FastQCFastQC Report
Thu 26 May 2016
SRR1547966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547966_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1952187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65970.3379286922820406No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29790.15259808614646037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA1650.043.63636431
CGTAAGG3100.042.0967752
TATAGCG1450.041.8965531
GCGATAT2350.040.2127659
GGGCGAT32350.039.505417
CGTACGT403.4598816E-739.37541
AGGGCGA16850.038.8575676
CGTTTTT45900.038.676471
TATGGGC7050.038.617024
ATAGCGG4400.038.3522722
ATAACGC659.094947E-1238.07692711
TAGGGAT26100.037.758625
GGCGATA7550.037.5496678
TATTGCG900.037.5000041
ATGGGCG5300.037.358495
ACGGGTA1700.037.0588235
ACGGGAT6400.036.9140625
ATATACG552.752131E-936.818181
TAGGGCG7700.036.818185
TGGGCGA10450.036.818186
Produced by FastQC (version 0.11.5)