FastQCFastQC Report
Thu 26 May 2016
SRR1547954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547954_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1893409
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.3910407101688013No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32820.17333814300027095No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG28090.14835674701028673No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC26600.14048734319948833No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG22420.1184107606967116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT406.8193913E-945.00000413
CGACTCG650.045.00000435
TACGTAA207.034444E-445.00000421
CTTAACG253.8917668E-545.01
TGCGACC253.8917668E-545.035
TAGTCCG253.8917668E-545.01
ATCGATC253.8917668E-545.040
CTATACG302.166249E-644.9999961
CGTACTC302.166249E-644.99999645
CGGTCTA4800.043.12499631
CGACGGT5050.041.8811928
TAAGACG700.041.7857171
GCGATAC603.6379788E-1241.2499969
TCACGAC5650.039.8230125
ACGACGG5500.039.68181627
CGTTTTT40050.039.3258441
ACGGGAC5550.039.324335
TAAGGGA26050.039.2130554
CGCATCG1150.039.13043621
AGCGGGA15300.038.382354
Produced by FastQC (version 0.11.5)