FastQCFastQC Report
Thu 26 May 2016
SRR1547955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547955_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2408841
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433991.8016548207208363No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.1510685014079385No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31800.13201369455269152No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT26470.10988687090596683No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA253.892082E-545.00000428
ATATCCG253.892082E-545.0000041
GCCCGAC253.892082E-545.0000049
CGGTACG207.0348283E-445.029
CGCTAGG1200.044.9999962
GTCCGTT302.1664982E-644.9999969
CGACGGT6800.044.33823428
CGTTTTT167350.043.7765161
CGGTCTA6750.043.66666831
GACGGTC6900.043.0434829
CTACGCG900.042.5000041
CACGACG7050.042.4468126
TATAGCG800.042.18751
ACGGTCT7050.042.12766330
GCGCGTC750.042.038
TCTCACG7350.041.32652723
GTACGTC1150.041.0869612
GGCGATA6850.040.7299278
GTTAGCG1450.040.344831
TACGCGG1350.040.0000042