FastQCFastQC Report
Thu 26 May 2016
SRR1547959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547959_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2213836
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99170.44795549444493626No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39380.17788128840618725No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG253.8919803E-545.0000041
TCCGCAT406.8193913E-945.036
CTATCGA207.034704E-445.027
CGTTGTA207.034704E-445.034
TCTATCG351.2126111E-745.026
TAACGCC600.044.99999612
CACTCGA302.1664182E-644.99999627
CGACGGT6750.043.33333628
TACGGGA8500.041.8235324
CGGTCTA7100.040.88028331
CGTTTTT59150.040.6635671
AGGGCGA18400.040.4755486
GGCGATA8300.040.3915638
TAGGGCG7200.040.31255
CGTTAAC451.9297659E-840.020
GACGATT451.9297659E-840.09
GATCGAC800.039.3759
CACGACG7450.038.95973226
GACGGTC7500.038.729
GGGCGAT36400.038.6950577
Produced by FastQC (version 0.11.5)