0	41.0
30	35.138262771511776	36.0	34.0	40.0	27.0	41.0
31	35.01370686038169	36.0	34.0	40.0	27.0	41.0
32	34.86951731791425	36.0	33.0	40.0	27.0	41.0
33	34.73171384166975	36.0	33.0	40.0	26.0	41.0
34	34.56676477379087	36.0	33.0	40.0	25.0	41.0
35	34.439616327077815	36.0	33.0	40.0	24.0	41.0
36	34.31070331760144	36.0	33.0	40.0	24.0	41.0
37	34.23809990789268	36.0	33.0	40.0	24.0	41.0
38	34.19197983569643	36.0	33.0	40.0	24.0	41.0
39	34.164237160214604	36.0	33.0	40.0	23.0	41.0
40	33.94819335585949	36.0	33.0	40.0	23.0	41.0
41	33.89069703642832	36.0	33.0	40.0	23.0	41.0
42	33.84421201069537	35.0	33.0	40.0	23.0	41.0
43	33.777073593997876	35.0	33.0	40.0	23.0	41.0
44	33.58225605835284	35.0	32.0	40.0	23.0	41.0
45	33.440317062695044	35.0	32.0	40.0	23.0	41.0
46	33.39169941731568	35.0	32.0	39.0	23.0	41.0
47	33.33591366242046	35.0	32.0	39.0	22.0	41.0
48	33.25755913116232	35.0	32.0	39.0	22.0	41.0
49	33.12499162096472	35.0	32.0	39.0	22.0	41.0
50	32.97887490038258	35.0	31.0	39.0	21.0	41.0
51	32.62790628390406	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	1.0
13	7.0
14	39.0
15	97.0
16	283.0
17	612.0
18	1264.0
19	2358.0
20	3968.0
21	6224.0
22	9052.0
23	13015.0
24	18362.0
25	23973.0
26	30389.0
27	35974.0
28	41333.0
29	48858.0
30	57471.0
31	68695.0
32	83141.0
33	104590.0
34	158757.0
35	182700.0
36	134085.0
37	153979.0
38	199964.0
39	231942.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.828479285783445	25.323679029571167	30.472565176481105	12.375276508164283
2	29.50556243808824	27.827024436990893	28.98165549875742	13.685757626163445
3	28.805323356281548	26.52206727558461	30.902192452165018	13.770416915968827
4	25.887495003612294	28.934732901182002	29.74532698099014	15.432445114215563
5	23.58133622647974	33.061376743770346	28.919216169179553	14.43807086057037
6	21.427800025323307	42.420821219937885	25.64183410254946	10.509544652189348
7	90.4004806462905	3.6399770600634076	4.711872906792853	1.2476693868532316
8	91.0716724057886	2.944020596289391	4.331092303452659	1.65321469446934
9	87.10137515485698	5.285060986963462	5.689178755235345	1.924385102944207
10	45.7540635217768	29.90719752923973	12.347780859055938	11.990958089927531
11	39.26993155259179	24.514636622963273	21.147443711502987	15.067988112941947
12	37.375090307380255	23.486994495904824	22.843856987867156	16.294058208847765
13	24.199212494817413	33.90319048836742	24.17395125511742	17.723645761697753
14	18.653284209428712	37.48681077779791	25.88482612570787	17.9750788870655
15	18.091640577867928	27.237202420113654	37.65631555819271	17.014841443825706
16	20.666797420995007	24.70350628489713	37.3203472768756	17.30934901723226
17	20.959380919633258	25.158953402633127	25.476115404763267	28.40555027297035
18	23.406493690276097	27.505579816828078	29.724224225466806	19.363702267429016
19	27.464677711269616	29.015668175306796	23.681698449071604	19.837955664351984
20	29.097782721063776	28.525711845597346	23.766295671948974	18.6102097613899
21	24.747573803784096	29.974416012274357	26.586678947642824	18.691331236298726
22	24.25463826153017	26.836125931314253	25.43800631096524	23.471229496190332
23	22.30561258816607	31.312578980165895	24.705368292737425	21.676440138930612
24	22.340866603275643	27.70363538410739	32.11777323723718	17.837724775379787
25	21.09083867315804	29.153891223984647	28.90332703560904	20.851943067248275
26	19.8684305260048	34.176098770826556	24.58557912167849	21.36989158149015
27	19.643934447393313	33.825855096067194	26.952625555188668	19.577584901350825
28	17.485370825068085	32.08363642683178	31.36937021929487	19.061622528805263
29	19.42322445139042	28.59646814352853	30.858062866350046	21.122244538731007
30	21.084383712645018	31.433981891353085	28.410143225643075	19.07149117035882
31	26.67046930045607	29.696294107862393	24.383675404862572	19.24956118681897
32	27.817466130077385	29.44914360052732	24.661735242346527	18.071655027048767
33	26.177161356634087	29.87616406523482	23.85021015861824	20.09646441951285
34	20.15803481209858	30.229200751754632	27.665526290309366	21.94723814583742
35	21.253205756831704	28.790737628199242	28.571144836900526	21.384911778068528
36	27.50601428532415	29.635840919980833	24.76309053578655	18.095054258908466
37	21.660488938432092	32.72658773408542	26.649801013428803	18.96312231405369
38	21.61095952988026	33.40553785958475	23.589529060977032	21.39397354955796
39	21.329734279067804	31.861374757628646	25.73108634502757	21.07780461827598
40	24.300257453617384	28.36483436819591	25.306672691296477	22.028235486890225
41	19.056160639140398	27.582604874488258	27.1014620485562	26.259772437815144
42	22.587706775970666	28.855038965617403	24.350035129881253	24.207219128530678
43	22.086764600003477	28.752938869041266	25.33857509229352	23.82172143866174
44	21.204048749847935	30.070681817617572	26.44771109582885	22.277558336705635
45	19.98114406727062	34.582761283146844	22.69135854574705	22.74473610383549
46	22.494544317027938	32.64751446780092	24.698230596016295	20.159710619154847
47	21.544237582269712	30.137527899084137	26.25530361899844	22.06293089964771
48	22.713019903622474	27.94488953328153	28.81258518685869	20.529505376237303
49	22.066654915328296	27.219699546414887	28.802778612233144	21.91086692602367
50	20.53037431322944	31.55780541273266	26.89422057593144	21.017599698106462
51	19.43464476614423	32.33420061520739	24.855942660089227	23.375211958559152
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1058.0
1	1503.0
2	1948.0
3	6017.5
4	10087.0
5	7920.5
6	5754.0
7	5907.0
8	6060.0
9	7484.0
10	8908.0
11	9981.5
12	11055.0
13	11409.0
14	11763.0
15	11063.5
16	10364.0
17	10038.0
18	9712.0
19	9551.5
20	9391.0
21	9283.5
22	9176.0
23	10001.5
24	10827.0
25	11214.5
26	12899.5
27	14197.0
28	16881.0
29	19565.0
30	23241.0
31	26917.0
32	30657.5
33	34398.0
34	38525.5
35	42653.0
36	46207.0
37	49761.0
38	56532.5
39	63304.0
40	80708.0
41	98112.0
42	111162.0
43	124212.0
44	126998.0
45	129784.0
46	133445.5
47	137107.0
48	132180.5
49	127254.0
50	121724.0
51	116194.0
52	107061.5
53	97929.0
54	90785.5
55	83642.0
56	78131.0
57	72620.0
58	69911.5
59	67203.0
60	61845.5
61	56488.0
62	51409.0
63	46330.0
64	38538.0
65	30746.0
66	25582.0
67	20418.0
68	16372.5
69	12327.0
70	10856.5
71	9386.0
72	7405.5
73	5425.0
74	4378.0
75	2520.5
76	1710.0
77	1430.0
78	1150.0
79	829.5
80	509.0
81	462.0
82	415.0
83	313.0
84	211.0
85	142.5
86	74.0
87	56.0
88	38.0
89	32.0
90	26.0
91	20.0
92	14.0
93	9.5
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1611164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.99217748559442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91380922357614	17.57478675994819
2	7.388522137383453	3.2497938040316074
3	2.6330861656571116	1.7372189486998342
4	1.2713748136655176	1.1184120221138638
5	0.809009923218749	0.8895944909516921
6	0.5685949855891976	0.7502785104297982
7	0.4084627846139807	0.6288090238843552
8	0.3301037284060516	0.5807759827009875
9	0.26110640119945155	0.5168068486022847
>10	3.729484984437934	21.00952991274357
>50	2.0041053066828027	31.84553122745993
>100	0.6794592604470034	19.143302188453106
>500	0.0023042280981097995	0.34275298593912734
>1k	2.8802851226372494E-4	0.23088793822733678
>5k	2.8802851226372494E-4	0.3815193558143345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6023	0.37382910740309494	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3645	0.22623395259576307	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.206692800981155E-5	0.0	0.0	0.025943975908101224	0.0
2	6.206692800981155E-5	0.0	0.0	0.11103773420955285	0.0
3	6.206692800981155E-5	0.0	0.0	0.1726701937232957	0.0
4	6.206692800981155E-5	0.0	0.0	0.32833404917190306	0.0
5	6.206692800981155E-5	0.0	0.0	0.6126626463848497	0.0
6	6.206692800981155E-5	0.0	0.0	0.9811539979791009	0.0
7	6.206692800981155E-5	0.0	0.0	1.159968817575368	0.0
8	6.206692800981155E-5	0.0	0.0	1.5495008577649452	0.0
9	6.206692800981155E-5	0.0	0.0	1.6926271937555706	0.0
10	6.206692800981155E-5	0.0	0.0	1.9468533308837586	0.0
11	6.206692800981155E-5	0.0	0.0	2.3153446824780097	0.0
12	6.206692800981155E-5	0.0	0.0	2.578446390311601	0.0
13	6.206692800981155E-5	0.0	0.0	2.695939085034174	0.0
14	6.206692800981155E-5	0.0	0.0	2.749564910834651	0.0
15	6.206692800981155E-5	0.0	0.0	2.8258451653587096	0.0
16	6.206692800981155E-5	0.0	0.0	2.994356874905348	0.0
17	6.206692800981155E-5	0.0	0.0	3.1798749227266745	0.0
18	6.206692800981155E-5	0.0	0.0	3.3971712376890246	0.0
19	6.206692800981155E-5	0.0	0.0	3.5217395622047167	0.0
20	6.206692800981155E-5	0.0	0.0	3.6468664890724964	0.0
21	6.206692800981155E-5	0.0	0.0	3.8185436119476353	0.0
22	6.206692800981155E-5	0.0	0.0	3.997544632327932	0.0
23	6.206692800981155E-5	0.0	0.0	4.188648703670141	0.0
24	6.206692800981155E-5	0.0	0.0	4.336740393901552	0.0
25	6.206692800981155E-5	0.0	0.0	4.46248799004943	0.0
26	6.206692800981155E-5	0.0	0.0	4.589538991685514	0.0
27	6.206692800981155E-5	0.0	0.0	4.716776194105628	0.0
28	6.206692800981155E-5	0.0	0.0	4.843640994957683	0.0
29	6.206692800981155E-5	0.0	0.0	4.993656759957397	0.0
30	6.206692800981155E-5	0.0	0.0	5.168933764657105	0.0
31	6.206692800981155E-5	0.0	0.0	5.332976655387037	0.0
32	6.206692800981155E-5	0.0	0.0	5.48007527477029	0.0
33	6.206692800981155E-5	0.0	0.0	5.638966610475408	0.0
34	6.206692800981155E-5	0.0	0.0	5.804809442117624	0.0
35	6.206692800981155E-5	0.0	0.0	6.001871938548776	0.0
36	6.206692800981155E-5	0.0	0.0	6.175224868480179	0.0
37	6.206692800981155E-5	0.0	0.0	6.357205101404947	0.0
38	6.206692800981155E-5	0.0	0.0	6.55054358215551	0.0
39	6.206692800981155E-5	0.0	0.0	6.79068052662547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGCTA	45	3.8562575E-10	45.000004	19
CGACATA	20	7.034131E-4	45.000004	44
GTCGTTG	20	7.034131E-4	45.000004	1
CGGATAC	25	3.8915066E-5	45.0	19
TATCCGT	25	3.8915066E-5	45.0	14
ATACGCA	30	2.1660471E-6	44.999996	13
CGATATA	30	2.1660471E-6	44.999996	10
AATACGC	30	2.1660471E-6	44.999996	12
CGGTCTA	250	0.0	43.199997	31
CGACGGT	270	0.0	42.500004	28
TCGATAG	80	0.0	42.187504	1
TATTGCG	115	0.0	41.08696	1
TCTCACG	280	0.0	40.98214	23
ACGGTCT	270	0.0	40.833336	30
CGTTTTT	3250	0.0	40.776924	1
CGTAAGG	250	0.0	40.499996	2
ACGTTAG	45	1.9292202E-8	40.000004	1
TCACGAC	290	0.0	39.568966	25
CACGACG	290	0.0	39.568966	26
TAATGCG	80	0.0	39.375004	1
>>END_MODULE