##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547962_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4134477
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.872859130671184	31.0	31.0	31.0	30.0	33.0
2	31.1997144983513	31.0	31.0	33.0	30.0	34.0
3	31.284205233213292	31.0	31.0	34.0	30.0	34.0
4	35.24012275313177	37.0	35.0	37.0	33.0	37.0
5	35.13109856458266	37.0	35.0	37.0	32.0	37.0
6	35.18108553996068	37.0	35.0	37.0	32.0	37.0
7	35.557235897067514	35.0	35.0	37.0	35.0	37.0
8	35.64561974827772	37.0	35.0	37.0	35.0	37.0
9	37.46343660878994	39.0	37.0	39.0	35.0	39.0
10	36.44191345120556	38.0	35.0	39.0	32.0	39.0
11	36.19720341895722	38.0	35.0	39.0	31.0	39.0
12	35.88288869426532	37.0	35.0	39.0	31.0	39.0
13	35.75729650932875	37.0	35.0	39.0	30.0	39.0
14	36.740220588964455	39.0	35.0	40.0	31.0	41.0
15	36.87218625233615	39.0	35.0	40.0	31.0	41.0
16	36.9516357207937	39.0	35.0	41.0	31.0	41.0
17	36.86950683242403	38.0	35.0	40.0	31.0	41.0
18	36.7562535720963	38.0	35.0	40.0	31.0	41.0
19	36.68346056828953	38.0	35.0	40.0	31.0	41.0
20	36.538634995429895	38.0	35.0	40.0	30.0	41.0
21	36.44965348700694	38.0	35.0	40.0	30.0	41.0
22	36.336280985478936	38.0	35.0	40.0	30.0	41.0
23	36.24293060524947	38.0	34.0	40.0	30.0	41.0
24	36.12508498656541	38.0	34.0	40.0	30.0	41.0
25	35.96022278029361	38.0	34.0	40.0	30.0	41.0
26	35.753956304509614	38.0	34.0	40.0	29.0	41.0
27	35.86436760925263	38.0	34.0	40.0	29.0	41.0
28	35.8872590172832	38.0	34.0	40.0	30.0	41.0
29	35.86756994899234	38.0	34.0	40.0	29.0	41.0
30	35.75714993698114	38.0	34.0	40.0	29.0	41.0
31	35.65329036780226	38.0	34.0	40.0	29.0	41.0
32	35.47707243261965	38.0	34.0	40.0	27.0	41.0
33	35.279589897343726	38.0	34.0	40.0	27.0	41.0
34	35.023557030308794	38.0	34.0	40.0	25.0	41.0
35	34.79794445585258	38.0	33.0	40.0	24.0	41.0
36	34.71253365298682	38.0	33.0	40.0	24.0	41.0
37	34.65888720628994	38.0	33.0	40.0	24.0	41.0
38	34.55898968599898	38.0	33.0	40.0	23.0	41.0
39	34.50787173323252	38.0	33.0	40.0	23.0	41.0
40	34.344950522157944	37.0	33.0	40.0	23.0	41.0
41	34.265774365173634	37.0	33.0	40.0	23.0	41.0
42	34.23004046219147	37.0	33.0	40.0	23.0	41.0
43	34.18715426400969	37.0	33.0	40.0	23.0	41.0
44	34.04833162695064	37.0	33.0	40.0	22.0	41.0
45	33.921855412425806	37.0	33.0	40.0	22.0	41.0
46	33.858960879453434	37.0	33.0	40.0	22.0	41.0
47	33.80370262067004	37.0	33.0	40.0	22.0	41.0
48	33.71872669747589	36.0	32.0	40.0	22.0	41.0
49	33.595103080752416	36.0	32.0	40.0	21.0	41.0
50	33.51161126304488	36.0	32.0	40.0	20.0	41.0
51	33.19504183963292	36.0	31.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	14.0
14	70.0
15	173.0
16	420.0
17	1178.0
18	2544.0
19	4691.0
20	8253.0
21	12808.0
22	19544.0
23	28678.0
24	42716.0
25	61768.0
26	80817.0
27	91112.0
28	97990.0
29	109609.0
30	128602.0
31	155004.0
32	188954.0
33	236137.0
34	331487.0
35	373283.0
36	365449.0
37	453272.0
38	611776.0
39	728052.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.82670988374104	25.59569203069699	28.571594424155705	14.006003661406266
2	32.060258165664	26.84216165672224	27.75064415644349	13.346936021170272
3	29.432864180886725	25.613517743598525	31.394684261153223	13.558933814361525
4	25.25446386568362	28.248530588028427	31.555139864123078	14.94186568216488
5	23.50872915727914	32.09532910692211	30.31527324979677	14.08066848600198
6	21.793397326917045	41.23542107018615	26.900959903755663	10.070221699141149
7	89.50912050060987	2.581680826861535	6.919980447345578	0.9892182251830158
8	89.83162803904823	2.1605634763477943	6.812494059103486	1.1953144255004926
9	84.76288536615392	4.275026805083206	9.025833255330722	1.9362545734321415
10	34.933559915800714	28.264445539302795	25.412573343617584	11.389421201278903
11	30.652800825836014	26.0920546903514	26.068593439992533	17.186551043820053
12	28.813849974253092	24.053513902725786	28.72256877955785	18.410067343463272
13	24.41143099840681	26.630139676674947	30.122890996854018	18.835538328064228
14	20.692919563949687	28.472307380111197	31.634037388525805	19.200735667413316
15	21.194047034243994	26.593448216062153	34.76084157681854	17.45166317287531
16	24.657991808879334	25.418354002211164	32.57471259363639	17.348941595273114
17	23.955750630611806	25.10310252058483	30.065350466334678	20.87579638246869
18	24.318214855228366	25.19484326554483	31.955529079010475	18.531412800216327
19	25.42599704871983	27.224313982155422	28.745304424235517	18.604384544889232
20	26.719800352015504	27.304227354511827	28.893835907177618	17.082136386295048
21	25.429818571974156	26.677497540801415	30.5512160304677	17.341467856756733
22	24.79290609187087	23.66654839294063	31.116293548131964	20.424251967056534
23	22.10971303020914	26.782468496015337	31.287270433479254	19.82054804029627
24	21.84772100558305	26.40795921709082	33.188671747357645	18.555648029968484
25	22.350589929512246	27.734511523464754	30.471157536975053	19.443741010047948
26	21.512031630602856	28.84014108676865	29.277560378253405	20.370266904375086
27	20.350240187573906	28.68007247349544	31.086809770619112	19.882877568311542
28	18.99014071187238	28.752439546767345	32.36868895388703	19.888730787473243
29	20.55786015982191	27.10028378438192	32.16247181928936	20.179384236506817
30	21.602635593328976	27.17961667219336	32.173718707347994	19.04402902712967
31	23.85762455565722	27.262359906706457	29.790200792022787	19.08981474561353
32	24.205891095778256	26.814419332844274	29.961564667066714	19.018124904310753
33	23.902031623346797	27.567694777356362	28.762452905167933	19.767820694128908
34	20.925185942502523	28.031574489348955	31.075248453431957	19.967991114716565
35	21.42989306749076	28.15543537913018	30.727804266416285	19.686867286962777
36	23.583442355586932	29.71275447898247	28.048118298880365	18.65568486655023
37	21.734937695868183	29.694686897520533	28.51574697355917	20.054628433052113
38	22.35477425560718	29.740859605701036	27.79746990973707	20.106896228954714
39	21.777554936210795	28.72960715466551	28.16992330589818	21.32291460322551
40	23.538575737632595	27.527254354057355	28.452570905582498	20.481599002727553
41	20.511058593384362	27.426637032930646	29.11400885771042	22.94829551597457
42	21.683975990191744	27.466013234563892	28.69973638745602	22.150274387788347
43	21.60756971196115	27.186074562756062	29.242513623851334	21.96384210143145
44	21.379898836055926	27.906794499038213	28.78015768378927	21.933148981116595
45	21.090841719520995	29.057726043705166	27.727231279796694	22.12420095697715
46	21.798017016420697	28.844301226007545	28.254383807190127	21.103297950381634
47	21.0937441422458	28.404245567214424	29.54726800995628	20.954742280583496
48	21.715346342475723	28.027245042117784	29.89466382325987	20.362744792146625
49	22.166818197319756	27.310491750226205	29.69693627513226	20.825753777321776
50	21.07642635332111	28.343173755713234	29.28225262832518	21.298147262640473
51	20.410151029985172	29.098964633253495	28.630561979181408	21.860322357579932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1148.0
1	2592.5
2	4037.0
3	25753.0
4	47469.0
5	37632.5
6	27796.0
7	25863.0
8	23930.0
9	24139.5
10	24349.0
11	24550.5
12	24752.0
13	24166.5
14	23581.0
15	23055.5
16	22530.0
17	21768.0
18	21006.0
19	20983.5
20	20961.0
21	21350.0
22	21739.0
23	24493.5
24	27248.0
25	31822.0
26	43891.0
27	51386.0
28	60157.5
29	68929.0
30	83370.0
31	97811.0
32	110926.0
33	124041.0
34	140353.5
35	156666.0
36	165157.5
37	173649.0
38	189700.0
39	205751.0
40	224115.5
41	242480.0
42	257518.5
43	272557.0
44	276943.5
45	281330.0
46	286732.0
47	292134.0
48	289144.0
49	286154.0
50	281700.0
51	277246.0
52	262279.5
53	247313.0
54	230260.0
55	213207.0
56	202066.0
57	190925.0
58	177696.0
59	164467.0
60	150740.0
61	137013.0
62	119891.0
63	102769.0
64	89054.0
65	75339.0
66	63240.0
67	51141.0
68	41963.5
69	32786.0
70	27482.5
71	22179.0
72	19100.0
73	16021.0
74	13292.0
75	8689.5
76	6816.0
77	5031.0
78	3246.0
79	2483.5
80	1721.0
81	1352.5
82	984.0
83	733.5
84	483.0
85	350.0
86	217.0
87	161.0
88	105.0
89	91.5
90	78.0
91	51.0
92	24.0
93	14.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4134477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.768925251464072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75028355025356	16.147898273353526
2	6.770186082305646	2.812189773638167
3	2.260863341059383	1.4086710520271293
4	1.1849312984328777	0.984389982610908
5	0.8005598478144335	0.8313383819290711
6	0.6098272696348517	0.7599274187610394
7	0.4666469453883375	0.6784228869316092
8	0.4083978563304456	0.678558764078815
9	0.35962606512664064	0.6722142178584014
>10	7.03089542442594	38.64629276668264
>50	2.1841628214994055	30.4699432678585
>100	0.17024942061081685	4.7510961231139195
>500	0.0027682776323399447	0.3690624559263953
>1k	4.8143958822769215E-4	0.200354618275719
>5k	0.0	0.0
>10k+	1.2035989705692304E-4	0.5896400169541682
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23588	0.5705195602732824	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4186856040074718E-5	0.0	0.0	0.010666403513672951	0.0
2	2.4186856040074718E-5	0.0	0.0	0.04619689503654271	0.0
3	2.4186856040074718E-5	0.0	0.0	0.07207683099942266	0.0
4	2.4186856040074718E-5	0.0	0.0	0.12323203152418069	0.0
5	2.4186856040074718E-5	0.0	0.0	0.24007873305378166	0.0
6	2.4186856040074718E-5	0.0	0.0	0.44085382504244186	0.0
7	2.4186856040074718E-5	0.0	0.0	0.5470824967704501	0.0
8	2.4186856040074718E-5	0.0	0.0	0.7971262145127425	0.0
9	2.4186856040074718E-5	0.0	0.0	0.9003073423797012	0.0
10	2.4186856040074718E-5	0.0	0.0	1.077621184009489	0.0
11	2.4186856040074718E-5	0.0	0.0	1.2762194589545426	0.0
12	2.4186856040074718E-5	0.0	0.0	1.4020636709310512	0.0
13	2.4186856040074718E-5	0.0	0.0	1.4606684231161522	0.0
14	2.4186856040074718E-5	0.0	0.0	1.4891847263874003	0.0
15	2.4186856040074718E-5	0.0	0.0	1.533059683244096	0.0
16	2.4186856040074718E-5	0.0	0.0	1.6408121269026288	0.0
17	2.4186856040074718E-5	0.0	0.0	1.7676238131207405	0.0
18	2.4186856040074718E-5	0.0	0.0	1.9188400370832877	0.0
19	2.4186856040074718E-5	0.0	0.0	1.9983906065990935	0.0
20	2.4186856040074718E-5	0.0	0.0	2.0880996556517304	0.0
21	2.4186856040074718E-5	0.0	0.0	2.21092051062323	0.0
22	2.4186856040074718E-5	0.0	0.0	2.3544211275089935	0.0
23	2.4186856040074718E-5	0.0	0.0	2.514634861918448	0.0
24	2.4186856040074718E-5	0.0	0.0	2.6415916692728003	0.0
25	2.4186856040074718E-5	0.0	0.0	2.755076397812831	0.0
26	2.4186856040074718E-5	0.0	0.0	2.8701090851394264	0.0
27	2.4186856040074718E-5	0.0	0.0	3.0041768281695607	0.0
28	2.4186856040074718E-5	0.0	0.0	3.1342295530970423	0.0
29	2.4186856040074718E-5	0.0	0.0	3.283389894296183	0.0
30	2.4186856040074718E-5	0.0	0.0	3.453350931689788	0.0
31	2.4186856040074718E-5	0.0	0.0	3.6027531414493295	0.0
32	2.4186856040074718E-5	0.0	0.0	3.7500994684454647	0.0
33	2.4186856040074718E-5	0.0	0.0	3.9079912646750725	0.0
34	2.4186856040074718E-5	0.0	0.0	4.077226696387475	0.0
35	2.4186856040074718E-5	0.0	0.0	4.271326216109075	0.0
36	2.4186856040074718E-5	0.0	0.0	4.444745973916411	0.0
37	2.4186856040074718E-5	0.0	0.0	4.636015631481322	0.0
38	2.4186856040074718E-5	0.0	0.0	4.850069307435983	0.0
39	2.4186856040074718E-5	2.4186856040074718E-5	0.0	5.148027186993663	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18655	0.0	41.5264	1
CACGACC	1465	0.0	38.70307	27
GGGCGAT	6800	0.0	38.61397	7
GCGATAT	445	0.0	37.92135	9
ACGGGTA	995	0.0	37.53769	5
AACACGT	1475	0.0	37.372883	41
ACGGGAT	1340	0.0	37.276115	5
ACGGGTC	790	0.0	37.025314	5
GCGAGAC	1520	0.0	37.00658	21
AGGGCGA	3485	0.0	36.994263	6
TAGGGAT	4880	0.0	36.885246	5
GGCGATA	1525	0.0	36.885246	8
GTAGGGA	4635	0.0	36.60194	4
GGCACCG	1660	0.0	36.596386	8
TAGGGTC	2830	0.0	36.57244	5
TGGGCGA	2020	0.0	36.53465	6
CGTTAGG	635	0.0	36.496063	2
ACACGAC	1560	0.0	36.490383	26
ACGACCA	1540	0.0	36.379868	28
AGGGTAC	2765	0.0	36.374317	6
>>END_MODULE