FastQCFastQC Report
Thu 26 May 2016
SRR1547961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547961_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1576365
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62330.39540334884370054No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG207.034086E-445.02
GTAACGA207.034086E-445.018
CACGACG2250.043.00000426
TAGTACG1000.042.751
CGTAAGG1800.042.52
TCACGAC2300.041.08695625
GGTACGC501.0822987E-940.58
CGACGGT2450.040.40816528
TCTCACG2300.040.10869223
CAGTTCG451.9290383E-840.01
CGTTTTT41200.039.8665051
CGGTCTA2350.039.25531831
TAACGGG3500.039.2142873
TTAGGCG1150.039.1304321
GCGAACT356.2497656E-638.57142624
GCGATAC659.094947E-1238.076929
CGAGGGT1600.037.968754
TAGGGTA7950.037.924535
TGGGCGA6000.037.8750046
GTAGGGT8800.037.8409084
Produced by FastQC (version 0.11.5)