##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547967_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2066142
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.865457456457495	31.0	31.0	31.0	30.0	33.0
2	31.151814831700822	31.0	31.0	33.0	30.0	34.0
3	31.097837418725334	31.0	31.0	34.0	28.0	34.0
4	35.11443405148339	35.0	35.0	37.0	32.0	37.0
5	35.0503493951529	35.0	35.0	37.0	32.0	37.0
6	35.12510369568016	37.0	35.0	37.0	32.0	37.0
7	35.56371536903078	36.0	35.0	37.0	35.0	37.0
8	35.664778606697894	37.0	35.0	37.0	35.0	37.0
9	37.48232744893623	39.0	37.0	39.0	35.0	39.0
10	36.64834314388847	39.0	37.0	39.0	32.0	39.0
11	36.21680746047464	38.0	35.0	39.0	31.0	39.0
12	35.948192815401846	38.0	35.0	39.0	31.0	39.0
13	35.85307011812354	38.0	35.0	39.0	30.0	39.0
14	36.90263253929304	39.0	35.0	41.0	31.0	41.0
15	37.01558121368231	39.0	35.0	41.0	31.0	41.0
16	37.07370112993202	39.0	35.0	41.0	31.0	41.0
17	36.98773317613213	39.0	35.0	41.0	31.0	41.0
18	36.815800656489245	39.0	35.0	40.0	31.0	41.0
19	36.707237934275575	38.0	35.0	40.0	31.0	41.0
20	36.5083479257476	38.0	35.0	40.0	30.0	41.0
21	36.40317025644897	38.0	35.0	40.0	30.0	41.0
22	36.30139215988059	38.0	35.0	40.0	30.0	41.0
23	36.21587480434549	38.0	34.0	40.0	30.0	41.0
24	36.100490188960876	38.0	34.0	40.0	30.0	41.0
25	35.93930959246751	38.0	34.0	40.0	30.0	41.0
26	35.71856387411901	38.0	34.0	40.0	29.0	41.0
27	35.77589633239148	38.0	34.0	40.0	29.0	41.0
28	35.76884889809123	38.0	34.0	40.0	29.0	41.0
29	35.715808013195605	38.0	34.0	40.0	29.0	41.0
30	35.600473733170325	38.0	34.0	40.0	29.0	41.0
31	35.46311628145597	38.0	34.0	40.0	28.0	41.0
32	35.250075745035915	38.0	34.0	40.0	27.0	41.0
33	34.971086208014746	38.0	33.0	40.0	25.0	41.0
34	34.625502022610256	38.0	33.0	40.0	24.0	41.0
35	34.3355916485895	37.0	33.0	40.0	23.0	41.0
36	34.24229360808695	37.0	33.0	40.0	22.0	41.0
37	34.180926577166524	37.0	33.0	40.0	22.0	41.0
38	34.075053408720215	37.0	33.0	40.0	21.0	41.0
39	34.009927197646626	37.0	33.0	40.0	21.0	41.0
40	33.861084572115566	37.0	33.0	40.0	20.0	41.0
41	33.77119530022622	37.0	33.0	40.0	20.0	41.0
42	33.726140797679925	37.0	33.0	40.0	19.0	41.0
43	33.67950315128389	37.0	33.0	40.0	19.0	41.0
44	33.4938450503402	37.0	32.0	40.0	18.0	41.0
45	33.36171328011337	36.0	32.0	40.0	18.0	41.0
46	33.32143821673438	36.0	32.0	40.0	18.0	41.0
47	33.2729275141786	36.0	32.0	40.0	18.0	41.0
48	33.196014117132314	36.0	32.0	40.0	18.0	41.0
49	33.06062264839493	36.0	31.0	40.0	18.0	41.0
50	32.98393721244716	36.0	31.0	40.0	18.0	41.0
51	32.696775439442206	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	4.0
13	10.0
14	28.0
15	98.0
16	249.0
17	667.0
18	1407.0
19	2719.0
20	4537.0
21	7493.0
22	11323.0
23	16256.0
24	24361.0
25	36355.0
26	47508.0
27	52481.0
28	55103.0
29	58641.0
30	66520.0
31	77450.0
32	92858.0
33	114197.0
34	157834.0
35	181384.0
36	182499.0
37	218973.0
38	296306.0
39	358837.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.09123187080075	22.978817525610534	27.766532987568134	18.16341761602058
2	34.409106440893225	25.071461690435605	27.72674869394262	12.792683174728554
3	27.91274752654948	25.26452683310247	34.087250537475164	12.73547510287289
4	24.160004491462832	27.596070357216497	34.17417583109002	14.06974932023065
5	22.09354439336696	31.828112491784204	32.736956124022456	13.341386990826381
6	20.90809828172507	40.34098333996405	29.233276318859012	9.517642059451866
7	86.79635765595975	2.512412022019784	9.661533428002528	1.029696894017933
8	87.3616624607602	2.0775435570256064	9.404726296643696	1.1560676855704979
9	82.58033571748699	5.056525640541647	10.562826756341046	1.8003118856303197
10	37.1629829895525	38.265859752137075	15.542978168973864	9.02817908933655
11	25.751424635867238	25.725676163593793	31.680252373747784	16.84264682679119
12	26.20802442426513	23.576646716440592	32.45517491053374	17.76015394876054
13	23.57040319590812	25.453816823819466	32.880605495653256	18.095174484619157
14	18.842799768844543	28.577464666029726	33.32050749658058	19.25922806854514
15	18.52699378842306	27.712519275054664	36.84678981405924	16.913697122463027
16	23.46629612098297	26.636358972422997	34.47580079200752	15.42154411458651
17	22.30848605758946	26.378196658312937	32.40600113641754	18.907316147680074
18	22.419272247502832	26.81708227217684	33.15677238060114	17.606873099719188
19	24.022066247140806	28.19370595051066	30.371436232359635	17.412791569988897
20	25.18423225509186	28.417214305696316	30.71647544070059	15.682077998511234
21	24.59947089793441	26.784073892307497	32.22842379662192	16.388031413136172
22	23.47321723289106	24.780678191527976	32.880266699965446	18.86583787561552
23	20.642821258171026	27.72248954815303	32.975565087007574	18.659124106668372
24	20.52008042041641	27.339505222777525	34.377017649319356	17.763396707486706
25	21.58055932264094	28.222842379662193	31.720811057516862	18.475787240180008
26	20.393951625783707	30.142652344320965	30.489627528020826	18.973768501874506
27	19.24979018867048	29.462011807513715	32.725775866324774	18.562422137491033
28	18.517362311012505	29.430600607315473	33.58781729426148	18.464219787410546
29	19.8883716608055	28.456030611642376	33.16848503152252	18.487112696029605
30	21.238278879186424	27.493318465042577	33.00358833032773	18.264814325443265
31	22.34681836969579	28.382995941227662	31.103960908785556	18.166224780290996
32	23.131420783276273	28.386964690713416	30.826729237390264	17.654885288620044
33	22.301806942601235	28.99350577065855	30.35667442024798	18.348012866492237
34	20.619541154480185	29.725740050780637	31.442514599674176	18.212204195065006
35	20.379770606279724	30.247533809389676	30.706021173762498	18.666674410568103
36	21.5696694612471	31.6773968100934	28.37984998126944	18.373083747390062
37	21.306086416132093	31.853812564673678	28.607617482244684	18.23248353694954
38	21.342095557807742	31.434722298854577	28.196900309852857	19.026281833484823
39	20.849777024038037	30.748661031042396	28.31078406034048	20.090777884579087
40	22.168757036060445	29.409401677135456	29.034451649499402	19.387389637304697
41	19.31798492068793	30.15746255581659	29.365793832176102	21.158758691319377
42	20.47957013603131	30.53889810090497	28.534437613678055	20.447094149385666
43	20.973679447008	29.740840658580098	28.71322493807299	20.572254956338913
44	20.395016412231104	30.597074160440087	28.673343845679533	20.33456558164928
45	20.352086158647374	30.80795027640888	28.231312271857405	20.608651293086343
46	20.9207789203259	30.796382823639423	28.14937211479172	20.133466141242952
47	19.99243033634668	30.602543290828994	29.542499983060218	19.862526389764113
48	20.725923000452052	29.502570491282786	29.86348469756677	19.908021810698393
49	20.79406933308553	29.3336082418343	29.460656624762482	20.411665800317692
50	19.70469599862933	30.227399665657057	29.87379376635294	20.194110569360674
51	19.10265606139365	30.507293303170833	29.4956493793747	20.894401256060814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	973.0
1	1595.5
2	2218.0
3	22978.5
4	43739.0
5	32966.0
6	22193.0
7	20484.0
8	18775.0
9	19572.0
10	20369.0
11	21425.5
12	22482.0
13	22747.0
14	23012.0
15	22093.0
16	21174.0
17	19714.0
18	18254.0
19	17327.0
20	16400.0
21	16707.5
22	17015.0
23	17670.0
24	18325.0
25	20198.0
26	25572.0
27	29073.0
28	33612.0
29	38151.0
30	43363.0
31	48575.0
32	54998.0
33	61421.0
34	67480.0
35	73539.0
36	79545.0
37	85551.0
38	91896.5
39	98242.0
40	105778.0
41	113314.0
42	120555.0
43	127796.0
44	131009.0
45	134222.0
46	137150.0
47	140078.0
48	137379.5
49	134681.0
50	133426.5
51	132172.0
52	124772.0
53	117372.0
54	109589.5
55	101807.0
56	95223.5
57	88640.0
58	83246.5
59	77853.0
60	71512.0
61	65171.0
62	56502.5
63	47834.0
64	39512.5
65	31191.0
66	25619.0
67	20047.0
68	16360.5
69	12674.0
70	10138.5
71	7603.0
72	6355.0
73	5107.0
74	4216.0
75	2646.0
76	1967.0
77	1427.5
78	888.0
79	657.5
80	427.0
81	314.5
82	202.0
83	160.0
84	118.0
85	80.5
86	43.0
87	36.0
88	29.0
89	22.0
90	15.0
91	10.5
92	6.0
93	3.0
94	0.0
95	3.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2066142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.03932004534867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.56806737693987	19.08112558786806
2	7.632962051610841	4.127802076150135
3	3.542360427523568	2.873490519473637
4	2.2445237634071127	2.4276158555262155
5	1.603503639014649	2.1678824044599168
6	1.279776854726371	2.0762577576945622
7	1.0426172327726502	1.9734162729214837
8	0.9687383817644523	2.095522171579375
9	0.8387808224117953	2.0412056794583973
>10	10.100587588848727	55.86614944546543
>50	0.15576959082178637	2.588861317413997
>100	0.02030060403658951	0.9678506532609117
>500	0.0014630299066325278	0.25945978109432727
>1k	3.6575747665813194E-4	0.169956788187765
>5k	0.0	0.0
>10k+	1.8287873832906597E-4	1.2834036894458107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25954	1.2561576116259192	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2365	0.11446454309529548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8399383972640794E-5	0.0	0.0	0.014326217655901675	0.0
2	4.8399383972640794E-5	0.0	0.0	0.037945117034550384	0.0
3	4.8399383972640794E-5	0.0	0.0	0.07603543222101869	0.0
4	4.8399383972640794E-5	0.0	0.0	0.11504533570296717	0.0
5	4.8399383972640794E-5	0.0	0.0	0.2385605636011465	0.0
6	4.8399383972640794E-5	0.0	0.0	0.4993364444457351	0.0
7	4.8399383972640794E-5	0.0	0.0	0.6716382513883363	0.0
8	4.8399383972640794E-5	0.0	0.0	0.9862826465944742	0.0
9	4.8399383972640794E-5	0.0	0.0	1.203644280015604	0.0
10	4.8399383972640794E-5	0.0	0.0	1.4348965366368818	0.0
11	4.8399383972640794E-5	0.0	0.0	1.6196369852604515	0.0
12	4.8399383972640794E-5	0.0	0.0	1.7503153219865817	0.0
13	4.8399383972640794E-5	0.0	0.0	1.8400961792558304	0.0
14	4.8399383972640794E-5	0.0	0.0	1.9072745242098559	0.0
15	4.8399383972640794E-5	0.0	0.0	1.9589166669086635	0.0
16	4.8399383972640794E-5	0.0	0.0	2.0289021761331023	0.0
17	4.8399383972640794E-5	0.0	0.0	2.1042116175945313	0.0
18	4.8399383972640794E-5	0.0	0.0	2.199268007716798	0.0
19	4.8399383972640794E-5	0.0	0.0	2.2738030590346647	0.0
20	4.8399383972640794E-5	0.0	0.0	2.3465473331455438	0.0
21	9.679876794528159E-5	0.0	0.0	2.4233571555101245	0.0
22	9.679876794528159E-5	0.0	0.0	2.52480226431678	0.0
23	9.679876794528159E-5	0.0	0.0	2.640670389547282	0.0
24	9.679876794528159E-5	0.0	0.0	2.733452008622834	0.0
25	9.679876794528159E-5	0.0	0.0	2.8217808843729038	0.0
26	9.679876794528159E-5	0.0	0.0	2.9131105219292768	0.0
27	9.679876794528159E-5	0.0	0.0	3.0106836800181207	0.0
28	9.679876794528159E-5	0.0	0.0	3.1106284079216238	0.0
29	9.679876794528159E-5	0.0	0.0	3.2206402076914364	0.0
30	9.679876794528159E-5	0.0	0.0	3.366806347288812	0.0
31	9.679876794528159E-5	0.0	0.0	3.4882404016761677	0.0
32	9.679876794528159E-5	0.0	0.0	3.6220162989765465	0.0
33	9.679876794528159E-5	0.0	0.0	3.744176344123492	0.0
34	9.679876794528159E-5	0.0	0.0	3.882792179821135	0.0
35	9.679876794528159E-5	0.0	0.0	4.025763960076316	0.0
36	9.679876794528159E-5	0.0	0.0	4.171494505217938	0.0
37	9.679876794528159E-5	0.0	0.0	4.329179698200801	0.0
38	9.679876794528159E-5	0.0	0.0	4.51096778440204	0.0
39	9.679876794528159E-5	0.0	0.0	4.7795359660662236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGC	20	7.034596E-4	45.0	29
CGTTTTT	14615	0.0	42.61375	1
CGACGGT	75	0.0	39.000004	28
GGCGATA	670	0.0	38.955223	8
TAGGGAT	3825	0.0	38.705883	5
TACGGGA	905	0.0	38.53591	4
GCGATCG	205	0.0	38.414635	9
CACGACC	335	0.0	38.28358	27
TAAGGGA	3450	0.0	38.086956	4
TAGGGAC	2410	0.0	37.904564	5
TCGCGTA	30	1.14023715E-4	37.499996	30
TTAGGGA	3315	0.0	37.398193	4
AGGGCGA	1755	0.0	37.30769	6
GGGCGAT	3460	0.0	37.131504	7
AAGGGAT	4200	0.0	37.125	5
CACGGGA	535	0.0	37.009346	4
TAGGGCG	610	0.0	36.885242	5
ACGGGAT	1155	0.0	36.818184	5
AGGGATT	4520	0.0	36.686947	6
ACGGGAC	695	0.0	36.58273	5
>>END_MODULE