##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547964_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2255174
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979019800689436	31.0	31.0	33.0	30.0	33.0
2	31.32533498523839	31.0	31.0	33.0	30.0	34.0
3	31.454586209312453	31.0	31.0	34.0	30.0	34.0
4	35.38744371831176	37.0	35.0	37.0	33.0	37.0
5	35.28026928299102	37.0	35.0	37.0	33.0	37.0
6	35.329781648777434	37.0	35.0	37.0	33.0	37.0
7	35.576817575938705	36.0	35.0	37.0	35.0	37.0
8	35.65077240159739	37.0	35.0	37.0	35.0	37.0
9	37.50176083974008	39.0	37.0	39.0	35.0	39.0
10	36.73021726926614	39.0	37.0	39.0	32.0	39.0
11	36.40384333980438	38.0	35.0	39.0	32.0	39.0
12	35.96008467639304	37.0	35.0	39.0	31.0	39.0
13	35.78298215570062	37.0	35.0	39.0	30.0	39.0
14	36.82629633012797	39.0	35.0	40.0	31.0	41.0
15	36.972391043883974	39.0	35.0	40.0	31.0	41.0
16	37.074651445963816	39.0	35.0	41.0	32.0	41.0
17	36.997103549437874	39.0	35.0	40.0	31.0	41.0
18	36.88955974128825	38.0	35.0	40.0	31.0	41.0
19	36.82184212836792	38.0	35.0	40.0	31.0	41.0
20	36.6917107061362	38.0	35.0	40.0	31.0	41.0
21	36.57208002575411	38.0	35.0	40.0	31.0	41.0
22	36.46153157139981	38.0	35.0	40.0	30.0	41.0
23	36.36622806045121	38.0	35.0	40.0	30.0	41.0
24	36.25530136477274	38.0	35.0	40.0	30.0	41.0
25	36.089961572809905	38.0	34.0	40.0	30.0	41.0
26	35.8808349156207	38.0	34.0	40.0	30.0	41.0
27	35.969492376198026	38.0	34.0	40.0	30.0	41.0
28	35.98404025587383	38.0	34.0	40.0	30.0	41.0
29	35.96294299242542	38.0	34.0	40.0	30.0	41.0
30	35.88907773856918	38.0	34.0	40.0	30.0	41.0
31	35.80311585713564	38.0	34.0	40.0	29.0	41.0
32	35.64929801425522	38.0	34.0	40.0	29.0	41.0
33	35.482477627003504	38.0	34.0	40.0	28.0	41.0
34	35.275180540392896	38.0	34.0	40.0	27.0	41.0
35	35.08367159252457	38.0	34.0	40.0	25.0	41.0
36	35.01547375058421	38.0	34.0	40.0	25.0	41.0
37	34.96352121831841	38.0	34.0	40.0	25.0	41.0
38	34.8778630828486	38.0	34.0	40.0	25.0	41.0
39	34.8286495853535	38.0	34.0	40.0	24.0	41.0
40	34.64931353412198	37.0	33.0	40.0	24.0	41.0
41	34.57180687609914	37.0	33.0	40.0	24.0	41.0
42	34.52921504061327	37.0	33.0	40.0	24.0	41.0
43	34.4803416499126	37.0	33.0	40.0	23.0	41.0
44	34.328712108245306	37.0	33.0	40.0	23.0	41.0
45	34.205845313931434	37.0	33.0	40.0	23.0	41.0
46	34.13465745880362	37.0	33.0	40.0	23.0	41.0
47	34.08841934147875	36.0	33.0	40.0	23.0	41.0
48	33.99266442411982	36.0	33.0	40.0	23.0	41.0
49	33.8530379474045	36.0	33.0	40.0	23.0	41.0
50	33.737672126407986	36.0	33.0	40.0	23.0	41.0
51	33.41771677041328	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	5.0
13	10.0
14	38.0
15	104.0
16	221.0
17	558.0
18	1212.0
19	2292.0
20	3941.0
21	6261.0
22	9478.0
23	14003.0
24	20315.0
25	28943.0
26	37820.0
27	43291.0
28	47811.0
29	55529.0
30	66096.0
31	80441.0
32	99562.0
33	128795.0
34	191539.0
35	219447.0
36	201748.0
37	250731.0
38	341732.0
39	403202.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.62113078636061	24.772279212158352	28.233342527006783	13.373247474474253
2	32.638723220469906	25.732870279632525	27.946801444145773	13.681605055751794
3	29.650572417028577	26.169909727586433	30.832742839355188	13.346775016029804
4	25.94553679671724	28.357058036320037	30.738204679550226	14.959200487412502
5	23.551708205220528	33.00312969198829	29.115181356294457	14.329980746496723
6	22.327855855024932	41.929669284942094	25.73278159467961	10.009693265353361
7	89.89794135618804	2.952676822276241	5.9587419862059425	1.1906398353297794
8	90.44481711832435	2.520869786544187	5.754278827265657	1.2800342678658054
9	85.42400719412338	5.283627782157829	7.146366533136689	2.1459984905821012
10	39.253423460894815	35.50519826851498	13.834808311908528	11.40656995868168
11	33.56348556696734	25.677530869014987	24.657166143277635	16.10181742074004
12	31.518100155464722	23.45712570293911	27.327204020621025	17.697570120975143
13	24.971332588970963	28.18997558503246	28.323535124119026	18.51515670187755
14	20.90969477299756	30.439203360804978	29.032482637703342	19.618619228494122
15	20.37736334313893	27.056493201854938	34.89282866865262	17.673314786353515
16	24.547595883953967	25.464953036883188	32.69264367184084	17.294807407322008
17	23.78831965959168	25.3465586247447	28.219818071687598	22.645303643976032
18	24.88455436254586	26.10228745099048	30.05005378742394	18.963104399039718
19	27.220649049696387	27.042347951865352	26.64229012927606	19.094712869162205
20	29.026629430811102	27.067224081157377	26.32515273766015	17.58099375037137
21	26.587926253140555	26.96572415254876	28.411687967314275	18.03466162699641
22	25.654871863545786	24.406542466346277	28.55673220780303	21.381853462304903
23	23.206945450772313	28.54604567097705	28.158448084271992	20.088560793978647
24	22.979424204074718	26.718647873733914	31.159502548362124	19.142425373829248
25	22.888256072480438	27.213022143745892	29.67841949224317	20.220302291530498
26	22.401065283654386	30.273672896193375	27.072944260620247	20.252317559531992
27	21.593189705095924	29.512445602867004	28.743369691207864	20.150995000829205
28	19.8923896781357	29.664540297112328	30.906573062655035	19.53649696209694
29	21.27317005251036	27.633122765693468	30.802900352700057	20.290806829096113
30	22.326525580731243	28.021873256786396	29.985535484179938	19.666065678302427
31	24.98596560620156	27.833151676988116	27.65830929232068	19.52257342448964
32	25.652299999911314	27.623722160684714	27.645139576813143	19.078838262590825
33	24.91993965875804	28.227001552873528	27.007406080417738	19.845652707950695
34	22.020917232993995	28.83453782280214	29.03066459616863	20.11388034803523
35	21.86580725034964	28.584978365305737	29.318092528558772	20.23112185578585
36	24.909652204220162	29.467748386598995	26.364440171800492	19.258159237380355
37	22.846529802135002	31.129615719230536	26.552940039216487	19.470914439417978
38	23.34569305960427	30.14525708437575	25.958795197177693	20.55025465884229
39	22.929139835773206	29.660106049466695	26.521279511026645	20.88947460373346
40	24.32437585747264	27.703627303259083	26.738247248327625	21.233749590940654
41	21.010839961794524	27.844370323531574	27.663719074448355	23.481070640225543
42	22.850254570157336	27.87305990579884	26.590453774298567	22.686231749745254
43	22.49649029298848	27.765485057915708	27.14100996198076	22.597014687115053
44	21.769584076439337	29.099838859440556	27.491359868462478	21.639217195657633
45	21.337599670801456	30.364264575593726	26.200683406247144	22.097452347357677
46	22.29060817480159	29.832376570499658	26.580432374619427	21.29658288007932
47	21.83702898312946	29.27800692984222	27.662743539966318	21.222220547062
48	22.87349002782047	27.738702202136068	28.46294787009783	20.924859899945638
49	22.44589552735177	27.9738060123077	28.378741507307197	21.201556953033336
50	21.59957502170564	29.935517170737157	27.599599853492457	20.86530795406474
51	21.064050933542156	29.759654909111227	27.072944260620247	22.103349896726375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	790.0
1	1461.5
2	2133.0
3	11278.5
4	20424.0
5	16023.5
6	11623.0
7	11132.5
8	10642.0
9	11113.0
10	11584.0
11	12137.5
12	12691.0
13	12633.5
14	12576.0
15	12201.5
16	11827.0
17	11323.5
18	10820.0
19	10737.0
20	10654.0
21	10930.5
22	11207.0
23	12628.0
24	14049.0
25	15434.5
26	21283.5
27	25747.0
28	30592.5
29	35438.0
30	40958.5
31	46479.0
32	52228.0
33	57977.0
34	65369.0
35	72761.0
36	76736.0
37	80711.0
38	90727.5
39	100744.0
40	112907.5
41	125071.0
42	137178.5
43	149286.0
44	155401.0
45	161516.0
46	162670.0
47	163824.0
48	161065.5
49	158307.0
50	157616.5
51	156926.0
52	147774.0
53	138622.0
54	130388.5
55	122155.0
56	113296.0
57	104437.0
58	101668.5
59	98900.0
60	92620.0
61	86340.0
62	76955.0
63	67570.0
64	57060.5
65	46551.0
66	39517.0
67	32483.0
68	27278.5
69	22074.0
70	18867.0
71	15660.0
72	13350.0
73	11040.0
74	9566.5
75	6333.0
76	4573.0
77	3345.5
78	2118.0
79	1494.5
80	871.0
81	685.0
82	499.0
83	409.5
84	320.0
85	219.5
86	119.0
87	89.0
88	59.0
89	46.0
90	33.0
91	25.0
92	17.0
93	13.5
94	10.0
95	5.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2255174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.887530234960163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91273195189575	15.85629483863649
2	6.684508448910089	2.7924574466491223
3	2.4702627088275095	1.5479306105678758
4	1.4182065366409722	1.1849132765402577
5	0.960918215883795	1.0035604143798373
6	0.7403469093240975	0.9278411071719839
7	0.5880777579118449	0.8598444364223872
8	0.5201341547892826	0.8691454307517271
9	0.44329871175832664	0.8333473720473845
>10	8.203862808689134	44.15281064069656
>50	1.9723340167010475	26.79365255707386
>100	0.08422676168142955	2.5234839460474583
>500	4.3640679467679537E-4	0.05305197788998204
>1k	4.3640679467679537E-4	0.11362421037777082
>5k	0.0	0.0
>10k+	2.1820339733839768E-4	0.4880417347473606
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10708	0.47481923789472563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012149838549043222	0.0
2	0.0	0.0	0.0	0.03817887222892779	0.0
3	0.0	0.0	0.0	0.06877518098381766	0.0
4	0.0	0.0	0.0	0.11493569897489063	0.0
5	0.0	0.0	0.0	0.20752278981577474	0.0
6	0.0	0.0	0.0	0.40187586412400994	0.0
7	0.0	0.0	0.0	0.5077656979018027	0.0
8	0.0	0.0	0.0	0.7235805308149171	0.0
9	0.0	0.0	0.0	0.847961177275013	0.0
10	0.0	0.0	0.0	1.017704177149967	0.0
11	0.0	0.0	0.0	1.193389068870074	0.0
12	0.0	0.0	0.0	1.3277024300563947	0.0
13	0.0	0.0	0.0	1.4123078751351337	0.0
14	0.0	0.0	0.0	1.4590448453201394	0.0
15	0.0	0.0	0.0	1.5087527614277214	0.0
16	0.0	0.0	0.0	1.587460657137764	0.0
17	0.0	0.0	0.0	1.6817771045604464	0.0
18	0.0	0.0	0.0	1.7866914038561992	0.0
19	0.0	0.0	0.0	1.8588366130507004	0.0
20	4.4342476456362126E-5	0.0	0.0	1.9335536858796705	0.0
21	4.4342476456362126E-5	0.0	0.0	2.031905298659882	0.0
22	4.4342476456362126E-5	0.0	0.0	2.1406330509308815	0.0
23	4.4342476456362126E-5	0.0	0.0	2.2572981064875703	0.0
24	4.4342476456362126E-5	0.0	0.0	2.354009047638896	0.0
25	4.4342476456362126E-5	0.0	0.0	2.440831616540453	0.0
26	4.4342476456362126E-5	0.0	0.0	2.528097610206574	0.0
27	4.4342476456362126E-5	0.0	0.0	2.6222366877234307	0.0
28	4.4342476456362126E-5	0.0	0.0	2.7136708741764495	0.0
29	4.4342476456362126E-5	0.0	0.0	2.822930736164926	0.0
30	4.4342476456362126E-5	0.0	0.0	2.9559138230575557	0.0
31	4.4342476456362126E-5	0.0	0.0	3.0713816317499227	0.0
32	4.4342476456362126E-5	0.0	0.0	3.190795920846906	0.0
33	4.4342476456362126E-5	0.0	0.0	3.308081771073984	0.0
34	4.4342476456362126E-5	0.0	0.0	3.4294027866585903	0.0
35	4.4342476456362126E-5	0.0	0.0	3.564070887656562	0.0
36	4.4342476456362126E-5	0.0	0.0	3.6966105497846287	0.0
37	4.4342476456362126E-5	0.0	0.0	3.8356685559517802	0.0
38	0.0031039733519453486	0.0	0.0	3.984792304274526	0.0
39	0.0031039733519453486	0.0	0.0	4.201405301763855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	90	0.0	42.500004	1
CGTTTTT	7890	0.0	40.38023	1
GCGATCG	165	0.0	39.545456	9
GGGCGAT	4035	0.0	39.479553	7
TATAGCG	115	0.0	39.130436	1
ACGGGAT	930	0.0	38.70968	5
TCGATAC	35	6.2509644E-6	38.571426	25
CACGACC	960	0.0	38.20312	27
AGGGCGA	2260	0.0	38.13053	6
TAAGGGA	3130	0.0	37.739616	4
GGTACGC	30	1.1402703E-4	37.499996	8
GCGAATA	30	1.1402703E-4	37.499996	12
GGCGATA	985	0.0	37.46193	8
AGGGATC	2295	0.0	37.352943	6
CGTAAGG	355	0.0	36.760563	2
GACCGAT	1215	0.0	36.48148	9
TAGGGAC	2500	0.0	36.449997	5
AAGGGAT	3345	0.0	36.390133	5
GGCGATT	1115	0.0	36.322872	8
AACGGGA	845	0.0	36.213017	4
>>END_MODULE