FastQCFastQC Report
Thu 26 May 2016
SRR1547956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547956_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2440206
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141280.578967513398459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA5200.041.53846431
CGTTTTT72900.040.9567871
CGACGGT5300.040.7547228
TACGTAG1250.039.61
CACGACG5400.039.58333226
GGGCGAT38850.039.498077
GTAATCG403.4603545E-739.3751
AGGGCGA19750.039.30386
ACACGAC5300.038.63207626
TACGGGA13300.038.571434
CGTTAGG3500.038.5714262
GGCGATA8300.038.4939738
TGGGCGA11550.038.181826
AAGGGAT45500.037.8791245
TATGGGA31900.037.8761754
ATAGGGA42000.037.8754
TAAGGGA42350.037.7744984
AGGGATT49100.037.530556
CACGACC5400.037.527
GCGATAT1800.037.59
Produced by FastQC (version 0.11.5)