FastQCFastQC Report
Thu 26 May 2016
SRR1547965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547965_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1285442
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275962.146810202249499No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.19005135976574594No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT24110.18756194367384915No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC19180.14920937700806414No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC19090.14850922873221817No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT16470.12812713447981317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCACG302.1657033E-644.9999961
CGTTTTT113300.043.2722851
AAATGCG603.6379788E-1241.2499961
TTGTACG556.184564E-1140.9090921
TGTTACG451.9286745E-840.01
ACGGGAT5750.039.521745
CGTTCGA403.4586446E-739.37514
GCCGACC403.4586446E-739.37514
TAGGGAT16850.038.99115
CTTGCGG1100.038.8636362
AGGGATC12050.038.8381736
AAGGGCG4200.038.571435
TAGGGAC14300.038.548955
GGCGATA3450.038.478268
TAGGGCG4100.038.4146355
CGGGATC3400.038.382356
TAAGGGA16700.038.1287424
AGGGCGA10550.037.962096
ATTAGCG950.037.8947371
CGGTTTT9400.037.579791