FastQCFastQC Report
Thu 26 May 2016
SRR1547963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547963_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2680559
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231600.86399888978381No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTC207.034971E-445.024
CGTTTTT145450.042.4011731
ACGCTAG950.040.2631571
GCGAACG900.040.01
CGCGTAA451.9299478E-840.031
CGAACGG1300.039.807692
CGGGTAT2050.039.5121966
TACGGGA12500.039.4200024
GGGCGAT45000.039.057
ACGGGAT15750.039.05
AGGGCGA23550.038.121026
TTAGGGA41500.038.0060234
TAGGGAT49850.037.7783365
CGGTTTT13250.037.6981121
TAGGGAC33750.037.6666685
AGGGATT60450.037.6302726
ACGGGAC9050.037.541445
CGGGATT14300.037.447556
CACGGGA9800.037.423474
GACCGAT13450.037.3048329
Produced by FastQC (version 0.11.5)