##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547959_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2213836
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45512314371977	31.0	31.0	33.0	30.0	34.0
2	31.87202620248293	31.0	31.0	34.0	30.0	34.0
3	31.983248985019667	33.0	31.0	34.0	30.0	34.0
4	35.748912295219704	37.0	35.0	37.0	35.0	37.0
5	35.653685729204874	37.0	35.0	37.0	33.0	37.0
6	35.70942020998845	37.0	35.0	37.0	33.0	37.0
7	35.985461886065636	37.0	35.0	37.0	35.0	37.0
8	35.99566860417845	37.0	35.0	37.0	35.0	37.0
9	37.754609194176986	39.0	38.0	39.0	35.0	39.0
10	37.263742210353435	39.0	37.0	39.0	34.0	39.0
11	37.069193472325864	39.0	37.0	39.0	33.0	39.0
12	36.492394648926116	39.0	35.0	39.0	33.0	39.0
13	36.26745748104196	39.0	35.0	39.0	32.0	39.0
14	37.33207383022049	39.0	35.0	41.0	32.0	41.0
15	37.51899418023738	39.0	35.0	41.0	33.0	41.0
16	37.612646555571416	39.0	35.0	41.0	33.0	41.0
17	37.53420397897586	39.0	35.0	41.0	33.0	41.0
18	37.44180372891217	39.0	36.0	41.0	33.0	41.0
19	37.39247803360321	39.0	35.0	41.0	32.0	41.0
20	37.26895985068451	39.0	35.0	41.0	32.0	41.0
21	37.15368211556773	39.0	35.0	41.0	32.0	41.0
22	37.06303989997453	39.0	35.0	41.0	32.0	41.0
23	36.94345561279155	39.0	35.0	41.0	32.0	41.0
24	36.90303301599577	38.0	35.0	41.0	32.0	41.0
25	36.945392070596014	39.0	35.0	41.0	32.0	41.0
26	36.86242928563814	39.0	35.0	41.0	32.0	41.0
27	36.78874135211461	38.0	35.0	41.0	31.0	41.0
28	36.74740043977964	38.0	35.0	41.0	31.0	41.0
29	36.76851718013439	38.0	35.0	41.0	31.0	41.0
30	36.71559139882087	38.0	35.0	41.0	31.0	41.0
31	36.59049992863066	38.0	35.0	41.0	31.0	41.0
32	36.44965751753969	38.0	35.0	41.0	31.0	41.0
33	36.318893992147565	38.0	35.0	41.0	30.0	41.0
34	36.19428403910678	38.0	35.0	41.0	30.0	41.0
35	36.114183254766836	38.0	35.0	41.0	30.0	41.0
36	36.03918718459723	38.0	35.0	41.0	30.0	41.0
37	35.94982148632509	38.0	35.0	41.0	30.0	41.0
38	35.86659129221857	38.0	35.0	40.0	29.0	41.0
39	35.817982000473386	38.0	35.0	40.0	29.0	41.0
40	35.67013907082548	38.0	35.0	40.0	29.0	41.0
41	35.60141085428189	38.0	35.0	40.0	28.0	41.0
42	35.58431970570539	38.0	35.0	40.0	28.0	41.0
43	35.51846929944224	38.0	35.0	40.0	28.0	41.0
44	35.35250985167826	38.0	34.0	40.0	27.0	41.0
45	35.229408592145035	38.0	34.0	40.0	27.0	41.0
46	35.18147956759218	38.0	34.0	40.0	27.0	41.0
47	35.13865841914216	37.0	34.0	40.0	27.0	41.0
48	35.033558493041035	37.0	34.0	40.0	27.0	41.0
49	34.9306095844498	37.0	34.0	40.0	26.0	41.0
50	34.82404026314506	37.0	34.0	40.0	26.0	41.0
51	34.50200918225198	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	7.0
13	9.0
14	12.0
15	49.0
16	139.0
17	327.0
18	671.0
19	1339.0
20	2385.0
21	4002.0
22	6141.0
23	9219.0
24	13928.0
25	20476.0
26	26592.0
27	31722.0
28	35016.0
29	40411.0
30	49006.0
31	60359.0
32	75853.0
33	101475.0
34	175854.0
35	258729.0
36	159131.0
37	209606.0
38	332540.0
39	598719.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.54253521941101	24.628292249290375	30.11835565055406	13.710816880744552
2	31.283482606661018	25.32161370580296	29.727721475303497	13.667182212232523
3	28.66016272208059	25.613866609812106	32.042978793370416	13.682991874736883
4	25.986297087950504	27.813848902990102	31.063863809243326	15.135990199816066
5	23.993963419151193	32.70901728944691	29.04998382897378	14.247035462428112
6	23.09805243026132	40.4631598727277	26.0603766493995	10.378411047611475
7	88.9832399509268	3.518146782327146	5.809779947566125	1.688833319179921
8	89.11942890078579	2.911507446802744	5.838869726574146	2.130193925837325
9	83.78434536252911	6.0292632335909255	7.425346773654418	2.761044630225545
10	36.265784818749	40.61809456527042	12.576857545003334	10.539263070977253
11	37.03887731521215	22.982370871193712	24.430129422414307	15.548622391179833
12	34.44681539192605	21.683810363549966	26.781297259598276	17.088076984925713
13	24.80811586766138	31.286238004983208	27.34064311900249	16.56500300835292
14	18.5188514415702	34.897074580050194	28.887867032607655	17.69620694577195
15	16.551090505349087	26.5389577186386	39.916642425184165	16.993309350828156
16	20.574965805958527	23.909088116734935	38.01207496851619	17.503871108790353
17	20.573023475993704	23.25754933969815	28.780361327578014	27.389065856730127
18	21.586242160665922	24.85423491170981	33.09757362333976	20.46194930428451
19	25.0444025664051	25.76229675549589	28.607358449316028	20.58594222878298
20	26.807586469819807	25.734607260881116	27.78213923705279	19.67566703224629
21	23.70324631092818	26.52116055570512	30.767952097626022	19.007641035740676
22	23.251406156553603	24.153279646730834	28.685187159301773	23.91012703741379
23	19.700781810396073	28.60528060795831	28.64082976336097	23.053107818284644
24	19.866241221120266	25.98033458666315	34.34798241604165	19.805441776174927
25	19.0120225707776	27.73904661411234	32.34458198348929	20.90434883162077
26	19.044048429965002	31.35521330396651	28.401064938866295	21.199673327202195
27	18.547308834078045	31.442889175169253	30.75837595919481	19.251426031557894
28	16.423709796028252	29.243042393384155	35.146189690654595	19.187058119933003
29	18.330535775911134	25.925181449755087	33.97523574465317	21.769047029680607
30	19.698207093931078	28.254938486861718	32.29200356304622	19.75485085616098
31	24.556968086163565	27.58722868360619	27.916882732054226	19.938920498176017
32	25.290446085437225	27.004213500909735	29.186398631154248	18.518941782498793
33	24.180833629952716	28.257332521469518	27.285851345808815	20.27598250276895
34	19.807519617532645	28.213788193886085	30.038087735496216	21.94060445308505
35	19.0959041229793	27.756121049617043	30.501627040124017	22.646347787279637
36	24.90681333215288	26.1343658699199	28.781761611971255	20.17705918595596
37	19.997145226656357	30.359746611763473	30.40550429209752	19.23760386948265
38	20.198695838354784	30.41060855456321	27.004529694159817	22.386165912922184
39	20.211479079751165	29.024462516645315	29.254967395958868	21.50909100764465
40	21.720579121488672	26.60499693744252	29.22239949119989	22.452024449868915
41	18.301581508296007	26.61389551890926	29.43533305990146	25.649189912893277
42	21.118502002858385	26.76788163170172	28.17819386801913	23.935422497420767
43	21.146869054437637	26.78351061234888	29.049532124330803	23.02008820888268
44	19.679822714961723	28.515436554469254	28.961856253128055	22.84288447744097
45	18.979093302304236	31.64272330922435	26.613534155194873	22.764649233276536
46	21.565870281267447	29.383838730601543	28.281227697083256	20.769063291047758
47	20.605365528431193	28.065583900523798	28.84427753455992	22.484773036485088
48	20.59660245835735	26.837218294399406	30.55167591456639	22.014503332676856
49	21.230524754317845	25.117488377639535	30.79889386567027	22.85309300237235
50	19.923110835671658	29.198775338371945	28.990223304707303	21.88789052124909
51	18.899954648853846	29.567140474723512	27.649925288052053	23.882979588370592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2046.0
1	3237.0
2	4428.0
3	10787.5
4	17147.0
5	12936.0
6	8725.0
7	8604.5
8	8484.0
9	9021.5
10	9559.0
11	9871.0
12	10183.0
13	10377.0
14	10571.0
15	10077.5
16	9584.0
17	9479.5
18	9375.0
19	8693.0
20	8011.0
21	9508.5
22	11006.0
23	11704.5
24	12403.0
25	13650.0
26	18947.5
27	22998.0
28	29623.0
29	36248.0
30	41874.5
31	47501.0
32	52860.0
33	58219.0
34	65226.5
35	72234.0
36	78507.5
37	84781.0
38	93751.0
39	102721.0
40	122628.5
41	142536.0
42	163534.0
43	184532.0
44	192811.5
45	201091.0
46	204808.5
47	208526.0
48	198298.0
49	188070.0
50	169557.0
51	151044.0
52	137856.0
53	124668.0
54	115153.5
55	105639.0
56	97767.5
57	89896.0
58	81480.0
59	73064.0
60	65446.5
61	57829.0
62	51066.5
63	44304.0
64	37632.0
65	30960.0
66	25527.5
67	20095.0
68	16259.5
69	12424.0
70	9937.5
71	7451.0
72	6055.5
73	4660.0
74	3888.0
75	2342.5
76	1569.0
77	1121.0
78	673.0
79	447.5
80	222.0
81	188.5
82	155.0
83	132.0
84	109.0
85	70.5
86	32.0
87	28.5
88	25.0
89	15.5
90	6.0
91	5.5
92	5.0
93	8.0
94	11.0
95	7.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2213836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.591656304838175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5883650197252	15.004929244159468
2	9.173701900194681	3.594560293433101
3	3.266478671692087	1.919871823886271
4	1.562196737003608	1.2242408620765743
5	0.9405897924768378	0.9213855969022635
6	0.6066767025675647	0.7131480866913756
7	0.4390920887860216	0.6021778902768455
8	0.3296620308243474	0.5166900163732466
9	0.28905200805037456	0.509670683635163
>10	3.5645099989193727	17.914374220720738
>50	2.380981080718056	34.0645615560052
>100	0.8526138921857827	21.582994371696348
>500	0.004910831306803153	0.6018705235535213
>1k	9.353964393910768E-4	0.3751783297084408
>5k	2.338491098477692E-4	0.4543465008815004
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9917	0.44795549444493626	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3938	0.17788128840618725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07331166355592736	0.0
2	0.0	0.0	0.0	0.26673159168068455	0.0
3	0.0	0.0	0.0	0.40427565546860744	0.0
4	0.0	0.0	0.0	0.622494168493059	0.0
5	0.0	0.0	0.0	1.0347649961424423	0.0
6	0.0	0.0	0.0	1.4188494540697685	0.0
7	0.0	0.0	0.0	1.6453793325250832	0.0
8	0.0	0.0	0.0	2.183359562316269	0.0
9	0.0	0.0	0.0	2.3591630093647407	0.0
10	0.0	0.0	0.0	2.742163376148911	0.0
11	0.0	0.0	0.0	3.291752415264726	0.0
12	0.0	0.0	0.0	3.7293638733853816	0.0
13	0.0	0.0	0.0	3.9023215811830685	0.0
14	0.0	0.0	0.0	3.9765366540249594	0.0
15	0.0	0.0	0.0	4.095199463736248	0.0
16	0.0	0.0	0.0	4.364099237703244	0.0
17	0.0	0.0	0.0	4.6704453265734225	0.0
18	0.0	0.0	0.0	5.057420694215832	0.0
19	0.0	0.0	0.0	5.270534944774591	0.0
20	0.0	0.0	0.0	5.509441530447603	0.0
21	0.0	0.0	0.0	5.80670835599385	0.0
22	0.0	0.0	0.0	6.120191378223138	0.0
23	0.0	0.0	0.0	6.440224117775662	0.0
24	0.0	0.0	0.0	6.682518488271037	0.0
25	0.0	0.0	0.0	6.900511148973998	0.0
26	0.0	0.0	0.0	7.123066026571074	0.0
27	0.0	0.0	0.0	7.333605560664837	0.0
28	0.0	0.0	0.0	7.563432883013918	0.0
29	0.0	0.0	0.0	7.805953105830784	0.0
30	0.0	0.0	0.0	8.105975329699218	0.0
31	0.0	0.0	0.0	8.391362323135047	0.0
32	0.0	0.0	0.0	8.635147318952262	0.0
33	0.0	0.0	0.0	8.890405612701212	0.0
34	0.0	0.0	0.0	9.143450553699552	0.0
35	0.0	0.0	0.0	9.460727894929887	0.0
36	0.0	0.0	0.0	9.743133637722035	0.0
37	0.0	0.0	0.0	10.01578256022578	0.0
38	0.0	0.0	0.0	10.28558574347874	0.0
39	0.0	0.0	0.0	10.59360313952795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCG	25	3.8919803E-5	45.000004	1
TCCGCAT	40	6.8193913E-9	45.0	36
CTATCGA	20	7.034704E-4	45.0	27
CGTTGTA	20	7.034704E-4	45.0	34
TCTATCG	35	1.2126111E-7	45.0	26
TAACGCC	60	0.0	44.999996	12
CACTCGA	30	2.1664182E-6	44.999996	27
CGACGGT	675	0.0	43.333336	28
TACGGGA	850	0.0	41.823532	4
CGGTCTA	710	0.0	40.880283	31
CGTTTTT	5915	0.0	40.663567	1
AGGGCGA	1840	0.0	40.475548	6
GGCGATA	830	0.0	40.391563	8
TAGGGCG	720	0.0	40.3125	5
CGTTAAC	45	1.9297659E-8	40.0	20
GACGATT	45	1.9297659E-8	40.0	9
GATCGAC	80	0.0	39.375	9
CACGACG	745	0.0	38.959732	26
GACGGTC	750	0.0	38.7	29
GGGCGAT	3640	0.0	38.695057	7
>>END_MODULE