FastQCFastQC Report
Thu 26 May 2016
SRR1547964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547964_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2255174
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107080.47481923789472563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG900.042.5000041
CGTTTTT78900.040.380231
GCGATCG1650.039.5454569
GGGCGAT40350.039.4795537
TATAGCG1150.039.1304361
ACGGGAT9300.038.709685
TCGATAC356.2509644E-638.57142625
CACGACC9600.038.2031227
AGGGCGA22600.038.130536
TAAGGGA31300.037.7396164
GGTACGC301.1402703E-437.4999968
GCGAATA301.1402703E-437.49999612
GGCGATA9850.037.461938
AGGGATC22950.037.3529436
CGTAAGG3550.036.7605632
GACCGAT12150.036.481489
TAGGGAC25000.036.4499975
AAGGGAT33450.036.3901335
GGCGATT11150.036.3228728
AACGGGA8450.036.2130174
Produced by FastQC (version 0.11.5)