##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547958_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2699161
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.391272695478335	31.0	31.0	33.0	30.0	34.0
2	31.783696118905098	31.0	31.0	34.0	30.0	34.0
3	31.87906056733926	31.0	31.0	34.0	30.0	34.0
4	35.64804433674019	37.0	35.0	37.0	33.0	37.0
5	35.56400340698462	37.0	35.0	37.0	33.0	37.0
6	35.627884738998524	37.0	35.0	37.0	33.0	37.0
7	35.967853714543146	37.0	35.0	37.0	35.0	37.0
8	35.97949362783472	37.0	35.0	37.0	35.0	37.0
9	37.76309342051104	39.0	38.0	39.0	35.0	39.0
10	37.21880984498517	39.0	37.0	39.0	33.0	39.0
11	36.94753110318354	39.0	37.0	39.0	33.0	39.0
12	36.47223489076791	39.0	35.0	39.0	32.0	39.0
13	36.307876039999094	39.0	35.0	39.0	32.0	39.0
14	37.365112714654664	39.0	35.0	41.0	32.0	41.0
15	37.562924182736786	40.0	35.0	41.0	32.0	41.0
16	37.63667858271515	40.0	35.0	41.0	33.0	41.0
17	37.5694599173595	39.0	35.0	41.0	33.0	41.0
18	37.481812681792604	39.0	36.0	41.0	32.0	41.0
19	37.43345728543055	39.0	36.0	41.0	32.0	41.0
20	37.28210803282946	39.0	35.0	41.0	32.0	41.0
21	37.17027031733194	39.0	35.0	41.0	32.0	41.0
22	37.09804972730415	39.0	35.0	41.0	32.0	41.0
23	36.96525364733708	39.0	35.0	41.0	32.0	41.0
24	36.943494663712166	39.0	35.0	41.0	32.0	41.0
25	36.96755991954537	39.0	35.0	41.0	32.0	41.0
26	36.88640433082725	39.0	35.0	41.0	32.0	41.0
27	36.817613695515014	39.0	35.0	41.0	31.0	41.0
28	36.776643927501915	39.0	35.0	41.0	31.0	41.0
29	36.77615340470613	38.0	35.0	41.0	31.0	41.0
30	36.71615105582809	38.0	35.0	41.0	31.0	41.0
31	36.57417545674378	38.0	35.0	41.0	31.0	41.0
32	36.42773032064408	38.0	35.0	41.0	30.0	41.0
33	36.31439250937606	38.0	35.0	41.0	30.0	41.0
34	36.17744588040506	38.0	35.0	41.0	30.0	41.0
35	36.10193426772245	38.0	35.0	41.0	30.0	41.0
36	36.014005092693615	38.0	35.0	41.0	30.0	41.0
37	35.92883232974987	38.0	35.0	41.0	29.0	41.0
38	35.83125904679269	38.0	35.0	40.0	29.0	41.0
39	35.76646891385879	38.0	35.0	40.0	29.0	41.0
40	35.61575800776612	38.0	35.0	40.0	27.0	41.0
41	35.528730594432865	38.0	35.0	40.0	27.0	41.0
42	35.52388834900919	38.0	35.0	40.0	27.0	41.0
43	35.46168049997758	38.0	35.0	40.0	27.0	41.0
44	35.309679563390254	38.0	34.0	40.0	27.0	41.0
45	35.20737110531754	38.0	34.0	40.0	27.0	41.0
46	35.17908083289585	38.0	34.0	40.0	26.0	41.0
47	35.12860737095712	38.0	34.0	40.0	26.0	41.0
48	35.03574110621782	38.0	34.0	40.0	26.0	41.0
49	34.904670006716906	37.0	34.0	40.0	26.0	41.0
50	34.80305102215096	37.0	34.0	40.0	25.0	41.0
51	34.48213982048496	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	5.0
13	12.0
14	19.0
15	63.0
16	141.0
17	372.0
18	898.0
19	1707.0
20	3035.0
21	4966.0
22	7953.0
23	11924.0
24	17949.0
25	26772.0
26	35517.0
27	40909.0
28	45092.0
29	51535.0
30	61087.0
31	75439.0
32	94241.0
33	122039.0
34	200941.0
35	291901.0
36	195626.0
37	254278.0
38	403981.0
39	750635.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.61440832910671	24.023465069330804	29.55674003884911	12.805386562713378
2	30.461169229994063	25.716880171282853	30.447313072469555	13.374637526253528
3	29.219894626515426	25.674570727718727	31.771391184149444	13.334143461616407
4	25.168042958534155	28.357552587637418	31.704444455147357	14.76995999868107
5	23.581549970527877	33.27593278059368	29.39754242151543	13.744974827363022
6	21.739496087858413	41.90505864600148	26.085698481861584	10.26974678427852
7	89.6328525790051	3.1371970771658306	5.894831764389008	1.3351185794400555
8	90.14704939794255	2.5466431976454906	5.5961463580720086	1.710161046339955
9	85.57740720171935	5.4880016419917155	6.766732329045952	2.167858827242984
10	38.33457878207339	41.60433556946029	11.297769936658094	8.763315711808223
11	32.14246945624955	24.92533791055813	27.629511540808423	15.302681092383894
12	31.114261061122328	23.920951732779187	28.12988925077089	16.834897955327598
13	23.401864505303685	31.72419133204725	28.806321668103536	16.067622494545528
14	17.484655416998095	35.049483895180764	29.834048432086856	17.631812255734282
15	16.1683945492692	28.23799691830165	39.356377778131794	16.237230754297354
16	19.787296867433994	26.494640371582136	37.39413840078454	16.323924360199335
17	19.628729075442333	25.41074800651017	29.525434014495616	25.435088903551883
18	21.492122922641517	26.99901932489392	32.53277592555613	18.976081826908437
19	24.392320428459065	27.930123471701023	28.869193056657235	18.808363043182677
20	26.746607556940845	28.110327616618648	27.57434625055712	17.568718575883395
21	22.738250886108684	28.691878698603013	30.832173404995107	17.7376970102932
22	21.949450218049236	25.04441194875	29.89380774247998	23.112330090720786
23	18.24815192572803	29.672294464835552	30.202125771674975	21.877427837761438
24	19.023207581911564	27.386065521841786	35.22498287430798	18.365744021938667
25	18.65568597056641	28.978819714718757	32.66889229653214	19.696602018182688
26	18.819514656591437	33.23191910375113	28.422646889162966	19.52591935049447
27	18.09525256181458	32.737395064614525	30.918867010897088	18.248485362673808
28	16.67306989097723	30.08831262751648	35.0297740668304	18.208843414675894
29	17.58798382163939	27.950500173942938	33.78868470609942	20.672831298318258
30	18.97867522537559	30.587912317938798	31.932589423157786	18.500823033527823
31	23.339956379037783	28.984673385544617	28.769384264221365	18.905985971196234
32	23.989862034906402	29.80959638939656	28.82469774867079	17.375843827026248
33	23.47907368252579	29.90532984138404	27.99807051154044	18.617525964549724
34	19.177181353761412	30.24617649706705	30.084422529815747	20.492219619355794
35	19.37235311268946	29.630911235009695	30.08312583058217	20.913609821718676
36	23.728743857813594	29.17936351332877	28.613372822147326	18.47851980671031
37	19.886068300483	31.672249265605128	29.830936353926273	18.610746079985596
38	19.509284551755158	32.0664087840629	27.878996473348572	20.545310190833373
39	20.07675718491783	30.7199162999169	28.674873414368392	20.528453100796877
40	22.099607989297414	28.817880815557128	28.478034470711457	20.604476724434
41	18.284237212970993	28.59432986768852	29.060734057731274	24.060698861609218
42	20.77804917898562	28.962925886970066	27.691975395317286	22.567049538727034
43	20.741667503346413	28.466401226158794	29.108860123571734	21.683071146923062
44	19.480460780220223	30.97481032068854	29.223969966963807	20.32075893212743
45	18.661836029788514	33.11503092998158	27.077969783943974	21.145163256285933
46	20.706730721138904	31.344777136302724	28.223029304291224	19.72546283826715
47	19.802746112588316	29.97298049282722	29.02561203277611	21.198661361808355
48	20.766638225730144	28.584956584657235	30.159705182462254	20.48870000715037
49	20.86911451373223	27.07819207524116	30.937428334212	21.11526507681461
50	19.19500170608571	31.212106280433066	28.918912210127516	20.67397980335371
51	18.273085599562233	31.730193197071237	27.549931256416343	22.446789946950183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2241.0
1	3356.0
2	4471.0
3	13918.5
4	23366.0
5	17569.0
6	11772.0
7	11533.0
8	11294.0
9	12111.0
10	12928.0
11	14185.0
12	15442.0
13	15173.0
14	14904.0
15	14782.0
16	14660.0
17	13917.0
18	13174.0
19	13268.0
20	13362.0
21	14441.0
22	15520.0
23	17241.0
24	18962.0
25	22472.5
26	30688.5
27	35394.0
28	41899.0
29	48404.0
30	57886.0
31	67368.0
32	77502.0
33	87636.0
34	95948.0
35	104260.0
36	115822.0
37	127384.0
38	137669.0
39	147954.0
40	168132.5
41	188311.0
42	212638.5
43	236966.0
44	245398.0
45	253830.0
46	253587.0
47	253344.0
48	232376.5
49	211409.0
50	195562.0
51	179715.0
52	161035.0
53	142355.0
54	127787.0
55	113219.0
56	103327.5
57	93436.0
58	83390.0
59	73344.0
60	63341.5
61	53339.0
62	44005.5
63	34672.0
64	28645.5
65	22619.0
66	17464.5
67	12310.0
68	9001.0
69	5692.0
70	4643.0
71	3594.0
72	2877.5
73	2161.0
74	1621.0
75	821.5
76	562.0
77	417.5
78	273.0
79	281.0
80	289.0
81	173.5
82	58.0
83	55.0
84	52.0
85	34.0
86	16.0
87	16.5
88	17.0
89	12.5
90	8.0
91	8.5
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2699161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.16502117439188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85814649353662	15.296811302922869
2	9.769457752737846	3.9400264489257117
3	3.4800977762401533	2.1052873604051037
4	1.7775417248220275	1.43376666077605
5	1.0487605042301447	1.0574138887333384
6	0.7044770761113878	0.8523477093995889
7	0.4870331334583208	0.6874723414172226
8	0.37698340506769945	0.6081502676467608
9	0.2927983698430505	0.5313856794941285
>10	3.287668026734958	16.276585911335783
>50	1.8739031660539054	27.812536164469975
>100	1.0369690507367073	27.83697669979885
>500	0.00504288034957304	0.6569160371277605
>1k	9.338667314024149E-4	0.3583246862305649
>5k	0.0	0.0
>10k+	1.8677334628048296E-4	0.5459988413164283
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14497	0.5370928225474508	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3879	0.14371132362982422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7048549530761595E-5	0.0	0.0	0.06650214640771707	0.0
2	3.7048549530761595E-5	0.0	0.0	0.2197719958164778	0.0
3	3.7048549530761595E-5	0.0	0.0	0.3292134111303475	0.0
4	3.7048549530761595E-5	0.0	0.0	0.5301647437851984	0.0
5	3.7048549530761595E-5	0.0	0.0	0.9197672906506874	0.0
6	3.7048549530761595E-5	0.0	0.0	1.3245597428237885	0.0
7	3.7048549530761595E-5	0.0	0.0	1.543924204595428	0.0
8	3.7048549530761595E-5	0.0	0.0	2.093984019478645	0.0
9	3.7048549530761595E-5	0.0	0.0	2.2822647481939757	0.0
10	3.7048549530761595E-5	0.0	0.0	2.6518981268623842	0.0
11	3.7048549530761595E-5	0.0	0.0	3.1732823644087924	0.0
12	3.7048549530761595E-5	0.0	0.0	3.5816314773368467	0.0
13	3.7048549530761595E-5	0.0	0.0	3.7503876204494655	0.0
14	3.7048549530761595E-5	0.0	0.0	3.820557573260728	0.0
15	3.7048549530761595E-5	0.0	0.0	3.9365936303910734	0.0
16	3.7048549530761595E-5	0.0	0.0	4.190598485973974	0.0
17	3.7048549530761595E-5	0.0	0.0	4.520330576797753	0.0
18	3.7048549530761595E-5	0.0	0.0	4.909081007024034	0.0
19	3.7048549530761595E-5	0.0	0.0	5.137633509079302	0.0
20	3.7048549530761595E-5	0.0	0.0	5.378967760722684	0.0
21	3.7048549530761595E-5	0.0	0.0	5.679135109020915	0.0
22	3.7048549530761595E-5	0.0	0.0	6.0284288339969345	0.0
23	3.7048549530761595E-5	0.0	0.0	6.399395960448451	0.0
24	3.7048549530761595E-5	0.0	0.0	6.664293089593396	0.0
25	3.7048549530761595E-5	0.0	0.0	6.889807610587142	0.0
26	3.7048549530761595E-5	0.0	0.0	7.116507685165872	0.0
27	3.7048549530761595E-5	0.0	0.0	7.344949041572548	0.0
28	3.7048549530761595E-5	0.0	0.0	7.599324382650757	0.0
29	3.7048549530761595E-5	0.0	0.0	7.873965280322293	0.0
30	3.7048549530761595E-5	0.0	0.0	8.211440517998	0.0
31	3.7048549530761595E-5	0.0	0.0	8.517646779869745	0.0
32	3.7048549530761595E-5	0.0	0.0	8.799438047600717	0.0
33	3.7048549530761595E-5	0.0	0.0	9.073300925732108	0.0
34	3.7048549530761595E-5	0.0	0.0	9.370096856023038	0.0
35	3.7048549530761595E-5	0.0	0.0	9.705608520573616	0.0
36	3.7048549530761595E-5	0.0	0.0	10.017001579379667	0.0
37	3.7048549530761595E-5	0.0	0.0	10.330506405508972	0.0
38	3.7048549530761595E-5	0.0	0.0	10.658867700000112	0.0
39	3.7048549530761595E-5	0.0	0.0	11.065327336902097	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACCG	20	7.034979E-4	45.0	1
CGCACGA	20	7.034979E-4	45.0	20
TCGCGTG	20	7.034979E-4	45.0	1
CGACGGT	400	0.0	43.3125	28
GCTACGA	85	0.0	42.35294	10
CGGTCTA	415	0.0	42.289154	31
CGTTTTT	7765	0.0	41.49388	1
GCGCGAC	260	0.0	40.673077	9
CGTAAGG	405	0.0	40.0	2
TCGCGAG	130	0.0	39.80769	1
CTACGGG	470	0.0	39.734043	3
CGCGAGG	380	0.0	39.67105	2
GACGTAC	40	3.4605364E-7	39.375	9
TACGACG	40	3.4605364E-7	39.375	1
ACGCGGT	40	3.4605364E-7	39.375	23
ACGGTCT	440	0.0	38.863636	30
AGGGATC	4015	0.0	38.667496	6
AGGGATT	6480	0.0	38.61111	6
ACGGGAT	1440	0.0	38.59375	5
CGGTCGT	35	6.2514227E-6	38.571426	26
>>END_MODULE