FastQCFastQC Report
Thu 26 May 2016
SRR1547953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547953_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1957291
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94980.4852625388866551No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32590.16650564479170443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2125201E-745.0000041
CCGTATC351.2125201E-745.00000422
CGGTACG253.891815E-545.06
TACCGGA207.034504E-445.03
TCTAGCG850.042.352941
CGGTCTA4150.041.74698631
ACGGTCT4150.041.74698630
CACGACG4150.041.2048226
CGACGGT4350.040.86207228
CGTTTTT50750.040.5221671
TCACGAC4300.040.290725
TAAGGGA27200.039.78864
TAACGCG800.039.3751
TATTACG403.4598816E-739.3751
TACGGGA11200.039.174114
GCGATAT2250.039.09
TCGATAG1100.038.8636361
ATGGGAC16400.038.6890265
TTAGGGA26050.038.6084444
CGTAGCA356.2505387E-638.5714310
Produced by FastQC (version 0.11.5)