##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547957_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2997942
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34480420234948	31.0	31.0	33.0	30.0	34.0
2	31.746707574729598	31.0	31.0	34.0	30.0	34.0
3	31.86228352649918	31.0	31.0	34.0	30.0	34.0
4	35.64187099016592	37.0	35.0	37.0	33.0	37.0
5	35.5374200034557	37.0	35.0	37.0	33.0	37.0
6	35.575187245116815	37.0	35.0	37.0	33.0	37.0
7	35.901412368885055	37.0	35.0	37.0	35.0	37.0
8	35.90184900174853	37.0	35.0	37.0	35.0	37.0
9	37.63428945590008	39.0	37.0	39.0	35.0	39.0
10	37.130936822660345	39.0	37.0	39.0	33.0	39.0
11	36.937388048201065	39.0	37.0	39.0	33.0	39.0
12	36.35550454278302	38.0	35.0	39.0	32.0	39.0
13	36.13126004439045	38.0	35.0	39.0	32.0	39.0
14	36.98169244101454	39.0	35.0	41.0	31.0	41.0
15	37.221559323028934	39.0	35.0	41.0	32.0	41.0
16	37.357748081850815	39.0	35.0	41.0	32.0	41.0
17	37.322012900849984	39.0	35.0	41.0	32.0	41.0
18	37.25269835106884	39.0	35.0	41.0	32.0	41.0
19	37.194623178166886	39.0	35.0	41.0	32.0	41.0
20	37.04581209376299	39.0	35.0	41.0	32.0	41.0
21	36.93951917682197	39.0	35.0	41.0	31.0	41.0
22	36.90991420114198	39.0	35.0	41.0	31.0	41.0
23	36.78242541049827	38.0	35.0	41.0	31.0	41.0
24	36.74624592470435	38.0	35.0	41.0	31.0	41.0
25	36.772060300032486	38.0	35.0	41.0	31.0	41.0
26	36.67801244987395	38.0	35.0	41.0	31.0	41.0
27	36.62819460816787	38.0	35.0	41.0	31.0	41.0
28	36.56832353661278	38.0	35.0	41.0	31.0	41.0
29	36.545821433503384	38.0	35.0	40.0	31.0	41.0
30	36.477163334047155	38.0	35.0	40.0	31.0	41.0
31	36.31239530317798	38.0	35.0	40.0	30.0	41.0
32	36.23603758845235	38.0	35.0	40.0	30.0	41.0
33	36.12745109811998	38.0	35.0	40.0	30.0	41.0
34	35.975196318007484	38.0	35.0	40.0	29.0	41.0
35	35.90909630673308	38.0	35.0	40.0	29.0	41.0
36	35.82062027884462	38.0	35.0	40.0	29.0	41.0
37	35.72401467406641	38.0	35.0	40.0	29.0	41.0
38	35.580260725524376	38.0	34.0	40.0	28.0	41.0
39	35.49958304730379	38.0	34.0	40.0	27.0	41.0
40	35.29673789552967	38.0	34.0	40.0	26.0	41.0
41	35.153565012265084	38.0	34.0	40.0	26.0	41.0
42	35.17947845555384	38.0	34.0	40.0	26.0	41.0
43	35.1464551348892	38.0	34.0	40.0	26.0	41.0
44	35.01761441682327	38.0	34.0	40.0	26.0	41.0
45	34.91540596849439	37.0	34.0	40.0	25.0	41.0
46	34.86360243126785	37.0	34.0	40.0	25.0	41.0
47	34.77635091005763	37.0	34.0	40.0	25.0	41.0
48	34.63218234375448	37.0	34.0	40.0	24.0	41.0
49	34.517845575398056	37.0	34.0	40.0	24.0	41.0
50	34.39851371374096	37.0	33.0	40.0	24.0	41.0
51	34.06597592615201	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	4.0
11	2.0
12	6.0
13	11.0
14	39.0
15	101.0
16	211.0
17	656.0
18	1253.0
19	2523.0
20	4209.0
21	6873.0
22	10324.0
23	15368.0
24	22189.0
25	31208.0
26	40681.0
27	47581.0
28	53874.0
29	62195.0
30	74533.0
31	91214.0
32	113702.0
33	147141.0
34	239561.0
35	316697.0
36	223609.0
37	289652.0
38	445474.0
39	756936.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.54809465960315	24.451740560691302	29.778494714040498	13.221670065665045
2	30.855833768631946	26.657120117734102	29.279252233698983	13.207793879934968
3	28.243808586023345	26.347441011200353	31.276922635594683	14.13182776718162
4	26.182127606204524	28.06401858341489	30.85836884102494	14.895484969355643
5	23.975780718906503	31.43716589580452	29.272347497049644	15.314705888239333
6	22.51104257520659	40.60211972079513	26.19270152658057	10.694136177417708
7	87.79039087480679	3.3587707834240956	6.8717139958011195	1.9791243459680006
8	87.69655984005027	2.99935755928567	6.416901994768411	2.887180605895644
9	81.71128727640495	6.198685631676663	8.368340681707652	3.721686410210738
10	36.58042750660287	39.48892273432909	12.96609474099232	10.964555018075734
11	36.11737652029292	22.64963765142888	25.40165887131906	15.83132695695914
12	33.800220284448464	21.076091532124373	27.374879167108634	17.74880901631853
13	25.74446070004023	28.334370711641522	29.151531283794014	16.769637304524238
14	18.098448869257645	31.849181872097592	31.23886319348406	18.813506065160702
15	17.22738465253831	25.9907963529648	39.435619501644794	17.346199492852097
16	22.186986939707307	24.579961853831726	35.210220878189105	18.022830328271862
17	22.21263786957853	24.004366995759092	27.4816524135557	26.30134272110668
18	22.768485847958367	24.945045634638696	32.09228197209953	20.19418654530341
19	25.13637688787842	26.5483454983452	27.30256289147689	21.012714722299496
20	25.05812320585255	28.04223697456455	27.201993901149525	19.69764591843338
21	23.17749976483868	26.415287553928664	31.560984168472906	18.84622851275975
22	22.328950993715022	22.735263057123852	30.594287681349407	24.34149826781172
23	19.230625542455456	28.06241748506142	30.07369722296162	22.633259749521507
24	20.3438892413529	26.202775103721155	33.15677888364751	20.296556771278432
25	21.12792709131798	27.015799505127188	30.299852365389324	21.556421038165517
26	19.122784897106083	29.98400235895157	27.91411575007122	22.979096993871128
27	18.842592685248746	29.440229330654162	30.821977209699185	20.895200774397903
28	16.934650503578787	27.97785947826876	33.590176194202556	21.497313823949895
29	18.39948871592579	26.358281781301972	33.06494922183285	22.17728028093939
30	21.082462569322555	25.99092977782759	31.962025949801564	20.96458170304829
31	23.747057147870105	26.14890481537001	29.113204991957815	20.990833044802066
32	25.436749610232617	25.765241622419648	28.41042288343137	20.38758588391637
33	24.12451608470077	25.557532467272548	28.338073251583918	21.97987819644276
34	18.97951995068617	25.83308816514796	31.948550038659857	23.238841845506016
35	19.91976495876171	26.096101925921182	31.46685292777512	22.51728018754199
36	25.137177437055154	24.515017301869083	28.789649699693992	21.558155561381774
37	19.718493553244194	28.706159091803645	29.9675911008285	21.60775625412366
38	21.358518610433425	28.011916174495706	27.217604610095858	23.411960604975015
39	20.120569377259468	26.33409852492143	28.811197815034443	24.734134282784655
40	23.68311328237838	24.142828647118588	29.11970945401879	23.05434861648424
41	19.092964440272695	24.365648167976563	29.757913928955265	26.783473462795477
42	21.255181054203184	24.797310955315346	29.068741156433315	24.878766834048157
43	20.505033119386564	24.288128322696036	30.614401479414877	24.59243707850252
44	20.78979513279443	26.36401905040191	29.306037274903918	23.540148541899743
45	19.296704205751812	27.717947845555386	27.671649418167533	25.313698530525276
46	21.365456703298463	26.809324529960886	28.821104611096544	23.004114155644107
47	19.963828519697845	25.98622655141427	30.756198752344112	23.293746176543774
48	20.32077338387467	25.887125234577585	30.705163742327237	23.086937639220505
49	21.51862844578047	23.415763213564507	30.857601648063905	24.208006692591116
50	19.796680522838667	26.500045698015505	30.123965040017453	23.579308739128376
51	18.81810922292693	27.057294637454625	28.150444538286596	25.974151601331847
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2321.0
1	3677.0
2	5033.0
3	13156.5
4	21280.0
5	15886.0
6	10492.0
7	10295.5
8	10099.0
9	10792.0
10	11485.0
11	11902.5
12	12320.0
13	12364.5
14	12409.0
15	12239.5
16	12070.0
17	11834.5
18	11599.0
19	11719.0
20	11839.0
21	12232.5
22	12626.0
23	14794.0
24	16962.0
25	18015.5
26	22043.5
27	25018.0
28	31522.0
29	38026.0
30	48138.5
31	58251.0
32	63503.5
33	68756.0
34	78372.5
35	87989.0
36	90549.5
37	93110.0
38	108573.5
39	124037.0
40	146161.0
41	168285.0
42	191630.5
43	214976.0
44	233922.0
45	252868.0
46	269554.5
47	286241.0
48	289662.0
49	293083.0
50	272087.5
51	251092.0
52	225215.5
53	199339.0
54	179894.5
55	160450.0
56	149113.5
57	137777.0
58	126862.0
59	115947.0
60	101347.0
61	86747.0
62	73482.5
63	60218.0
64	51170.0
65	42122.0
66	33848.5
67	25575.0
68	20014.0
69	14453.0
70	12375.0
71	10297.0
72	7825.0
73	5353.0
74	4616.0
75	3054.5
76	2230.0
77	1640.5
78	1051.0
79	839.0
80	627.0
81	461.0
82	295.0
83	217.5
84	140.0
85	91.0
86	42.0
87	36.0
88	30.0
89	22.0
90	14.0
91	8.0
92	2.0
93	4.0
94	6.0
95	4.5
96	3.0
97	3.0
98	3.0
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2997942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.263395122406887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64263395171098	15.87155818692414
2	10.407152729203723	4.42582801160588
3	3.8270466638458243	2.4412801609572856
4	1.8974696188276872	1.6138658495158362
5	1.1070974380095027	1.1770325131700212
6	0.7580955372832463	0.9671810969872207
7	0.5309095429523734	0.7902257570236937
8	0.41546280218580794	0.7067319777231365
9	0.3179018696104406	0.6083705757310807
>10	3.4130802720381817	17.626928086401076
>50	1.9092763936516273	29.81085737327824
>100	0.764649547673196	21.364585142326362
>500	0.005725013590793467	0.8128736170302803
>1k	0.0033395912612961894	1.3566153601080666
>5k	0.0	0.0
>10k+	1.5902815529981855E-4	0.4260662912176684
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12600	0.4202883177860012	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3939	0.13139013363167135	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	3255	0.10857448209471697	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3204	0.10687331509415458	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	3202	0.10680660266275997	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3356215697301685E-5	0.0	0.0	0.1287549925915845	6.671243139460337E-5
2	3.3356215697301685E-5	0.0	0.0	0.4661864705854883	6.671243139460337E-5
3	3.3356215697301685E-5	0.0	0.0	0.7352043501842264	6.671243139460337E-5
4	3.3356215697301685E-5	0.0	0.0	1.1754730411729113	6.671243139460337E-5
5	3.3356215697301685E-5	0.0	0.0	2.0462370519509716	6.671243139460337E-5
6	3.3356215697301685E-5	0.0	0.0	2.84842068325538	6.671243139460337E-5
7	3.3356215697301685E-5	0.0	0.0	3.2945934244224873	6.671243139460337E-5
8	3.3356215697301685E-5	0.0	0.0	4.263558134213404	6.671243139460337E-5
9	3.3356215697301685E-5	0.0	0.0	4.593384395028322	6.671243139460337E-5
10	3.3356215697301685E-5	0.0	0.0	5.328922307369522	6.671243139460337E-5
11	3.3356215697301685E-5	0.0	0.0	6.212128186602676	6.671243139460337E-5
12	3.3356215697301685E-5	0.0	0.0	6.986526090231232	6.671243139460337E-5
13	3.3356215697301685E-5	0.0	0.0	7.291702107645845	6.671243139460337E-5
14	3.3356215697301685E-5	0.0	0.0	7.427061630945495	6.671243139460337E-5
15	3.3356215697301685E-5	0.0	0.0	7.6325359196408735	6.671243139460337E-5
16	3.3356215697301685E-5	0.0	0.0	8.036246198225315	6.671243139460337E-5
17	3.3356215697301685E-5	0.0	0.0	8.511538915696168	6.671243139460337E-5
18	3.3356215697301685E-5	0.0	0.0	9.09537275904604	6.671243139460337E-5
19	3.3356215697301685E-5	0.0	0.0	9.407953856345452	6.671243139460337E-5
20	3.3356215697301685E-5	0.0	0.0	9.77613976521227	6.671243139460337E-5
21	3.3356215697301685E-5	0.0	0.0	10.204033300177255	6.671243139460337E-5
22	3.3356215697301685E-5	0.0	0.0	10.66184736062272	6.671243139460337E-5
23	3.3356215697301685E-5	0.0	0.0	11.128100543639603	6.671243139460337E-5
24	3.3356215697301685E-5	0.0	0.0	11.454657895316187	6.671243139460337E-5
25	3.3356215697301685E-5	0.0	0.0	11.750394103688464	6.671243139460337E-5
26	3.3356215697301685E-5	0.0	0.0	12.050499976317086	6.671243139460337E-5
27	3.3356215697301685E-5	0.0	0.0	12.396570714176592	6.671243139460337E-5
28	3.3356215697301685E-5	0.0	0.0	12.7166569600079	6.671243139460337E-5
29	3.3356215697301685E-5	0.0	0.0	13.083875538619493	6.671243139460337E-5
30	3.3356215697301685E-5	0.0	0.0	13.50953420713276	6.671243139460337E-5
31	3.3356215697301685E-5	0.0	0.0	13.88299039807975	6.671243139460337E-5
32	3.3356215697301685E-5	3.3356215697301685E-5	0.0	14.20858041950111	1.0006864709190505E-4
33	3.3356215697301685E-5	3.3356215697301685E-5	0.0	14.540908396493327	1.0006864709190505E-4
34	3.3356215697301685E-5	3.3356215697301685E-5	0.0	14.91346396961649	1.0006864709190505E-4
35	3.3356215697301685E-5	3.3356215697301685E-5	0.0	15.321810762182858	1.0006864709190505E-4
36	3.3356215697301685E-5	3.3356215697301685E-5	0.0	15.662177587158125	1.0006864709190505E-4
37	3.3356215697301685E-5	3.3356215697301685E-5	0.0	16.028929178749955	1.0006864709190505E-4
38	3.3356215697301685E-5	3.3356215697301685E-5	0.0	16.43744275239481	1.0006864709190505E-4
39	3.3356215697301685E-5	3.3356215697301685E-5	0.0	17.14886412078686	1.0006864709190505E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	20	7.035104E-4	45.0	38
TCACGAC	970	0.0	42.912373	25
CGACGGT	960	0.0	42.656246	28
CGGTCTA	970	0.0	41.75258	31
GGCGATA	910	0.0	40.796703	8
CTCACGA	1050	0.0	39.857143	24
CGTTTTT	7430	0.0	39.82167	1
GCGATAC	130	0.0	39.807693	9
ATAGCGG	425	0.0	39.705883	2
CGTAAGG	375	0.0	39.600002	2
CTATGCG	165	0.0	39.545452	1
TATAGCG	240	0.0	39.374996	1
GCGATAT	335	0.0	38.955223	9
TTAGGGA	5120	0.0	38.847656	4
AGGGTAA	1480	0.0	38.76689	6
TCGGGTA	105	0.0	38.571426	5
GGGCGAT	4125	0.0	38.4	7
CGCATCG	135	0.0	38.333336	21
GACGGTC	1070	0.0	38.271027	29
TAAGGGA	4585	0.0	38.227917	4
>>END_MODULE