FastQCFastQC Report
Thu 26 May 2016
SRR1547957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547957_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2997942
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126000.4202883177860012No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39390.13139013363167135No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC32550.10857448209471697TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG32040.10687331509415458No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT32020.10680660266275997Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA207.035104E-445.038
TCACGAC9700.042.91237325
CGACGGT9600.042.65624628
CGGTCTA9700.041.7525831
GGCGATA9100.040.7967038
CTCACGA10500.039.85714324
CGTTTTT74300.039.821671
GCGATAC1300.039.8076939
ATAGCGG4250.039.7058832
CGTAAGG3750.039.6000022
CTATGCG1650.039.5454521
TATAGCG2400.039.3749961
GCGATAT3350.038.9552239
TTAGGGA51200.038.8476564
AGGGTAA14800.038.766896
TCGGGTA1050.038.5714265
GGGCGAT41250.038.47
CGCATCG1350.038.33333621
GACGGTC10700.038.27102729
TAAGGGA45850.038.2279174
Produced by FastQC (version 0.11.5)