FastQCFastQC Report
Thu 26 May 2016
SRR1547962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547962_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4134477
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235880.5705195602732824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT186550.041.52641
CACGACC14650.038.7030727
GGGCGAT68000.038.613977
GCGATAT4450.037.921359
ACGGGTA9950.037.537695
AACACGT14750.037.37288341
ACGGGAT13400.037.2761155
ACGGGTC7900.037.0253145
GCGAGAC15200.037.0065821
AGGGCGA34850.036.9942636
TAGGGAT48800.036.8852465
GGCGATA15250.036.8852468
GTAGGGA46350.036.601944
GGCACCG16600.036.5963868
TAGGGTC28300.036.572445
TGGGCGA20200.036.534656
CGTTAGG6350.036.4960632
ACACGAC15600.036.49038326
ACGACCA15400.036.37986828
AGGGTAC27650.036.3743176
Produced by FastQC (version 0.11.5)