##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547956_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2440206
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332352268619946	31.0	31.0	33.0	30.0	34.0
2	31.72355448679333	31.0	31.0	34.0	30.0	34.0
3	31.816375338803365	31.0	31.0	34.0	30.0	34.0
4	35.61357565713714	37.0	35.0	37.0	33.0	37.0
5	35.5301355705215	37.0	35.0	37.0	33.0	37.0
6	35.579003166126135	37.0	35.0	37.0	33.0	37.0
7	35.91267950328784	37.0	35.0	37.0	35.0	37.0
8	35.9111681554754	37.0	35.0	37.0	35.0	37.0
9	37.64611389366308	39.0	37.0	39.0	35.0	39.0
10	37.124584153960775	39.0	37.0	39.0	33.0	39.0
11	36.9382105445196	39.0	37.0	39.0	33.0	39.0
12	36.47577335683955	39.0	35.0	39.0	32.0	39.0
13	36.33482419107239	39.0	35.0	39.0	32.0	39.0
14	37.35608510101196	39.0	36.0	41.0	32.0	41.0
15	37.51594414569917	40.0	36.0	41.0	32.0	41.0
16	37.59342366996885	40.0	36.0	41.0	32.0	41.0
17	37.509463955092315	39.0	35.0	41.0	32.0	41.0
18	37.41348476317163	39.0	36.0	41.0	32.0	41.0
19	37.35384430658723	39.0	36.0	41.0	32.0	41.0
20	37.220788736688625	39.0	35.0	41.0	32.0	41.0
21	37.114828830025004	39.0	35.0	41.0	32.0	41.0
22	37.03948396159996	39.0	35.0	41.0	31.0	41.0
23	36.896285805378724	39.0	35.0	41.0	31.0	41.0
24	36.873904498226786	39.0	35.0	41.0	31.0	41.0
25	36.89522564898209	39.0	35.0	41.0	31.0	41.0
26	36.81383825791757	39.0	35.0	41.0	31.0	41.0
27	36.7363296377437	39.0	35.0	41.0	31.0	41.0
28	36.665742564357274	39.0	35.0	41.0	31.0	41.0
29	36.64737198416855	38.0	35.0	41.0	31.0	41.0
30	36.53925939039573	38.0	35.0	41.0	30.0	41.0
31	36.41891299341121	38.0	35.0	41.0	30.0	41.0
32	36.267752804476345	38.0	35.0	41.0	30.0	41.0
33	36.139498468571915	38.0	35.0	41.0	30.0	41.0
34	35.99190560141234	38.0	35.0	41.0	29.0	41.0
35	35.88335697887801	38.0	35.0	41.0	29.0	41.0
36	35.80834978686226	38.0	35.0	40.0	28.0	41.0
37	35.71263204827789	38.0	35.0	40.0	28.0	41.0
38	35.58679636063513	38.0	34.0	40.0	27.0	41.0
39	35.518561957474084	38.0	34.0	40.0	27.0	41.0
40	35.36896884935124	38.0	34.0	40.0	26.0	41.0
41	35.250159207870155	38.0	34.0	40.0	26.0	41.0
42	35.235532164087786	38.0	34.0	40.0	26.0	41.0
43	35.164169746324696	38.0	34.0	40.0	26.0	41.0
44	35.024262295888136	38.0	34.0	40.0	25.0	41.0
45	34.88672267833125	38.0	34.0	40.0	25.0	41.0
46	34.81448574423635	37.0	34.0	40.0	24.0	41.0
47	34.737527077631974	37.0	34.0	40.0	24.0	41.0
48	34.580571066541104	37.0	34.0	40.0	24.0	41.0
49	34.426220982982585	37.0	33.0	40.0	24.0	41.0
50	34.32580282156506	37.0	33.0	40.0	24.0	41.0
51	34.043994646353624	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	2.0
12	3.0
13	6.0
14	25.0
15	76.0
16	178.0
17	408.0
18	958.0
19	1876.0
20	3269.0
21	5280.0
22	8200.0
23	12431.0
24	18383.0
25	26383.0
26	34835.0
27	40326.0
28	44662.0
29	51447.0
30	60080.0
31	72905.0
32	89167.0
33	113722.0
34	176356.0
35	234393.0
36	187608.0
37	247352.0
38	383007.0
39	626764.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.36771567646338	24.242010715488775	29.338547647206838	13.05172596084101
2	31.580571476342573	25.859456127884282	28.85543269707557	13.704539698697568
3	29.14372802951882	26.09427236880821	31.067827880105202	13.694171721567768
4	26.03915407141856	27.970220546953822	30.648641958916585	15.341983422711033
5	23.61394898627411	32.47266829111968	29.191305979904975	14.722076742701232
6	22.23152471553631	41.10759501451927	26.127343347241993	10.533536922702428
7	88.8333198098849	3.1900175640909003	6.3981483530488825	1.5785142729753145
8	88.75238402003765	2.659570544454034	6.42281840139726	2.1652270341110547
9	82.65453818243213	6.338973021130183	8.023175092594641	2.983313703843036
10	33.92988132969102	42.750325177464525	12.807238405282176	10.51255508756228
11	33.29780354609406	23.685787183541063	26.558864292604806	16.457544977760076
12	31.30047217325095	22.016051103882216	28.350434348575487	18.33304237429135
13	25.4758409740817	27.3861305152106	29.85018477948173	17.287843731225973
14	19.86807671155632	30.320718824558256	30.708063171715832	19.103141292169596
15	18.309847611226264	26.798885012167005	36.98302520360986	17.908242172996868
16	22.903066380461322	25.640990965516846	34.14719904794923	17.308743606072603
17	22.05494126315565	24.789915277644592	28.894035995321705	24.261107463878048
18	23.1712814409931	25.002397338585347	32.06770248085613	19.75861873956543
19	24.665089750619416	26.599393657748564	28.21745377234545	20.518062819286566
20	26.218892995099598	27.17352551382957	27.83945289864872	18.768128592422116
21	24.604644034151217	26.6673387410735	30.564960499236538	18.163056725538745
22	24.350403203663955	23.15066842717377	30.021932574544934	22.47699579461734
23	20.764681342476823	27.790276722538998	29.73015392962725	21.714888005356926
24	20.35615025944531	26.3555208043911	33.24678326338022	20.041545672783364
25	20.920651781038156	27.526610458297373	30.797563812235524	20.755173948428947
26	20.998227198851243	29.65684044707701	27.65467341691644	21.690258937155306
27	20.67284483359192	28.996568322510473	29.697533732807802	20.6330531110898
28	18.583349110689834	28.479685731450544	32.230147782605236	20.706817375254385
29	20.35852710795728	26.48702609533785	31.268425698486112	21.88602109821876
30	22.3116409024484	26.993294828387437	30.987670713046356	19.707393556117804
31	25.36076872198495	26.624063706096944	27.915594011325272	20.099573560592834
32	25.30351945696388	27.37014825797494	28.15749981763835	19.168832467422835
33	24.21422617598678	27.528741425928793	27.68590028874611	20.57113210933831
34	20.742347162493658	27.894899037212433	30.28576275937359	21.076991040920316
35	20.222473020720383	28.570579696960014	29.9281699987624	21.278777283557208
36	24.955229189666774	28.316420826766265	27.88395733802802	18.844392645538942
37	21.655097971236856	29.518942253236	28.637787137643294	20.18817263788385
38	20.493310810644676	30.567132447014718	27.90379172905894	21.035765013281665
39	20.72025886339104	28.321297464230476	28.848998814034555	22.109444858343927
40	23.270658296881493	26.724055264186713	28.64094260894367	21.364343829988126
41	19.894304005481505	26.376174798357187	29.290436954912824	24.439084241248484
42	21.99195477758845	26.307369131950338	28.457802333081716	23.2428737573795
43	21.72685420821029	26.383141423306068	28.984274278483046	22.9057300900006
44	21.42446170528226	27.844534436846725	28.761547180852766	21.96945667701825
45	20.861394488825944	29.00296122540474	27.13807768688381	22.997566598885506
46	22.29828137460526	28.190406875485102	27.997718225428507	21.51359352448113
47	20.914955540638783	27.80576721801356	29.451611872112437	21.82766536923522
48	21.24845197495621	27.622053220096994	29.98423083952748	21.145263965419314
49	22.449948897756993	25.467399063849527	30.138971873686078	21.943680164707406
50	20.521423191320736	28.309618122404416	29.379077012350596	21.78988167392425
51	20.15325755284595	27.740035062613565	28.31236379223721	23.79434359230327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1849.0
1	3031.0
2	4213.0
3	13821.5
4	23430.0
5	17040.5
6	10651.0
7	10009.5
8	9368.0
9	9879.5
10	10391.0
11	10730.5
12	11070.0
13	11307.0
14	11544.0
15	11014.5
16	10485.0
17	10033.5
18	9582.0
19	9809.5
20	10037.0
21	11193.0
22	12349.0
23	12529.5
24	12710.0
25	15272.0
26	22837.5
27	27841.0
28	33253.5
29	38666.0
30	43897.0
31	49128.0
32	56344.0
33	63560.0
34	70903.5
35	78247.0
36	85442.0
37	92637.0
38	98544.0
39	104451.0
40	123110.0
41	141769.0
42	159115.5
43	176462.0
44	188730.5
45	200999.0
46	205108.0
47	209217.0
48	203434.5
49	197652.0
50	185322.5
51	172993.0
52	162437.5
53	151882.0
54	143075.5
55	134269.0
56	125589.5
57	116910.0
58	106799.0
59	96688.0
60	85335.5
61	73983.0
62	64821.5
63	55660.0
64	47951.5
65	40243.0
66	32341.0
67	24439.0
68	19669.5
69	14900.0
70	11991.0
71	9082.0
72	7304.5
73	5527.0
74	4577.0
75	2748.0
76	1869.0
77	1422.0
78	975.0
79	780.5
80	586.0
81	403.0
82	220.0
83	157.0
84	94.0
85	68.0
86	42.0
87	44.5
88	47.0
89	29.5
90	12.0
91	9.0
92	6.0
93	6.0
94	6.0
95	4.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2440206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.89070415017695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18680337539485	17.43969576213866
2	8.831231986445294	4.0430623736659745
3	3.0565064502830257	2.0989675465960884
4	1.5085582069330667	1.38127838432905
5	0.9277056206781865	1.0617917450700325
6	0.6435031848496291	0.8838144614453693
7	0.485066689107825	0.7772462651431158
8	0.3738293301623736	0.6845773279524572
9	0.31157519157094554	0.64189579777067
>10	5.52315443953501	33.68506376654256
>50	1.9998269622294687	30.70055077775819
>100	0.1466001845539742	4.924816475463496
>500	0.0036376633912158407	0.5312957903205259
>1k	0.0018188316956079204	0.5577335951049106
>5k	0.0	0.0
>10k+	1.8188316956079204E-4	0.5882099306988786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14128	0.578967513398459	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09572962282692526	0.0
2	0.0	0.0	0.0	0.28296791336469135	0.0
3	0.0	0.0	0.0	0.4409053989704148	0.0
4	0.0	0.0	0.0	0.6642471988020684	0.0
5	0.0	0.0	0.0	1.125273849830711	0.0
6	0.0	0.0	0.0	1.629575535835909	0.0
7	0.0	0.0	0.0	1.9100846403951142	0.0
8	0.0	0.0	0.0	2.5803559207706233	0.0
9	0.0	0.0	0.0	2.8138198168515283	0.0
10	0.0	0.0	0.0	3.242554112234787	0.0
11	0.0	0.0	0.0	3.7713619260013296	0.0
12	0.0	0.0	0.0	4.230257609398551	0.0
13	0.0	0.0	0.0	4.440772623294919	0.0
14	0.0	0.0	0.0	4.53183050939142	0.0
15	0.0	0.0	0.0	4.674769261283679	0.0
16	0.0	0.0	0.0	4.956466790098869	0.0
17	0.0	0.0	0.0	5.305166858863555	0.0
18	0.0	0.0	0.0	5.730171960891826	0.0
19	0.0	0.0	0.0	5.959087060682582	0.0
20	0.0	0.0	0.0	6.220868238173335	0.0
21	0.0	0.0	0.0	6.544857278442885	0.0
22	0.0	0.0	0.0	6.891549320016425	0.0
23	0.0	0.0	0.0	7.268812551071508	0.0
24	0.0	0.0	0.0	7.549321655630713	0.0
25	0.0	0.0	0.0	7.7956123376469035	0.0
26	0.0	0.0	0.0	8.044197907881548	0.0
27	0.0	0.0	0.0	8.305610264051477	0.0
28	0.0	0.0	0.0	8.57648903412253	0.0
29	0.0	0.0	0.0	8.874701562081235	0.0
30	0.0	0.0	0.0	9.23766272191774	0.0
31	0.0	0.0	0.0	9.549480658600135	0.0
32	0.0	0.0	0.0	9.833268174899988	0.0
33	0.0	0.0	0.0	10.13545577709423	0.0
34	0.0	0.0	0.0	10.449281740967772	0.0
35	0.0	0.0	0.0	10.812406821391308	0.0
36	0.0	0.0	0.0	11.119184200022458	0.0
37	4.098014675810157E-5	0.0	0.0	11.436329555783406	0.0
38	4.098014675810157E-5	0.0	0.0	11.797528569309312	0.0
39	4.098014675810157E-5	0.0	0.0	12.276627465058278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	520	0.0	41.538464	31
CGTTTTT	7290	0.0	40.956787	1
CGACGGT	530	0.0	40.75472	28
TACGTAG	125	0.0	39.6	1
CACGACG	540	0.0	39.583332	26
GGGCGAT	3885	0.0	39.49807	7
GTAATCG	40	3.4603545E-7	39.375	1
AGGGCGA	1975	0.0	39.3038	6
ACACGAC	530	0.0	38.632076	26
TACGGGA	1330	0.0	38.57143	4
CGTTAGG	350	0.0	38.571426	2
GGCGATA	830	0.0	38.493973	8
TGGGCGA	1155	0.0	38.18182	6
AAGGGAT	4550	0.0	37.879124	5
TATGGGA	3190	0.0	37.876175	4
ATAGGGA	4200	0.0	37.875	4
TAAGGGA	4235	0.0	37.774498	4
AGGGATT	4910	0.0	37.53055	6
CACGACC	540	0.0	37.5	27
GCGATAT	180	0.0	37.5	9
>>END_MODULE