FastQCFastQC Report
Thu 26 May 2016
SRR1547958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547958_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2699161
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144970.5370928225474508No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38790.14371132362982422No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG207.034979E-445.01
CGCACGA207.034979E-445.020
TCGCGTG207.034979E-445.01
CGACGGT4000.043.312528
GCTACGA850.042.3529410
CGGTCTA4150.042.28915431
CGTTTTT77650.041.493881
GCGCGAC2600.040.6730779
CGTAAGG4050.040.02
TCGCGAG1300.039.807691
CTACGGG4700.039.7340433
CGCGAGG3800.039.671052
GACGTAC403.4605364E-739.3759
TACGACG403.4605364E-739.3751
ACGCGGT403.4605364E-739.37523
ACGGTCT4400.038.86363630
AGGGATC40150.038.6674966
AGGGATT64800.038.611116
ACGGGAT14400.038.593755
CGGTCGT356.2514227E-638.57142626
Produced by FastQC (version 0.11.5)