##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547963_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2680559
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.79280888799687	31.0	31.0	31.0	30.0	33.0
2	31.10712205924212	31.0	31.0	33.0	30.0	34.0
3	31.124297208156957	31.0	31.0	34.0	28.0	34.0
4	35.09275602588863	35.0	35.0	37.0	32.0	37.0
5	34.965283733728675	35.0	35.0	37.0	32.0	37.0
6	35.022092033788475	36.0	35.0	37.0	32.0	37.0
7	35.495827176346424	35.0	35.0	37.0	35.0	37.0
8	35.59900267071159	37.0	35.0	37.0	35.0	37.0
9	37.39076588129566	39.0	37.0	39.0	35.0	39.0
10	36.478060359798086	38.0	35.0	39.0	32.0	39.0
11	36.09207557080445	38.0	35.0	39.0	31.0	39.0
12	35.805933016210425	37.0	35.0	39.0	30.0	39.0
13	35.698968386817825	37.0	35.0	39.0	30.0	39.0
14	36.70734760921136	39.0	35.0	40.0	31.0	41.0
15	36.8382094928707	39.0	35.0	40.0	31.0	41.0
16	36.905917012085915	39.0	35.0	40.0	31.0	41.0
17	36.832539406892366	38.0	35.0	40.0	31.0	41.0
18	36.70156970990006	38.0	35.0	40.0	31.0	41.0
19	36.61458225690985	38.0	35.0	40.0	30.0	41.0
20	36.44837028395943	38.0	35.0	40.0	30.0	41.0
21	36.33896996857745	38.0	35.0	40.0	30.0	41.0
22	36.225249658746556	38.0	34.0	40.0	30.0	41.0
23	36.12238156295012	38.0	34.0	40.0	30.0	41.0
24	36.003430627716085	38.0	34.0	40.0	30.0	41.0
25	35.83295126128542	38.0	34.0	40.0	29.0	41.0
26	35.604341109447695	38.0	34.0	40.0	29.0	41.0
27	35.67774259025823	38.0	34.0	40.0	29.0	41.0
28	35.663817882762515	38.0	34.0	40.0	29.0	41.0
29	35.65029682241652	38.0	34.0	40.0	29.0	41.0
30	35.54043988585963	38.0	34.0	40.0	28.0	41.0
31	35.427608196648535	38.0	34.0	40.0	27.0	41.0
32	35.24816278992554	38.0	34.0	40.0	27.0	41.0
33	35.03542880421584	38.0	33.0	40.0	25.0	41.0
34	34.768311012740256	38.0	33.0	40.0	24.0	41.0
35	34.5296294541549	38.0	33.0	40.0	23.0	41.0
36	34.4204555840778	38.0	33.0	40.0	23.0	41.0
37	34.36155107945768	38.0	33.0	40.0	23.0	41.0
38	34.25784957540573	37.0	33.0	40.0	23.0	41.0
39	34.20664421115148	37.0	33.0	40.0	22.0	41.0
40	34.03172994886514	37.0	33.0	40.0	21.0	41.0
41	33.93799203822785	37.0	33.0	40.0	21.0	41.0
42	33.89380871676393	37.0	33.0	40.0	21.0	41.0
43	33.829553835599214	37.0	33.0	40.0	21.0	41.0
44	33.6795455723974	37.0	32.0	40.0	20.0	41.0
45	33.56447106741542	37.0	32.0	40.0	20.0	41.0
46	33.495633933071424	36.0	32.0	40.0	20.0	41.0
47	33.437749364964546	36.0	32.0	40.0	20.0	41.0
48	33.332685831574686	36.0	32.0	40.0	20.0	41.0
49	33.18931872046092	36.0	31.0	40.0	19.0	41.0
50	33.078880561852955	36.0	31.0	40.0	18.0	41.0
51	32.764761753052255	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	14.0
14	39.0
15	121.0
16	384.0
17	942.0
18	1899.0
19	3741.0
20	6299.0
21	9904.0
22	14942.0
23	21361.0
24	31123.0
25	44398.0
26	57890.0
27	64409.0
28	69400.0
29	76946.0
30	88530.0
31	104388.0
32	124243.0
33	152844.0
34	206623.0
35	237735.0
36	237280.0
37	284654.0
38	383802.0
39	456611.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.04979222617372	25.027242452040788	28.174160688125127	15.748804633660368
2	33.13461856276993	26.16786274803129	27.8187124402037	12.878806248995078
3	28.233998953203415	25.892397817022495	32.50433957991598	13.369263649858107
4	24.802326678875563	28.018969177697638	32.710042942535495	14.468661200891306
5	22.93890938419934	31.97288326800492	31.41576066783085	13.672446679964889
6	20.91030266448155	41.25404439894813	27.70463175777888	10.131021178791439
7	87.6967826486938	2.866678181677777	8.204333499094778	1.2322056705336462
8	88.30292487499808	2.3041089563781285	7.859927724030697	1.5330384445930867
9	83.15187242660951	5.465352562655775	9.30332068796098	2.07945432277372
10	35.28775154734517	39.26031846342498	15.606782018228287	9.845147971001571
11	26.928301149125982	26.063742674568996	30.663790649636884	16.34416552666813
12	26.502606359345197	23.986041717417898	31.38807987438441	18.123272048852495
13	22.81005566376267	27.202908050149244	32.247340946422	17.739695339666092
14	18.871772641452772	29.97885888726941	32.633902107732006	18.515466363545812
15	18.422911042062495	28.285331529729437	36.59684416571319	16.694913262494875
16	22.343175434676123	27.251144257597016	34.21924307579128	16.186437231935578
17	21.68465607360256	26.93143482385577	30.973352946157874	20.410556156383798
18	21.824291127335755	26.964711465033975	32.59491770186741	18.616079705762864
19	23.752993312215846	28.41146193760331	29.4625113642341	18.373033385946737
20	25.079470364203882	28.89595043421913	29.115680721819594	16.908898479757394
21	23.449847587760615	27.732984052953135	31.44691088687098	17.37025747241527
22	22.70858429156008	25.425405670981316	31.464034180930174	20.401975856528434
23	20.0439535186504	29.018835250408593	31.400092294181924	19.537118936759086
24	20.037648863539285	28.066048909947515	33.61586146770132	18.280440758811874
25	20.789917326945613	29.419609864957273	30.823981117371414	18.966491690725704
26	20.15292332681355	31.291905904701224	29.240878488404842	19.314292280080387
27	19.30086224552416	30.34949053536967	31.060648170773337	19.288999048332826
28	18.37885306758777	30.07682352822676	32.57148975269711	18.972833651488365
29	19.417815463117954	28.178935811522894	32.30509009501377	20.098158630345388
30	20.7234386558923	28.13905607002122	31.701410041711448	19.436095232375038
31	21.866670347490953	29.025512962035155	29.76685086953878	19.34096582093511
32	22.198951785802887	28.973956551599873	29.828181360678872	18.998910301918368
33	21.466082261199997	29.091208214406024	29.48052999393037	19.962179530463608
34	19.697346710145162	29.826838357223252	30.706692148913717	19.769122783717876
35	19.74782125668564	29.69656702202787	30.554298562352106	20.001313158934387
36	21.851897309479103	30.42943654663076	28.573890744430546	19.14477539945959
37	20.12863734765771	31.484664206234598	28.91997527381416	19.46672317229354
38	20.63550923520057	31.339918278239725	28.374268203012882	19.650304283546827
39	20.646141345890914	29.93058537417009	28.484842154192464	20.938431125746533
40	22.06159237681394	29.079307711563146	28.741318508564817	20.117781403058093
41	19.29269230783579	29.322876310500906	29.07330150166439	22.311129879998912
42	20.59835280626168	29.70059603239474	28.299544982968104	21.40150617837548
43	20.6061496874346	29.215958313172735	28.920273719026518	21.25761828036615
44	20.471438979705354	30.26018080557078	28.56434049763501	20.70403971708886
45	19.634225547730903	30.91239551153323	27.817891715869713	21.635487224866157
46	20.747053133320325	30.64801035903332	27.78901714157383	20.815919366072524
47	20.14564872476226	29.992885812250353	29.035995850119324	20.825469612868062
48	20.559069955184718	29.271207983110987	29.630871769657002	20.538850292047293
49	20.692810715973796	28.749227306692372	29.359249320757346	21.198712656576483
50	19.37480204688649	30.212914545063175	29.34078302324254	21.071500384807795
51	18.97354245886772	30.390862502933157	28.865658245164532	21.769936793034585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1359.0
1	1921.0
2	2483.0
3	20687.0
4	38891.0
5	29994.5
6	21098.0
7	19970.0
8	18842.0
9	20295.0
10	21748.0
11	22886.5
12	24025.0
13	24372.5
14	24720.0
15	23726.0
16	22732.0
17	21788.5
18	20845.0
19	20410.0
20	19975.0
21	20695.0
22	21415.0
23	22661.0
24	23907.0
25	26562.5
26	34301.0
27	39384.0
28	45088.0
29	50792.0
30	57780.0
31	64768.0
32	73482.0
33	82196.0
34	91733.0
35	101270.0
36	109548.5
37	117827.0
38	125215.5
39	132604.0
40	144113.0
41	155622.0
42	165988.5
43	176355.0
44	181387.5
45	186420.0
46	191911.5
47	197403.0
48	197362.5
49	197322.0
50	190098.0
51	182874.0
52	169220.5
53	155567.0
54	141090.0
55	126613.0
56	116579.0
57	106545.0
58	97954.5
59	89364.0
60	81345.5
61	73327.0
62	63342.5
63	53358.0
64	44973.5
65	36589.0
66	29794.0
67	22999.0
68	18556.5
69	14114.0
70	11918.0
71	9722.0
72	8155.0
73	6588.0
74	5442.5
75	3432.0
76	2567.0
77	2018.0
78	1469.0
79	1110.5
80	752.0
81	538.5
82	325.0
83	236.0
84	147.0
85	110.0
86	73.0
87	50.0
88	27.0
89	19.5
90	12.0
91	9.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2680559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.23267604788521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40705556633469	19.027218048302025
2	7.138755857937783	3.6025982789657385
3	3.002182939921499	2.2725932867858045
4	1.8766258588604319	1.8940916943883854
5	1.2390038115629796	1.5631690899631834
6	0.9604863052576432	1.454138387339778
7	0.7178288806278764	1.267892052188949
8	0.6045014315117437	1.220255103345496
9	0.5184689680679655	1.1774123560926284
>10	7.3374029681876864	44.88920936434994
>50	1.1518771739185287	18.53062845193032
>100	0.0436730340306149	1.715399734557056
>500	0.0013739167160417868	0.25361272021881304
>1k	6.106296515741275E-4	0.23966864295283333
>5k	0.0	0.0
>10k+	1.5265741289353186E-4	0.8921127886190325
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23160	0.86399888978381	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.730565154506952E-5	0.0	0.0	0.03398544855755833	0.0
2	3.730565154506952E-5	0.0	0.0	0.1056122995240918	0.0
3	3.730565154506952E-5	0.0	0.0	0.18399147342028285	0.0
4	3.730565154506952E-5	0.0	0.0	0.3007208571048054	0.0
5	3.730565154506952E-5	0.0	0.0	0.5653671491655285	0.0
6	3.730565154506952E-5	0.0	0.0	1.0710452558589458	0.0
7	3.730565154506952E-5	0.0	0.0	1.3733702559801892	0.0
8	3.730565154506952E-5	0.0	0.0	1.8972535206276004	0.0
9	3.730565154506952E-5	0.0	0.0	2.1764490167909005	0.0
10	3.730565154506952E-5	0.0	0.0	2.535068245093654	0.0
11	3.730565154506952E-5	0.0	0.0	2.8508232797711224	0.0
12	3.730565154506952E-5	0.0	0.0	3.0836851567154464	0.0
13	3.730565154506952E-5	0.0	0.0	3.229438337302033	0.0
14	3.730565154506952E-5	0.0	0.0	3.3241946922265093	0.0
15	3.730565154506952E-5	0.0	0.0	3.403245367850512	0.0
16	3.730565154506952E-5	0.0	0.0	3.52848044008731	0.0
17	3.730565154506952E-5	0.0	0.0	3.6768823219335967	0.0
18	3.730565154506952E-5	0.0	0.0	3.855352558925209	0.0
19	3.730565154506952E-5	0.0	0.0	3.977565873386857	0.0
20	3.730565154506952E-5	0.0	0.0	4.102502500411295	0.0
21	3.730565154506952E-5	0.0	0.0	4.256798675201702	0.0
22	3.730565154506952E-5	0.0	0.0	4.427098974504944	0.0
23	3.730565154506952E-5	0.0	0.0	4.626796127225702	0.0
24	3.730565154506952E-5	0.0	0.0	4.778480906407954	0.0
25	3.730565154506952E-5	0.0	0.0	4.921883830947202	0.0
26	3.730565154506952E-5	0.0	0.0	5.05521422956928	0.0
27	3.730565154506952E-5	0.0	0.0	5.198803682366253	0.0
28	3.730565154506952E-5	0.0	0.0	5.348511262016617	0.0
29	3.730565154506952E-5	0.0	0.0	5.5160136374539785	0.0
30	3.730565154506952E-5	0.0	0.0	5.717053793630359	0.0
31	3.730565154506952E-5	0.0	0.0	5.889107458556219	0.0
32	3.730565154506952E-5	0.0	0.0	6.069219144215815	0.0
33	3.730565154506952E-5	0.0	0.0	6.242765035203478	0.0
34	3.730565154506952E-5	0.0	0.0	6.414259115356162	0.0
35	3.730565154506952E-5	0.0	0.0	6.603734519553571	0.0
36	3.730565154506952E-5	0.0	0.0	6.783808899561621	0.0
37	3.730565154506952E-5	0.0	0.0	6.97652989544345	0.0
38	3.730565154506952E-5	0.0	0.0	7.187456049279273	0.0
39	3.730565154506952E-5	0.0	0.0	7.485938567291375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGTC	20	7.034971E-4	45.0	24
CGTTTTT	14545	0.0	42.401173	1
ACGCTAG	95	0.0	40.263157	1
GCGAACG	90	0.0	40.0	1
CGCGTAA	45	1.9299478E-8	40.0	31
CGAACGG	130	0.0	39.80769	2
CGGGTAT	205	0.0	39.512196	6
TACGGGA	1250	0.0	39.420002	4
GGGCGAT	4500	0.0	39.05	7
ACGGGAT	1575	0.0	39.0	5
AGGGCGA	2355	0.0	38.12102	6
TTAGGGA	4150	0.0	38.006023	4
TAGGGAT	4985	0.0	37.778336	5
CGGTTTT	1325	0.0	37.698112	1
TAGGGAC	3375	0.0	37.666668	5
AGGGATT	6045	0.0	37.630272	6
ACGGGAC	905	0.0	37.54144	5
CGGGATT	1430	0.0	37.44755	6
CACGGGA	980	0.0	37.42347	4
GACCGAT	1345	0.0	37.304832	9
>>END_MODULE