##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547961_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1576365
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.824549517402378	31.0	31.0	31.0	30.0	33.0
2	31.16201450806127	31.0	31.0	33.0	30.0	34.0
3	31.30975694081003	31.0	31.0	34.0	30.0	34.0
4	35.19958892769124	35.0	35.0	37.0	33.0	37.0
5	35.031665889562376	35.0	35.0	37.0	32.0	37.0
6	35.0668715684502	37.0	35.0	37.0	32.0	37.0
7	35.48723994760097	35.0	35.0	37.0	35.0	37.0
8	35.56892915029197	37.0	35.0	37.0	35.0	37.0
9	37.37351565151472	39.0	37.0	39.0	35.0	39.0
10	36.44240261614537	38.0	35.0	39.0	32.0	39.0
11	36.05705721707853	38.0	35.0	39.0	31.0	39.0
12	35.53080282802524	37.0	35.0	39.0	30.0	39.0
13	35.282116134270936	37.0	35.0	39.0	30.0	39.0
14	36.25897999511535	38.0	35.0	40.0	30.0	41.0
15	36.46605196131607	38.0	35.0	40.0	30.0	41.0
16	36.59204625832215	38.0	35.0	40.0	31.0	41.0
17	36.53490340117929	38.0	35.0	40.0	31.0	41.0
18	36.4541606797918	38.0	35.0	40.0	30.0	41.0
19	36.3913199037025	38.0	35.0	40.0	30.0	41.0
20	36.26895484231127	38.0	34.0	40.0	30.0	41.0
21	36.13316268757553	38.0	34.0	40.0	30.0	41.0
22	36.01804785059298	38.0	34.0	40.0	30.0	41.0
23	35.920069907667326	38.0	34.0	40.0	30.0	41.0
24	35.799328201273184	38.0	34.0	40.0	29.0	41.0
25	35.63590031496513	37.0	34.0	40.0	29.0	41.0
26	35.392434493280426	37.0	34.0	40.0	27.0	41.0
27	35.45245549095546	37.0	34.0	40.0	28.0	41.0
28	35.47879329977511	37.0	34.0	40.0	28.0	41.0
29	35.45439666574683	37.0	34.0	40.0	28.0	41.0
30	35.35614467461534	37.0	34.0	40.0	27.0	41.0
31	35.244238485376165	37.0	34.0	40.0	27.0	41.0
32	35.091812492665085	37.0	34.0	40.0	27.0	41.0
33	34.94251458259984	37.0	33.0	40.0	26.0	41.0
34	34.750439777589584	37.0	33.0	40.0	25.0	41.0
35	34.62281324439454	37.0	33.0	40.0	24.0	41.0
36	34.51543392551852	37.0	33.0	40.0	24.0	41.0
37	34.47366060525322	37.0	33.0	40.0	24.0	41.0
38	34.38510750999927	37.0	33.0	40.0	24.0	41.0
39	34.34074722542051	37.0	33.0	40.0	23.0	41.0
40	34.14643816628763	37.0	33.0	40.0	23.0	41.0
41	34.053096205510776	36.0	33.0	40.0	23.0	41.0
42	34.016086375934506	36.0	33.0	40.0	23.0	41.0
43	33.96350210769714	36.0	33.0	40.0	23.0	41.0
44	33.80366983534905	36.0	33.0	40.0	23.0	41.0
45	33.673279982745115	36.0	32.0	40.0	23.0	41.0
46	33.60238079378824	36.0	32.0	40.0	22.0	41.0
47	33.550261519381614	35.0	32.0	40.0	22.0	41.0
48	33.43572269112801	35.0	32.0	40.0	22.0	41.0
49	33.28293320392168	35.0	32.0	39.0	21.0	41.0
50	33.16795665978374	35.0	32.0	39.0	20.0	41.0
51	32.835298931402306	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	12.0
14	37.0
15	102.0
16	250.0
17	576.0
18	1232.0
19	2214.0
20	3766.0
21	5881.0
22	8682.0
23	12494.0
24	17530.0
25	23612.0
26	29406.0
27	34410.0
28	38893.0
29	45508.0
30	53997.0
31	64697.0
32	77458.0
33	97125.0
34	139761.0
35	158729.0
36	136489.0
37	162347.0
38	212938.0
39	248194.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.14841423147558	25.466881084012904	28.342230384460454	13.042474300051065
2	31.33252768235783	27.361873677733268	28.016480954601253	13.289117685307655
3	29.463100233765655	25.96372033126846	30.08535459744412	14.487824837521767
4	25.541356221433485	29.23904045065705	30.434322000298152	14.785281327611308
5	23.262632702451526	32.77787821982853	29.396618169015426	14.56287090870452
6	21.322980401112687	42.133960091730025	26.568212311235023	9.974847195922264
7	89.78282314057975	3.429599109343331	5.416258290434005	1.3713194596429126
8	90.1840627012145	2.838048294652571	5.147729110960976	1.8301598931719496
9	85.4923827920564	5.313299901989704	6.761885730779356	2.4324315751745313
10	42.44461149543412	30.081040875685517	14.578095809029001	12.896251819851368
11	34.31343629172178	26.369590799085234	22.39081684762095	16.926156061572033
12	31.54034757178699	23.726865288178818	26.827479676343994	17.9053074636902
13	24.342649069219373	30.234114561031234	27.68971653138708	17.73351983836231
14	19.883339201263666	33.31385814833494	28.756474547455696	18.0463281029457
15	18.759804994401676	28.05771505964672	35.68177420838448	17.500705737567124
16	21.71736875660142	25.24275786381961	35.16748976284046	17.87238361673851
17	21.74635950430262	25.857209466081777	27.476821675183093	24.919609354432506
18	23.415960136135983	26.810605411817694	30.42797829182962	19.3454561602167
19	25.929971802215857	28.667979814319654	25.956551940699015	19.445496442765474
20	27.29938814931821	28.294018200099597	25.8532129297466	18.553380720835595
21	25.02770614673632	28.186872964066062	28.397801270644806	18.387619618552808
22	24.030919235075633	25.6589685764401	27.72631972925052	22.58379245923374
23	21.52604250919045	29.672379176142584	27.034728632010985	21.766849682655984
24	21.75790505371535	28.1728533683506	30.956409207258474	19.112832370675573
25	21.803896940112217	28.561278637878917	29.237073901031803	20.39775052097706
26	20.987651971465997	32.19996637834512	26.344025653957047	20.46835599623184
27	20.541816140297456	31.279494279560886	28.353205000111014	19.82548458003064
28	19.54382392402775	29.65988207045957	31.2582428561913	19.53805114932138
29	20.07929635585667	28.47475045436812	30.94549802869259	20.500455161082616
30	21.536636502332897	29.253123483457195	29.29004386674406	19.920196147465845
31	25.248720949780036	28.566861101331227	26.491453438765767	19.692964510122973
32	25.967336245095517	28.825050036000544	26.513402670066892	18.69421104883704
33	25.428691959032328	28.849980810281885	25.616085107192816	20.10524212349297
34	21.04220786429539	29.247794768343628	28.305246564088897	21.404750803272083
35	21.790448278158927	29.311041541774905	28.010644742810197	20.887865437255964
36	25.625981292403726	29.007812276979	26.118697129154732	19.247509301462543
37	21.909329374859247	31.958842019456153	26.352209037881458	19.77961956780314
38	21.868412455237205	32.00902075344225	25.170439587278327	20.952127204042213
39	21.140598782642346	30.513110859477344	26.717670082753674	21.628620275126636
40	23.540614007542672	28.469802361762664	26.937606455357738	21.05197717533693
41	20.16011520174579	28.14176919685479	28.01127911365705	23.68683648774237
42	22.213256447586694	29.04809482575419	26.28154012554199	22.457108601117127
43	21.885032971424764	28.397103462713268	26.91210474731423	22.805758818547737
44	20.92611799932122	29.743428710990155	28.01470471623006	21.31574857345856
45	20.58894989421866	31.831904413000796	25.390820019475186	22.188325673305357
46	22.298008392726302	30.457920595801095	26.581280350680203	20.662790660792393
47	21.993827571660116	29.663117361778525	26.998506056655657	21.3445490099057
48	22.327633511274357	28.574727299832208	28.117155607996878	20.980483580896557
49	21.597726414884878	27.71623323278555	29.121999029412603	21.564041322916964
50	20.88482045719107	30.19059672093709	27.69606023985562	21.22852258201622
51	20.080501660465693	31.17583808318505	26.541251550243754	22.2024087061055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1026.0
1	1456.5
2	1887.0
3	6572.0
4	11257.0
5	8855.0
6	6453.0
7	6352.5
8	6252.0
9	7180.0
10	8108.0
11	8727.0
12	9346.0
13	9805.5
14	10265.0
15	9889.5
16	9514.0
17	9505.0
18	9496.0
19	9613.0
20	9730.0
21	9621.5
22	9513.0
23	10672.0
24	11831.0
25	13610.0
26	16937.0
27	18485.0
28	20948.0
29	23411.0
30	27405.0
31	31399.0
32	35813.0
33	40227.0
34	43715.0
35	47203.0
36	51615.5
37	56028.0
38	62175.0
39	68322.0
40	81802.0
41	95282.0
42	103158.0
43	111034.0
44	114090.5
45	117147.0
46	118547.5
47	119948.0
48	122052.0
49	124156.0
50	117618.0
51	111080.0
52	102451.5
53	93823.0
54	86785.5
55	79748.0
56	74524.0
57	69300.0
58	65798.5
59	62297.0
60	57950.5
61	53604.0
62	47115.0
63	40626.0
64	35251.0
65	29876.0
66	25806.5
67	21737.0
68	18341.0
69	14945.0
70	12162.5
71	9380.0
72	8339.5
73	7299.0
74	5840.5
75	3451.5
76	2521.0
77	1958.5
78	1396.0
79	1112.5
80	829.0
81	600.0
82	371.0
83	300.0
84	229.0
85	169.5
86	110.0
87	84.0
88	58.0
89	44.5
90	31.0
91	21.0
92	11.0
93	6.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1576365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.322617947491306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.27759018172928	19.82193510022227
2	7.132074455160803	3.6120559320219847
3	2.755091192039399	2.0929836499953622
4	1.5368105811987847	1.5566426882143554
5	0.9614592751974281	1.2173332948948186
6	0.7686008156983292	1.167779088603538
7	0.612361615725448	1.0854619468506803
8	0.49199554169026305	0.9966892107273249
9	0.40946112584330163	0.9331764888671626
>10	5.288186648203315	31.47084104294504
>50	1.4862907366677052	26.346052421755832
>100	0.27803346712683824	8.821052976689705
>500	0.0010221818595406102	0.20040935303525892
>1k	7.666363946554577E-4	0.27424437783772276
>5k	2.5554546488515254E-4	0.4033424273389632
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6233	0.39540334884370054	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01719144994972611	0.0
2	0.0	0.0	0.0	0.07758355457016618	0.0
3	0.0	0.0	0.0	0.12611292435444837	0.0
4	0.0	0.0	0.0	0.21777951172475918	0.0
5	0.0	0.0	0.0	0.42027068604035234	0.0
6	0.0	0.0	0.0	0.7784999032584459	0.0
7	0.0	0.0	0.0	0.9537131311593444	0.0
8	0.0	0.0	0.0	1.3602179698229788	0.0
9	0.0	0.0	0.0	1.5450736345960485	0.0
10	0.0	0.0	0.0	1.8576281508407	0.0
11	0.0	0.0	0.0	2.24161282444104	0.0
12	0.0	0.0	0.0	2.5270162684403674	0.0
13	0.0	0.0	0.0	2.687829278117695	0.0
14	0.0	0.0	0.0	2.7709952961401707	0.0
15	0.0	0.0	0.0	2.8607587709699214	0.0
16	0.0	0.0	0.0	3.0293111049788597	0.0
17	0.0	0.0	0.0	3.2200029815429803	0.0
18	0.0	0.0	0.0	3.4440627646515876	0.0
19	0.0	0.0	0.0	3.584512470144922	0.0
20	0.0	0.0	0.0	3.7228687518436403	0.0
21	0.0	0.0	0.0	3.9057578669914643	0.0
22	0.0	0.0	0.0	4.104760001649364	0.0
23	0.0	0.0	0.0	4.317845169107408	0.0
24	0.0	0.0	0.0	4.488173741487536	0.0
25	0.0	0.0	0.0	4.641247426833252	0.0
26	0.0	0.0	0.0	4.78245837734281	0.0
27	0.0	0.0	0.0	4.934643943502932	0.0
28	0.0	0.0	0.0	5.1005319199550865	0.0
29	0.0	0.0	0.0	5.28507039930473	0.0
30	0.0	0.0	0.0	5.486356269011301	0.0
31	0.0	0.0	0.0	5.673241920494302	0.0
32	0.0	0.0	0.0	5.849279830496109	0.0
33	0.0	0.0	0.0	6.02855303181687	0.0
34	0.0	0.0	0.0	6.207572484798889	0.0
35	0.0	0.0	0.0	6.414250506703714	0.0
36	0.0	0.0	0.0	6.608114237502101	0.0
37	0.0	0.0	0.0	6.81162040517266	0.0
38	0.0	0.0	0.0	7.027433367272174	0.0
39	0.0	0.0	0.0	7.332946367116753	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	20	7.034086E-4	45.0	2
GTAACGA	20	7.034086E-4	45.0	18
CACGACG	225	0.0	43.000004	26
TAGTACG	100	0.0	42.75	1
CGTAAGG	180	0.0	42.5	2
TCACGAC	230	0.0	41.086956	25
GGTACGC	50	1.0822987E-9	40.5	8
CGACGGT	245	0.0	40.408165	28
TCTCACG	230	0.0	40.108692	23
CAGTTCG	45	1.9290383E-8	40.0	1
CGTTTTT	4120	0.0	39.866505	1
CGGTCTA	235	0.0	39.255318	31
TAACGGG	350	0.0	39.214287	3
TTAGGCG	115	0.0	39.130432	1
GCGAACT	35	6.2497656E-6	38.571426	24
GCGATAC	65	9.094947E-12	38.07692	9
CGAGGGT	160	0.0	37.96875	4
TAGGGTA	795	0.0	37.92453	5
TGGGCGA	600	0.0	37.875004	6
GTAGGGT	880	0.0	37.840908	4
>>END_MODULE