##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547955_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2408841
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36212394259314	31.0	31.0	33.0	30.0	34.0
2	31.71886770442715	31.0	31.0	34.0	30.0	34.0
3	31.608744205200757	31.0	31.0	34.0	30.0	34.0
4	35.52088120386526	37.0	35.0	37.0	33.0	37.0
5	35.51362252635189	37.0	35.0	37.0	33.0	37.0
6	35.60071171156585	37.0	35.0	37.0	33.0	37.0
7	35.953775280311156	37.0	35.0	37.0	35.0	37.0
8	35.98195688299892	37.0	35.0	37.0	35.0	37.0
9	37.76832883531956	39.0	38.0	39.0	35.0	39.0
10	37.24677303317238	39.0	37.0	39.0	34.0	39.0
11	36.972715509242825	39.0	37.0	39.0	33.0	39.0
12	36.50599894306017	39.0	35.0	39.0	32.0	39.0
13	36.34852777746642	39.0	35.0	39.0	32.0	39.0
14	37.42262108624023	40.0	35.0	41.0	32.0	41.0
15	37.61210100625156	40.0	35.0	41.0	33.0	41.0
16	37.69035897346483	40.0	35.0	41.0	33.0	41.0
17	37.62926652278004	40.0	35.0	41.0	33.0	41.0
18	37.48352838564272	39.0	36.0	41.0	33.0	41.0
19	37.3519115624485	39.0	36.0	41.0	32.0	41.0
20	37.134044131596895	39.0	35.0	41.0	32.0	41.0
21	37.01718378257428	39.0	35.0	41.0	32.0	41.0
22	36.93551629185986	38.0	35.0	41.0	32.0	41.0
23	36.82069551290434	38.0	35.0	41.0	32.0	41.0
24	36.77060378829487	38.0	35.0	41.0	32.0	41.0
25	36.770474680562145	38.0	35.0	41.0	32.0	41.0
26	36.667689150093345	38.0	35.0	41.0	31.0	41.0
27	36.58978155884925	38.0	35.0	40.0	31.0	41.0
28	36.53107282713969	38.0	35.0	40.0	31.0	41.0
29	36.50887459985943	38.0	35.0	40.0	31.0	41.0
30	36.3972732114739	38.0	35.0	40.0	31.0	41.0
31	36.20466481598412	38.0	35.0	40.0	30.0	41.0
32	35.94982275708525	38.0	35.0	40.0	30.0	41.0
33	35.720081981334594	38.0	35.0	40.0	29.0	41.0
34	35.466626481365935	38.0	35.0	40.0	27.0	41.0
35	35.27532618383696	38.0	34.0	40.0	26.0	41.0
36	35.12926590007394	38.0	34.0	40.0	25.0	41.0
37	35.00943939429792	38.0	34.0	40.0	24.0	41.0
38	34.8799210906822	38.0	34.0	40.0	24.0	41.0
39	34.80628982983933	38.0	34.0	40.0	24.0	41.0
40	34.65373223056233	38.0	34.0	40.0	23.0	41.0
41	34.544707184907594	37.0	34.0	40.0	23.0	41.0
42	34.53927594224775	37.0	34.0	40.0	23.0	41.0
43	34.47500852069522	37.0	34.0	40.0	23.0	41.0
44	34.34733840880324	37.0	34.0	40.0	23.0	41.0
45	34.238326232408035	37.0	33.0	40.0	23.0	41.0
46	34.187299618364186	37.0	33.0	40.0	23.0	41.0
47	34.12651644504556	37.0	33.0	40.0	23.0	41.0
48	33.97995550557301	36.0	33.0	40.0	22.0	41.0
49	33.85639732967016	36.0	33.0	40.0	21.0	41.0
50	33.75256233184341	36.0	33.0	40.0	20.0	41.0
51	33.46905378976861	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	0.0
13	7.0
14	26.0
15	56.0
16	167.0
17	372.0
18	870.0
19	1825.0
20	3416.0
21	5557.0
22	8974.0
23	13963.0
24	21852.0
25	33826.0
26	44885.0
27	49097.0
28	49818.0
29	52512.0
30	58806.0
31	70005.0
32	86279.0
33	110818.0
34	181151.0
35	268137.0
36	188690.0
37	231111.0
38	337703.0
39	588807.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.838440561249165	24.234227165678433	28.617413934751195	16.309918338321207
2	33.24403727767835	25.545148060830915	29.138785000753476	12.07202966073726
3	27.0231202474551	25.189541360347157	35.03946503733538	12.74787335486236
4	23.741957231714338	27.173773611458792	35.061550347241685	14.022718809585191
5	21.906800822470228	31.595236049203745	33.46924931948601	13.02871380884002
6	19.753981271491146	40.29145136603039	29.938837806231295	10.015729556247175
7	85.61353779680768	2.9484718999718122	10.099213688242603	1.3387766149779083
8	86.15201252386521	2.425938449237621	9.728620527465283	1.6934284994318842
9	81.41703831842783	5.5062579888004235	11.068808609617655	2.007895083154098
10	37.32317741187567	38.50075617278185	15.58566962286012	8.59039679248236
11	29.68406798124077	22.902881510236668	31.385757715017306	16.027292793505257
12	29.089051539723876	22.964986065913028	31.801476311637007	16.144486082726093
13	21.77366625692605	30.718175255236858	32.21939513649925	15.288763351337842
14	16.310624071908443	34.76123994900452	32.45814065768558	16.469995321401456
15	14.995344234011293	28.641948555342587	41.327219189643486	15.035488021002633
16	18.605586670104003	26.443546917376448	39.553295547526794	15.39757086499275
17	18.449495006104595	26.158721144317955	31.875370769594173	23.516413079983277
18	19.675437274606335	27.776594636175655	35.09804092507559	17.449927164142423
19	21.834483886649224	29.37068075476962	30.687828710985904	18.107006647595256
20	23.01621402159794	29.22322394877869	30.14217210683478	17.618389922788595
21	21.044851030018172	28.63144557901497	33.39805325465649	16.925650136310367
22	20.298433977169932	26.09653356116074	32.495586051549274	21.109446410120054
23	16.825602021885214	30.559011574445964	32.11573532665709	20.499651077011723
24	17.345976758117285	29.34797273875694	36.508885393431946	16.797165109693832
25	17.753724716575316	30.297475009766107	33.720324421578674	18.2284758520799
26	16.965254244676174	33.66220518498315	30.419234810433732	18.953305759906943
27	16.76138026544716	33.004378454202666	33.21132445022316	17.02291683012702
28	15.60688314421749	30.91038387340634	36.0521927350124	17.430540247363773
29	16.288331193300014	28.615794898874604	35.59641337888221	19.49946052894317
30	18.233374473450095	30.463903595131438	33.89891653288864	17.40380539852983
31	22.534447063961466	29.573890514151824	29.829241531508305	18.0624208903784
32	22.50327024490201	29.794577558253117	30.49520495541217	17.206947241432704
33	21.238595656583396	30.025726064941605	29.402521793675877	19.333156484799122
34	17.553711515205862	30.79451902387912	30.797424985708894	20.854344475206126
35	17.691163509754276	30.751095651394177	30.59193197060329	20.965808868248256
36	22.91188999190897	29.84439404676357	28.77504160714634	18.46867435418112
37	18.18816601012686	33.34126245775458	29.987201313826855	18.4833702182917
38	18.699200154763222	33.25545355629533	27.676754090452626	20.368592198488816
39	17.97520052174469	32.622825665953044	29.386663544833386	20.015310267468877
40	20.194857194808623	30.8119963085982	28.934869507784033	20.05827698880914
41	17.453829455742408	29.74364019875118	30.139183117524155	22.663347227982253
42	19.50236649077295	30.39046578831895	28.67980908661053	21.427358634297573
43	19.541140324330247	30.290874325038473	29.227333809080797	20.94065154155048
44	18.49271080988741	32.67504995140817	29.10067538704298	19.731563851661445
45	17.36926596649592	34.65446660863046	26.961679911625545	21.014587513248074
46	20.39092659083767	32.73686391090155	27.85796156740939	19.014247930851393
47	19.24502281387605	31.7869049887477	28.827058323899337	20.14101387347691
48	19.81745578060154	30.875221735266045	29.581072391245417	19.726250092886993
49	19.619684321215058	29.35843420134413	29.75530555981071	21.266575917630096
50	18.57042453196371	31.932784272602465	29.426433708160893	20.070357487272926
51	17.486957420601858	32.52414750496193	28.44625278297737	21.54264229145884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1955.0
1	2906.0
2	3857.0
3	30751.0
4	57645.0
5	39672.5
6	21700.0
7	20476.5
8	19253.0
9	21607.0
10	23961.0
11	26084.5
12	28208.0
13	29370.0
14	30532.0
15	28910.0
16	27288.0
17	26147.5
18	25007.0
19	23533.0
20	22059.0
21	21831.0
22	21603.0
23	21614.5
24	21626.0
25	24721.5
26	31140.5
27	34464.0
28	39209.5
29	43955.0
30	52714.0
31	61473.0
32	67245.5
33	73018.0
34	81689.0
35	90360.0
36	93546.5
37	96733.0
38	107412.0
39	118091.0
40	133521.5
41	148952.0
42	164516.5
43	180081.0
44	183993.0
45	187905.0
46	186489.5
47	185074.0
48	189046.0
49	193018.0
50	185367.0
51	177716.0
52	160564.0
53	143412.0
54	123274.0
55	103136.0
56	90866.5
57	78597.0
58	67502.5
59	56408.0
60	47072.0
61	37736.0
62	31520.5
63	25305.0
64	22103.0
65	18901.0
66	14350.5
67	9800.0
68	7349.5
69	4899.0
70	4007.0
71	3115.0
72	2490.0
73	1865.0
74	1468.5
75	799.0
76	526.0
77	415.0
78	304.0
79	237.5
80	171.0
81	141.0
82	111.0
83	84.5
84	58.0
85	39.5
86	21.0
87	22.0
88	23.0
89	22.5
90	22.0
91	14.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2408841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.204849349327777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95019919838394	18.14158280298991
2	8.818811715842813	4.269160180441242
3	3.568267660261384	2.5910814346411533
4	2.1248778170994873	2.0572938979448625
5	1.483013165489388	1.794805512687017
6	1.025333000312712	1.4890818483278063
7	0.7198568058945944	1.2196817877836863
8	0.5676921702467015	1.0992722766091478
9	0.4594542913335586	1.0008919714167848
>10	4.554401121301564	25.70853024972611
>50	1.2598182277058996	21.316416708174053
>100	0.46077555201494164	15.639653865784478
>500	0.004534444812522531	0.6919243379096267
>1k	0.0027904275769369425	1.1485918681095133
>5k	0.0	0.0
>10k+	1.744017235585589E-4	1.832031257454694
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43399	1.8016548207208363	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3639	0.1510685014079385	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3180	0.13201369455269152	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	2647	0.10988687090596683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.302748085074939E-5	0.0	0.0	0.06351602285082328	0.0
2	8.302748085074939E-5	0.0	0.0	0.20159072350561952	0.0
3	8.302748085074939E-5	0.0	0.0	0.3325665745476767	0.0
4	8.302748085074939E-5	0.0	0.0	0.49401351106195884	0.0
5	8.302748085074939E-5	0.0	0.0	0.9349724618602888	0.0
6	8.302748085074939E-5	0.0	0.0	1.539703118636722	0.0
7	8.302748085074939E-5	0.0	0.0	1.9407258511458414	0.0
8	8.302748085074939E-5	0.0	0.0	2.6951135421557506	0.0
9	8.302748085074939E-5	0.0	0.0	3.0724734426224063	0.0
10	8.302748085074939E-5	0.0	0.0	3.575453921616246	0.0
11	8.302748085074939E-5	0.0	0.0	4.1126417227205945	0.0
12	8.302748085074939E-5	0.0	0.0	4.517068581944595	0.0
13	8.302748085074939E-5	0.0	0.0	4.694290739820519	0.0
14	8.302748085074939E-5	0.0	0.0	4.781428080973381	0.0
15	8.302748085074939E-5	0.0	0.0	4.88762022898149	0.0
16	8.302748085074939E-5	0.0	0.0	5.077379536465878	0.0
17	8.302748085074939E-5	0.0	0.0	5.343067475188275	0.0
18	8.302748085074939E-5	0.0	0.0	5.632584300914838	0.0
19	8.302748085074939E-5	0.0	0.0	5.812421824437561	0.0
20	8.302748085074939E-5	0.0	0.0	5.9980297578794115	0.0
21	8.302748085074939E-5	8.302748085074939E-5	0.0	6.225815651593443	0.0
22	8.302748085074939E-5	8.302748085074939E-5	0.0	6.5017159704604826	0.0
23	8.302748085074939E-5	8.302748085074939E-5	0.0	6.788451375578546	0.0
24	8.302748085074939E-5	8.302748085074939E-5	0.0	7.007602411284099	0.0
25	8.302748085074939E-5	8.302748085074939E-5	0.0	7.195659655411046	0.0
26	8.302748085074939E-5	8.302748085074939E-5	0.0	7.383675385797568	0.0
27	8.302748085074939E-5	8.302748085074939E-5	0.0	7.589168400903173	0.0
28	8.302748085074939E-5	8.302748085074939E-5	0.0	7.795035039672606	0.0
29	8.302748085074939E-5	8.302748085074939E-5	0.0	8.023941804378122	0.0
30	8.302748085074939E-5	8.302748085074939E-5	0.0	8.30237446141111	0.0
31	8.302748085074939E-5	8.302748085074939E-5	0.0	8.54938121694209	0.0
32	8.302748085074939E-5	8.302748085074939E-5	0.0	8.770940049592314	0.0
33	8.302748085074939E-5	8.302748085074939E-5	0.0	9.005700251697808	0.0
34	8.302748085074939E-5	8.302748085074939E-5	0.0	9.25457512554793	0.0
35	8.302748085074939E-5	8.302748085074939E-5	0.0	9.531347232963903	0.0
36	8.302748085074939E-5	8.302748085074939E-5	0.0	9.786283112916129	0.0
37	8.302748085074939E-5	8.302748085074939E-5	0.0	10.047529081413012	0.0
38	8.302748085074939E-5	8.302748085074939E-5	0.0	10.33206425828853	0.0
39	8.302748085074939E-5	8.302748085074939E-5	0.0	10.704317968682865	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCTA	25	3.892082E-5	45.000004	28
ATATCCG	25	3.892082E-5	45.000004	1
GCCCGAC	25	3.892082E-5	45.000004	9
CGGTACG	20	7.0348283E-4	45.0	29
CGCTAGG	120	0.0	44.999996	2
GTCCGTT	30	2.1664982E-6	44.999996	9
CGACGGT	680	0.0	44.338234	28
CGTTTTT	16735	0.0	43.776516	1
CGGTCTA	675	0.0	43.666668	31
GACGGTC	690	0.0	43.04348	29
CTACGCG	90	0.0	42.500004	1
CACGACG	705	0.0	42.44681	26
TATAGCG	80	0.0	42.1875	1
ACGGTCT	705	0.0	42.127663	30
GCGCGTC	75	0.0	42.0	38
TCTCACG	735	0.0	41.326527	23
GTACGTC	115	0.0	41.08696	12
GGCGATA	685	0.0	40.729927	8
GTTAGCG	145	0.0	40.34483	1
TACGCGG	135	0.0	40.000004	2
>>END_MODULE