##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547966_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1952187
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.845095270074026	31.0	31.0	31.0	30.0	33.0
2	31.192176774048797	31.0	31.0	33.0	30.0	34.0
3	31.369608034476204	31.0	31.0	34.0	30.0	34.0
4	35.25386092623299	37.0	35.0	37.0	33.0	37.0
5	35.070648457345534	35.0	35.0	37.0	32.0	37.0
6	35.10011950699395	37.0	35.0	37.0	32.0	37.0
7	35.49827245033391	35.0	35.0	37.0	35.0	37.0
8	35.57562159772604	37.0	35.0	37.0	35.0	37.0
9	37.38486272063076	39.0	37.0	39.0	35.0	39.0
10	36.495772689808916	38.0	35.0	39.0	32.0	39.0
11	36.09686367135935	38.0	35.0	39.0	31.0	39.0
12	35.47235638798947	37.0	35.0	39.0	30.0	39.0
13	35.14096958949117	37.0	34.0	39.0	29.0	39.0
14	36.06513003108821	38.0	34.0	40.0	29.0	41.0
15	36.336427299229015	38.0	35.0	40.0	30.0	41.0
16	36.48142775256674	38.0	35.0	40.0	31.0	41.0
17	36.42848251729983	38.0	35.0	40.0	31.0	41.0
18	36.34195853163657	38.0	35.0	40.0	30.0	41.0
19	36.2798533132328	38.0	35.0	40.0	30.0	41.0
20	36.14483602236876	38.0	34.0	40.0	30.0	41.0
21	36.02369854937053	38.0	34.0	40.0	30.0	41.0
22	35.88859981139102	38.0	34.0	40.0	29.0	41.0
23	35.82681577123503	38.0	34.0	40.0	29.0	41.0
24	35.71094674844162	37.0	34.0	40.0	29.0	41.0
25	35.52698383915065	37.0	34.0	40.0	29.0	41.0
26	35.26941015384284	37.0	34.0	40.0	27.0	41.0
27	35.37340224066649	37.0	34.0	40.0	28.0	41.0
28	35.392688815159616	37.0	34.0	40.0	28.0	41.0
29	35.381589468631844	37.0	34.0	40.0	28.0	41.0
30	35.292995496845336	37.0	34.0	40.0	27.0	41.0
31	35.16640618957098	37.0	34.0	40.0	27.0	41.0
32	35.04321409782977	37.0	34.0	40.0	27.0	41.0
33	34.918291126823405	37.0	33.0	40.0	26.0	41.0
34	34.747682983238796	37.0	33.0	40.0	25.0	41.0
35	34.61453539030841	37.0	33.0	40.0	24.0	41.0
36	34.51042804813268	37.0	33.0	40.0	24.0	41.0
37	34.442693758333604	37.0	33.0	40.0	24.0	41.0
38	34.370217094981165	37.0	33.0	40.0	24.0	41.0
39	34.30604854965226	36.0	33.0	40.0	23.0	41.0
40	34.11432203984557	36.0	33.0	40.0	23.0	41.0
41	34.04846871739234	36.0	33.0	40.0	23.0	41.0
42	34.003881800257865	36.0	33.0	40.0	23.0	41.0
43	33.94308229693159	36.0	33.0	40.0	23.0	41.0
44	33.79717209468151	36.0	33.0	40.0	23.0	41.0
45	33.6729524374458	35.0	32.0	40.0	23.0	41.0
46	33.61552914756629	35.0	32.0	40.0	23.0	41.0
47	33.54111055959291	35.0	32.0	40.0	23.0	41.0
48	33.433761724670845	35.0	32.0	39.0	22.0	41.0
49	33.29769074376584	35.0	32.0	39.0	22.0	41.0
50	33.192076886077	35.0	32.0	39.0	21.0	41.0
51	32.85363646003175	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	5.0
13	11.0
14	38.0
15	141.0
16	327.0
17	771.0
18	1672.0
19	2961.0
20	4879.0
21	7440.0
22	11111.0
23	15493.0
24	21520.0
25	28517.0
26	35779.0
27	42280.0
28	48388.0
29	56732.0
30	67225.0
31	80615.0
32	97428.0
33	121733.0
34	181843.0
35	205034.0
36	161551.0
37	197573.0
38	258777.0
39	302307.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.11757531425012	25.165365817926254	29.349903467239564	12.367155400584062
2	30.571302851622306	26.525225298601008	28.83530112637775	14.068170723398937
3	28.922280498743202	26.71383427919559	30.489855736156425	13.874029485904781
4	25.811051912547313	29.10043966074971	29.94298189671379	15.145526529989187
5	23.391918909407757	33.48429223225029	28.79309205521807	14.330696803123882
6	22.099266105142593	42.617228779824885	25.271554415637436	10.01195069939509
7	90.66493117718744	3.3692469010397055	4.7677297308096	1.1980921909632634
8	91.17902127204003	2.7799590920337036	4.540855973326326	1.5001636625999455
9	86.31698705093314	5.578307815798384	5.9791403180125675	2.1255648152559155
10	44.85922711297637	31.2679574241607	12.501671202605078	11.371144260257854
11	37.051727114256984	25.140316988075423	22.128976373677318	15.678979523990275
12	34.60959426530348	22.980636588605496	25.374259740485925	17.035509405605097
13	25.458729107406207	30.128466176652136	26.654618640529826	17.75818607541183
14	19.997674403118143	33.525937832799826	27.916280561237215	18.560107202844808
15	18.97728035275309	26.811519593153733	37.26589717071162	16.94530288338156
16	22.824555229596342	24.60404664102363	34.96816647175706	17.603231657622963
17	23.323277944172357	24.23225848753219	26.859004798208368	25.585458770087087
18	24.543704061137586	25.993770064035875	29.872599295047042	19.589926579779497
19	27.6307034110974	27.25773709178475	25.467744637168465	19.64381485994938
20	29.69546462505897	27.476619811524206	24.9247638673959	17.903151696020924
21	26.561441091452814	27.4009610759625	27.5604232586325	18.477174573952187
22	25.73523950318284	24.421635837140602	27.48107635180441	22.362048307872147
23	22.670420405422227	28.6523780764855	26.435479797785767	22.241721720306508
24	23.008605220708876	26.967959524369334	31.042722853906923	18.980712401014863
25	21.880690733008674	27.413101306380998	29.773889489070466	20.93231847153987
26	20.89543675887607	32.14896933541715	26.00821540149586	20.947378504210917
27	21.07047122022634	31.290188900960818	27.96443168610384	19.674908192708997
28	19.41130639636469	29.724662647584477	31.7384041590278	19.12562679702303
29	21.143722399544714	27.487223303915044	30.604086596212348	20.764967700327887
30	22.381206308616953	29.05423507071812	28.635115385974807	19.92944323469012
31	26.342865719318898	27.742782837914604	26.032034840924563	19.882316601841936
32	27.514064994798144	27.78458211226691	25.905919873454746	18.795433019480203
33	26.68356054005072	28.091673594793942	25.246351911983844	19.978413953171493
34	21.459163492022025	28.847134009190718	27.99552501886346	21.698177479923796
35	21.80083158017137	28.568728303179974	28.607607775279725	21.022832341368936
36	26.778684623962768	27.958182284791366	25.281287089812604	19.981846001433265
37	22.266514427152725	30.909999912918178	27.376424492120886	19.447061167808208
38	22.897960082717486	31.333576137941705	24.302077618588793	21.46638616075202
39	22.301091032775037	29.235109136573495	26.784985249876165	21.678814580775306
40	24.184158587266484	27.181719784016593	26.301168894168438	22.332952734548485
41	20.54900478284099	26.718393268677644	26.993162028022933	25.73943992045844
42	22.419112513299186	27.121018631924095	26.198668467723635	24.261200387053083
43	22.307699006293966	27.331961538520645	26.755889676552503	23.604449778632887
44	21.0678075409784	28.909371899310877	27.78068904259684	22.242131517113883
45	20.840165414481294	31.127397119230892	25.17094929942675	22.86148816686106
46	22.848118546020437	29.977660951537942	25.723970090980014	21.450250411461607
47	21.817172227865466	28.80840821089373	27.166967098951073	22.20745246228973
48	22.82978013889038	27.052992361899758	29.383558030045275	20.733669469164585
49	22.62032274572057	26.38333315404723	28.954142200516653	22.04220189971555
50	20.964128948712393	29.978890341960067	27.2627571026751	21.794223606652434
51	20.279614606592503	30.395961042666507	26.130795871502066	23.193628479238924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	966.0
1	1560.0
2	2154.0
3	7215.5
4	12277.0
5	9659.0
6	7041.0
7	6827.0
8	6613.0
9	7296.5
10	7980.0
11	8365.0
12	8750.0
13	9025.5
14	9301.0
15	9114.5
16	8928.0
17	8969.5
18	9011.0
19	8889.0
20	8767.0
21	9415.5
22	10064.0
23	10699.0
24	11334.0
25	13573.5
26	18698.5
27	21584.0
28	24358.0
29	27132.0
30	32912.5
31	38693.0
32	44326.5
33	49960.0
34	54406.0
35	58852.0
36	63380.5
37	67909.0
38	74445.5
39	80982.0
40	98714.5
41	116447.0
42	127810.5
43	139174.0
44	143709.0
45	148244.0
46	148829.0
47	149414.0
48	145027.0
49	140640.0
50	134190.0
51	127740.0
52	121544.0
53	115348.0
54	107131.5
55	98915.0
56	96095.5
57	93276.0
58	87428.0
59	81580.0
60	79187.5
61	76795.0
62	69153.0
63	61511.0
64	53655.0
65	45799.0
66	38532.0
67	31265.0
68	27136.5
69	23008.0
70	18764.0
71	14520.0
72	12226.5
73	9933.0
74	8322.5
75	5210.0
76	3708.0
77	2758.5
78	1809.0
79	1419.0
80	1029.0
81	871.0
82	713.0
83	484.5
84	256.0
85	186.5
86	117.0
87	88.0
88	59.0
89	48.0
90	37.0
91	27.0
92	17.0
93	13.0
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1952187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.935805477818178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4835385358661	17.43535440011424
2	6.899894960662234	3.0270950734892934
3	2.471101691293316	1.6261681804835302
4	1.28329868749117	1.1260076151498273
5	0.8551210866605972	0.9378884908483681
6	0.5809917546561916	0.7646713268612689
7	0.4794127280799239	0.7361413042725947
8	0.38261613226699975	0.6714394440067247
9	0.3288657545476818	0.6492541698066462
>10	4.611106082194468	25.853924573071634
>50	2.150790251444765	33.499596734296766
>100	0.47135401901582863	12.981133444488599
>500	0.0014312368655137984	0.19025572085603296
>1k	2.3853947758563306E-4	0.15587783070135372
>5k	2.3853947758563306E-4	0.3451916915531489
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6597	0.3379286922820406	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2979	0.15259808614646037	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017877385721757188	0.0
2	0.0	0.0	0.0	0.06741157481327352	0.0
3	0.0	0.0	0.0	0.10562512710104104	0.0
4	0.0	0.0	0.0	0.1909653122369937	0.0
5	0.0	0.0	0.0	0.342743804768703	0.0
6	0.0	0.0	0.0	0.5922076112585526	0.0
7	0.0	0.0	0.0	0.7082313323467475	0.0
8	0.0	0.0	0.0	0.991349701642312	0.0
9	0.0	0.0	0.0	1.1052219894917854	0.0
10	0.0	0.0	0.0	1.2997217991923928	0.0
11	0.0	0.0	0.0	1.5461633542278481	0.0
12	0.0	0.0	0.0	1.7286766073127215	0.0
13	0.0	0.0	0.0	1.8260545736653302	0.0
14	0.0	0.0	0.0	1.8720542652932326	0.0
15	0.0	0.0	0.0	1.9318333745691372	0.0
16	0.0	0.0	0.0	2.0516477161255557	0.0
17	0.0	0.0	0.0	2.1906712830277018	0.0
18	0.0	0.0	0.0	2.3523873481382673	0.0
19	0.0	0.0	0.0	2.4537096087618657	0.0
20	0.0	0.0	0.0	2.5621008643126912	0.0
21	0.0	0.0	0.0	2.70409545806831	0.0
22	0.0	0.0	0.0	2.860176817077462	0.0
23	0.0	0.0	0.0	3.032957395987167	0.0
24	0.0	0.0	0.0	3.167882994815558	0.0
25	0.0	0.0	0.0	3.282933448486236	0.0
26	0.0	0.0	0.0	3.3995206401845723	0.0
27	0.0	0.0	0.0	3.524508666434107	0.0
28	0.0	0.0	0.0	3.6511871045140656	0.0
29	0.0	0.0	0.0	3.793437821274294	0.0
30	0.0	0.0	0.0	3.967908812014423	0.0
31	0.0	0.0	0.0	4.121787513183932	0.0
32	0.0	0.0	0.0	4.271056000270466	0.0
33	0.0	0.0	0.0	4.426830011674086	0.0
34	0.0	0.0	0.0	4.588546076784652	0.0
35	0.0	0.0	0.0	4.768241976818819	0.0
36	0.0	0.0	0.0	4.93615621864094	0.0
37	0.0	0.0	0.0	5.122767439799568	0.0
38	0.0	0.0	0.0	5.336732597850514	0.0
39	0.0	0.0	0.0	5.6364989624457085	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	165	0.0	43.636364	31
CGTAAGG	310	0.0	42.096775	2
TATAGCG	145	0.0	41.896553	1
GCGATAT	235	0.0	40.212765	9
GGGCGAT	3235	0.0	39.50541	7
CGTACGT	40	3.4598816E-7	39.375	41
AGGGCGA	1685	0.0	38.857567	6
CGTTTTT	4590	0.0	38.67647	1
TATGGGC	705	0.0	38.61702	4
ATAGCGG	440	0.0	38.352272	2
ATAACGC	65	9.094947E-12	38.076927	11
TAGGGAT	2610	0.0	37.75862	5
GGCGATA	755	0.0	37.549667	8
TATTGCG	90	0.0	37.500004	1
ATGGGCG	530	0.0	37.35849	5
ACGGGTA	170	0.0	37.058823	5
ACGGGAT	640	0.0	36.914062	5
ATATACG	55	2.752131E-9	36.81818	1
TAGGGCG	770	0.0	36.81818	5
TGGGCGA	1045	0.0	36.81818	6
>>END_MODULE