##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547965_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1285442
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.811305372004337	31.0	31.0	31.0	30.0	33.0
2	31.071799427745475	31.0	31.0	33.0	30.0	34.0
3	30.93009719613954	31.0	31.0	34.0	28.0	34.0
4	35.002241252425236	35.0	35.0	37.0	32.0	37.0
5	34.95955943558714	35.0	35.0	37.0	32.0	37.0
6	35.03997068712552	36.0	35.0	37.0	32.0	37.0
7	35.502625556034424	35.0	35.0	37.0	35.0	37.0
8	35.60044560548045	37.0	35.0	37.0	35.0	37.0
9	37.41210105162271	39.0	37.0	39.0	35.0	39.0
10	36.586001546549745	39.0	35.0	39.0	32.0	39.0
11	36.1532710149505	38.0	35.0	39.0	31.0	39.0
12	35.94257228253006	37.0	35.0	39.0	31.0	39.0
13	35.88906617334738	38.0	35.0	39.0	30.0	39.0
14	37.00383681255163	39.0	36.0	41.0	31.0	41.0
15	37.11291369038821	39.0	36.0	41.0	31.0	41.0
16	37.16806748184671	39.0	35.0	41.0	31.0	41.0
17	37.09110951719331	39.0	35.0	41.0	31.0	41.0
18	36.917618997978906	39.0	35.0	40.0	31.0	41.0
19	36.7607935636147	38.0	35.0	40.0	31.0	41.0
20	36.546307029021925	38.0	35.0	40.0	31.0	41.0
21	36.44289201690936	38.0	35.0	40.0	30.0	41.0
22	36.32243772959029	38.0	35.0	40.0	30.0	41.0
23	36.21882512007543	38.0	35.0	40.0	30.0	41.0
24	36.051500573343645	38.0	34.0	40.0	30.0	41.0
25	35.849861759612644	37.0	34.0	40.0	30.0	41.0
26	35.600704660342515	37.0	34.0	40.0	29.0	41.0
27	35.606432651181464	37.0	34.0	40.0	29.0	41.0
28	35.55703174472283	37.0	34.0	40.0	29.0	41.0
29	35.47270977609258	37.0	34.0	40.0	29.0	41.0
30	35.320546551302975	37.0	34.0	40.0	28.0	41.0
31	35.194475518926566	37.0	34.0	40.0	27.0	41.0
32	34.95352337950681	37.0	33.0	40.0	26.0	41.0
33	34.671110793019054	37.0	33.0	40.0	25.0	41.0
34	34.26024589207448	36.0	33.0	40.0	23.0	41.0
35	33.963468596793945	36.0	33.0	40.0	21.0	41.0
36	33.82560706745228	36.0	33.0	40.0	21.0	41.0
37	33.72294432576499	36.0	33.0	40.0	21.0	41.0
38	33.602248098319485	36.0	33.0	40.0	20.0	41.0
39	33.53548507050493	36.0	33.0	40.0	20.0	41.0
40	33.35444539699185	36.0	33.0	40.0	18.0	41.0
41	33.24198369121283	36.0	32.0	40.0	18.0	41.0
42	33.18383015336359	35.0	32.0	40.0	18.0	41.0
43	33.128810168020024	35.0	32.0	40.0	18.0	41.0
44	32.93004429604758	35.0	32.0	40.0	18.0	41.0
45	32.820715364831706	35.0	31.0	40.0	18.0	41.0
46	32.75040258525861	35.0	31.0	40.0	18.0	41.0
47	32.68958381630598	35.0	31.0	39.0	18.0	41.0
48	32.58268673343488	35.0	31.0	39.0	17.0	41.0
49	32.44390800985187	35.0	31.0	39.0	17.0	41.0
50	32.33012380177402	35.0	31.0	39.0	15.0	41.0
51	32.0656941347801	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	8.0
14	12.0
15	50.0
16	168.0
17	380.0
18	880.0
19	1657.0
20	2900.0
21	4635.0
22	7148.0
23	10787.0
24	16162.0
25	23758.0
26	31507.0
27	34537.0
28	34929.0
29	37678.0
30	43098.0
31	50673.0
32	60452.0
33	74663.0
34	100336.0
35	124613.0
36	137794.0
37	136619.0
38	163271.0
39	186705.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.061644165975594	23.86043088680781	26.64958823501955	19.42833671219705
2	35.50545259918378	25.23863387068417	26.577472962607413	12.678440567524635
3	26.660323841915854	25.43156361780617	35.20213280723673	12.705979733041243
4	23.175841461536187	27.12918980397404	35.92670847848444	13.768260256005327
5	21.8531057799574	30.882606916531437	33.84407853485416	13.420208768657007
6	19.8479589121874	39.169406320938634	30.82846211653268	10.154172650341284
7	83.16664618084675	2.9409339355645763	12.300671675579295	1.5917482080093852
8	83.2152675888916	2.905226373496432	11.958065785931998	1.9214402516799667
9	78.375375940727	5.467613474586951	13.33657994681985	2.8204306378661967
10	37.000191373862066	33.35879798544003	18.942900574277175	10.698110066420734
11	24.21338341208705	27.738474392465783	31.26652155445364	16.781620640993523
12	24.13232180059466	25.13306706953717	32.33152487626824	18.40308625359993
13	21.06419426158473	28.368218869462797	33.28598256475205	17.281604304200425
14	17.189573703053114	31.97164866248341	32.45358405902405	18.38519357543942
15	16.65123747318043	31.202419090087304	35.74949317044254	16.39685026628973
16	19.12322765243395	30.572907995848897	34.1259271130086	16.177937238708555
17	18.899413586921852	31.26037580847677	30.933873329173935	18.90633727542744
18	19.237351821396842	31.650513986628724	32.23778280155775	16.87435139041668
19	20.78226788917742	33.39979555670345	28.77842796485567	17.03950858926346
20	22.26603767420078	32.70089198890343	29.044406515424264	15.988663821471524
21	20.956915986874552	32.44315962913924	30.472086644127078	16.12783773985913
22	20.596961978836852	30.398259898151764	30.40673947171479	18.598038651296598
23	18.210623271995157	33.402829532565455	30.24726125332765	18.13928594211174
24	17.966271523724913	32.769350931430594	32.44720493028857	16.81717261455593
25	18.653116982329813	34.35293074288844	29.893452991266816	17.10049928351493
26	18.084363199584267	35.66314154975487	28.8778490200258	17.374646230635065
27	17.468466099598427	34.94766780609316	29.892130488968	17.69173560534042
28	16.835298675475048	34.777531775062585	31.491580328011686	16.895589221450678
29	17.70939490074231	32.64394659580129	31.29919514065979	18.34746336279661
30	17.904891858209083	34.12888329461773	30.87716131883041	17.08906352834278
31	19.09179877427375	34.84777998540579	28.37833212233613	17.68208911798432
32	19.16088007082389	34.50431835897691	28.44889150969083	17.88591006050837
33	18.384571221416447	35.4026085968873	27.81689099936053	18.395929182335728
34	17.051722286964328	35.20547796011022	28.548545947619573	19.19425380530588
35	17.386548751324447	35.55461856699875	27.90713233269179	19.151700348985017
36	18.56528727083758	36.278649678476356	26.100594192503436	19.055468858182635
37	17.706283130627444	36.54416146352772	26.91650031662261	18.833055089222228
38	17.50448483867806	36.70472880145506	26.755388418925165	19.035397940941714
39	17.973039623724755	36.10687996813547	26.31281691433764	19.607263493802133
40	19.68326847885786	35.14775462447936	26.93540432007045	18.233572576592334
41	17.355353255922864	35.22617123137411	27.49241117063236	19.926064342070664
42	18.49783965359775	35.87972074974989	26.5163266798502	19.10611291680216
43	18.69994912255862	35.497906556655224	27.14241482696224	18.659729493823914
44	18.62347737198567	36.11458159916978	26.333743568360145	18.92819746048441
45	17.375346378910912	36.49546226122999	26.030579364918836	20.098611994940264
46	18.74413625818979	36.151222692272384	25.947961868369013	19.156679181168812
47	18.094009686940367	35.82036373480873	27.29092405569446	18.794702522556445
48	18.067248463952478	35.309566670452654	27.566237916607673	19.056946948987196
49	17.80834919039521	34.891111384255375	27.555268926952753	19.74527049839666
50	17.125393444433897	35.354687337118285	28.6215169568133	18.89840226163452
51	16.947633576621893	35.59639408079089	27.381865537301564	20.074106805285652
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	947.0
1	1266.0
2	1585.0
3	19827.0
4	38069.0
5	27270.0
6	16471.0
7	16056.5
8	15642.0
9	19066.5
10	22491.0
11	26336.5
12	30182.0
13	31194.5
14	32207.0
15	30062.5
16	27918.0
17	25312.0
18	22706.0
19	20172.0
20	17638.0
21	16501.0
22	15364.0
23	15003.0
24	14642.0
25	15012.5
26	16696.5
27	18010.0
28	19842.5
29	21675.0
30	24086.0
31	26497.0
32	29806.0
33	33115.0
34	35684.5
35	38254.0
36	40672.0
37	43090.0
38	48119.0
39	53148.0
40	56649.0
41	60150.0
42	65596.0
43	71042.0
44	75264.5
45	79487.0
46	88207.0
47	96927.0
48	92789.5
49	88652.0
50	84057.0
51	79462.0
52	71994.0
53	64526.0
54	58848.5
55	53171.0
56	48653.0
57	44135.0
58	40926.5
59	37718.0
60	34333.0
61	30948.0
62	27058.5
63	23169.0
64	19878.0
65	16587.0
66	14190.0
67	11793.0
68	9844.5
69	7896.0
70	6638.0
71	5380.0
72	4601.0
73	3822.0
74	3157.5
75	2013.0
76	1533.0
77	1178.0
78	823.0
79	571.0
80	319.0
81	254.0
82	189.0
83	143.5
84	98.0
85	66.0
86	34.0
87	31.0
88	28.0
89	19.5
90	11.0
91	6.5
92	2.0
93	5.0
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1285442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.86008436324975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88832800092904	25.062013381197843
2	9.910965598879637	7.108161249941797
3	4.82105416451658	5.186502271780833
4	2.8583367474277126	4.100007876053387
5	1.8940563614809103	3.3960510455727655
6	1.4209240110941854	3.0572672946962824
7	1.1383910662502645	2.8575959771873025
8	0.9487111417945665	2.7216689264886518
9	0.8051904017351755	2.598677616123212
>10	6.257333541987009	38.49401747450608
>50	0.03928476787469754	0.8882351373017497
>100	0.013895246063354544	0.9310012936417864
>500	0.0017644749834205004	0.429157409102819
>1k	0.001543915610492938	0.9869987667147216
>5k	0.0	0.0
>10k+	2.2055937292756256E-4	2.1826442796906367
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27596	2.146810202249499	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2443	0.19005135976574594	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	2411	0.18756194367384915	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	1918	0.14920937700806414	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	1909	0.14850922873221817	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	1647	0.12812713447981317	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05531171379183192	0.0
2	0.0	0.0	0.0	0.18810650344395158	0.0
3	0.0	0.0	0.0	0.3352932298773496	0.0
4	0.0	0.0	0.0	0.521377082746635	0.0
5	0.0	0.0	0.0	1.0982992620437173	0.0
6	0.0	0.0	0.0	1.9506908907597542	0.0
7	0.0	0.0	0.0	2.5342255815509374	0.0
8	0.0	0.0	0.0	3.289218805671512	0.0
9	0.0	0.0	0.0	3.7402698838220627	0.0
10	0.0	0.0	0.0	4.266470210246748	0.0
11	0.0	0.0	0.0	4.620589649319067	0.0
12	0.0	0.0	0.0	4.93892373207037	0.0
13	0.0	0.0	0.0	5.13006421137632	0.0
14	0.0	0.0	0.0	5.254379427465417	0.0
15	0.0	0.0	0.0	5.364147118267491	0.0
16	0.0	0.0	0.0	5.511567227459504	0.0
17	0.0	0.0	0.0	5.668400441249002	0.0
18	0.0	0.0	0.0	5.869342996416797	0.0
19	0.0	0.0	0.0	5.991479973425483	0.0
20	0.0	0.0	0.0	6.122252112502936	0.0
21	0.0	0.0	0.0	6.258858820545774	0.0
22	0.0	0.0	0.0	6.409001728588299	0.0
23	0.0	0.0	0.0	6.58349423777969	0.0
24	0.0	0.0	0.0	6.727413605592473	0.0
25	0.0	0.0	0.0	6.862231045819259	0.0
26	0.0	0.0	0.0	6.9916806825978925	0.0
27	0.0	0.0	0.0	7.127276065353396	0.0
28	0.0	0.0	0.0	7.266916749258232	0.0
29	0.0	0.0	0.0	7.414259064197373	0.0
30	0.0	0.0	0.0	7.580583176837228	0.0
31	0.0	0.0	0.0	7.735704917063547	0.0
32	0.0	0.0	0.0	7.905918742346991	0.0
33	0.0	0.0	0.0	8.063296515906591	0.0
34	0.0	0.0	0.0	8.217718107857063	0.0
35	0.0	0.0	0.0	8.388632081416354	0.0
36	0.0	0.0	0.0	8.547954711297749	0.0
37	0.0	0.0	0.0	8.723069574512113	0.0
38	0.0	0.0	0.0	8.897250906692017	0.0
39	0.0	0.0	0.0	9.094770514733453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	30	2.1657033E-6	44.999996	1
CGTTTTT	11330	0.0	43.272285	1
AAATGCG	60	3.6379788E-12	41.249996	1
TTGTACG	55	6.184564E-11	40.909092	1
TGTTACG	45	1.9286745E-8	40.0	1
ACGGGAT	575	0.0	39.52174	5
CGTTCGA	40	3.4586446E-7	39.375	14
GCCGACC	40	3.4586446E-7	39.375	14
TAGGGAT	1685	0.0	38.9911	5
CTTGCGG	110	0.0	38.863636	2
AGGGATC	1205	0.0	38.838173	6
AAGGGCG	420	0.0	38.57143	5
TAGGGAC	1430	0.0	38.54895	5
GGCGATA	345	0.0	38.47826	8
TAGGGCG	410	0.0	38.414635	5
CGGGATC	340	0.0	38.38235	6
TAAGGGA	1670	0.0	38.128742	4
AGGGCGA	1055	0.0	37.96209	6
ATTAGCG	95	0.0	37.894737	1
CGGTTTT	940	0.0	37.57979	1
>>END_MODULE