FastQCFastQC Report
Thu 26 May 2016
SRR1547967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547967_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2066142
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259541.2561576116259192No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.11446454309529548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGC207.034596E-445.029
CGTTTTT146150.042.613751
CGACGGT750.039.00000428
GGCGATA6700.038.9552238
TAGGGAT38250.038.7058835
TACGGGA9050.038.535914
GCGATCG2050.038.4146359
CACGACC3350.038.2835827
TAAGGGA34500.038.0869564
TAGGGAC24100.037.9045645
TCGCGTA301.14023715E-437.49999630
TTAGGGA33150.037.3981934
AGGGCGA17550.037.307696
GGGCGAT34600.037.1315047
AAGGGAT42000.037.1255
CACGGGA5350.037.0093464
TAGGGCG6100.036.8852425
ACGGGAT11550.036.8181845
AGGGATT45200.036.6869476
ACGGGAC6950.036.582735
Produced by FastQC (version 0.11.5)