##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547953_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1957291
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.378510400344148	31.0	31.0	33.0	30.0	34.0
2	31.781905194475424	31.0	31.0	34.0	30.0	34.0
3	31.897628916701706	31.0	31.0	34.0	30.0	34.0
4	35.66654524033473	37.0	35.0	37.0	33.0	37.0
5	35.580554960912814	37.0	35.0	37.0	33.0	37.0
6	35.63397113663732	37.0	35.0	37.0	33.0	37.0
7	35.93146701231447	37.0	35.0	37.0	35.0	37.0
8	35.93632423589543	37.0	35.0	37.0	35.0	37.0
9	37.703626083193555	39.0	37.0	39.0	35.0	39.0
10	37.17064197403452	39.0	37.0	39.0	33.0	39.0
11	36.951417545985755	39.0	37.0	39.0	33.0	39.0
12	36.32303934366428	38.0	35.0	39.0	32.0	39.0
13	36.091223022023804	38.0	35.0	39.0	32.0	39.0
14	37.040748667418384	39.0	35.0	41.0	31.0	41.0
15	37.269378952848605	39.0	35.0	41.0	32.0	41.0
16	37.396193003493096	39.0	35.0	41.0	32.0	41.0
17	37.356941813966344	39.0	35.0	41.0	32.0	41.0
18	37.284660277904514	39.0	35.0	41.0	32.0	41.0
19	37.23015279792325	39.0	35.0	41.0	32.0	41.0
20	37.07416934937115	39.0	35.0	41.0	32.0	41.0
21	36.950632787868535	39.0	35.0	41.0	32.0	41.0
22	36.884498523724886	38.0	35.0	41.0	31.0	41.0
23	36.778908705961456	38.0	35.0	41.0	31.0	41.0
24	36.7287633775458	38.0	35.0	41.0	31.0	41.0
25	36.75708313173667	38.0	35.0	41.0	31.0	41.0
26	36.652984150031855	38.0	35.0	41.0	31.0	41.0
27	36.5921424049873	38.0	35.0	41.0	31.0	41.0
28	36.54988808511356	38.0	35.0	40.0	31.0	41.0
29	36.54371782223492	38.0	35.0	40.0	31.0	41.0
30	36.464953346232114	38.0	35.0	40.0	31.0	41.0
31	36.32072747486194	38.0	35.0	40.0	30.0	41.0
32	36.19990640124539	38.0	35.0	40.0	30.0	41.0
33	36.08984203166519	38.0	35.0	40.0	30.0	41.0
34	35.95607040547369	38.0	35.0	40.0	30.0	41.0
35	35.89081490693004	38.0	35.0	40.0	30.0	41.0
36	35.76853518459953	38.0	35.0	40.0	29.0	41.0
37	35.65428594930442	38.0	35.0	40.0	29.0	41.0
38	35.5372389695758	38.0	34.0	40.0	28.0	41.0
39	35.48487731257131	38.0	34.0	40.0	28.0	41.0
40	35.32252690070102	38.0	34.0	40.0	27.0	41.0
41	35.21457514493246	38.0	34.0	40.0	26.0	41.0
42	35.2044458386617	38.0	34.0	40.0	27.0	41.0
43	35.15380186185907	38.0	34.0	40.0	27.0	41.0
44	35.013396577207985	37.0	34.0	40.0	26.0	41.0
45	34.91904576274044	37.0	34.0	40.0	26.0	41.0
46	34.874303820944355	37.0	34.0	40.0	26.0	41.0
47	34.79472546494108	37.0	34.0	40.0	26.0	41.0
48	34.665706836643096	37.0	34.0	40.0	25.0	41.0
49	34.53528320520556	36.0	34.0	40.0	24.0	41.0
50	34.43426194674169	36.0	34.0	40.0	24.0	41.0
51	34.087366671588434	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	0.0
12	8.0
13	15.0
14	24.0
15	68.0
16	141.0
17	357.0
18	839.0
19	1508.0
20	2678.0
21	4138.0
22	6470.0
23	9466.0
24	13866.0
25	20211.0
26	26337.0
27	30878.0
28	34419.0
29	39408.0
30	47809.0
31	58349.0
32	72292.0
33	94787.0
34	161430.0
35	225161.0
36	143760.0
37	184158.0
38	286506.0
39	492142.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.25258788805548	24.842550239080445	29.854988348692146	13.049873524171929
2	31.276238433630972	26.161311731367487	29.70171527892378	12.860734556077762
3	29.0973595648271	26.340692314019737	30.98139213841989	13.580555982733278
4	26.330423018345257	27.74957837133058	30.693851859534426	15.22614675078974
5	23.06391844646504	32.792160184663395	29.34484448147976	14.799076887391807
6	22.43095175934493	41.3021364733195	25.99521481476183	10.271696952573736
7	89.30051790970275	3.4296382091370163	5.730113713290462	1.539730167869775
8	89.77157714412421	2.787475137830808	5.484927892684328	1.9560198253606644
9	84.42234700920814	5.879095137105315	6.9795446870189455	2.719013166667603
10	39.851866687171196	36.82038082226914	12.881630784589518	10.446121705970139
11	37.27131019352768	22.719258403579232	24.36673953949617	15.64269186339691
12	34.807598870070926	22.472028942042854	26.60207398899806	16.118298198888155
13	24.685445342567867	31.398141615120085	27.42714292356119	16.489270118750866
14	17.753108761037577	35.55184180584287	29.40334370310802	17.29170573001153
15	16.736703944380267	27.77405097147026	39.58435408940214	15.904890994747332
16	21.153573995895346	24.527063170473884	37.01457780166567	17.304785031965096
17	21.055530322266847	24.503867845915607	27.780181894261	26.66041993755655
18	22.3317840832048	26.72571426527788	31.42894950214352	19.5135521493738
19	25.894258952807732	27.656592709004435	26.86943331369735	19.579715024490483
20	26.49176846978809	28.256963323287138	26.263698141972757	18.987570064952017
21	23.473208633769836	27.521405861468732	30.219829345764122	18.78555615899731
22	23.00383540311584	24.731784900661168	28.927584094547004	23.336795601675988
23	19.791793862026648	29.25982901878157	28.39041307603213	22.557964043159654
24	19.932702904167034	27.996858923890212	33.45608803187671	18.61435014006604
25	20.209411886122197	29.025985405338297	30.690275487906497	20.074327220633005
26	19.09154029727823	32.79757583312855	26.707270405882415	21.403613463710812
27	18.869038890997814	32.61140014438323	29.35250813496818	19.16705282965078
28	17.115594972847674	29.849572700226997	33.210238027968245	19.82459429895708
29	18.553551822391253	28.10072697416991	32.30817492135814	21.03754628208069
30	20.67490219900873	29.801189501203453	30.35741747139286	19.16649082839496
31	25.413901152153667	28.237242188310276	26.89947483537195	19.449381824164114
32	26.43730543899706	28.120754655286312	26.77430182839445	18.667638077322177
33	25.010792978662856	28.143030341425977	26.870966044395033	19.97521063551613
34	20.361867499518468	28.44426301454408	29.637034043481524	21.556835442455924
35	20.78888627189314	28.701250861522382	29.014541016128923	21.495321850455554
36	26.944179480721058	26.83939179202275	27.845731677098602	18.370697050157588
37	21.72466945385229	30.3181284745089	29.15258896096697	18.80461311067184
38	21.308992888640475	30.510792723207743	26.59016978057938	21.590044607572406
39	21.086695846453082	29.338662467665767	28.208835579379866	21.36580610650128
40	23.198900929907715	26.95730987369788	27.755556021051547	22.088233175342857
41	19.53373310355997	26.276419806763535	28.73430675356909	25.455540336107408
42	21.706021230363802	27.420960909747194	27.2712131205835	23.6018047393055
43	22.148418400738574	27.520230767933846	27.8753133795639	22.456037451763688
44	21.273280263384443	30.1641912214382	27.375796445188783	21.18673206998857
45	19.405341362117333	32.38046871926555	24.875044129871338	23.33914578874577
46	21.967556178411897	30.118822392786765	27.44660860342177	20.46701282537957
47	21.44555919380409	28.058372515890582	29.171083911385686	21.32498437891964
48	21.75813407408505	27.701808264586102	29.681687597807375	20.85837006352147
49	22.11526032664535	26.03424835652951	29.672031394411967	22.17845992241317
50	20.399215037518694	29.889525880413288	28.31372545012469	21.397533631943332
51	19.52698908849016	30.459957155067897	26.872141137929923	23.140912618512015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1363.0
1	2189.5
2	3016.0
3	8951.5
4	14887.0
5	10952.5
6	7018.0
7	6929.0
8	6840.0
9	7535.5
10	8231.0
11	8718.5
12	9206.0
13	9333.0
14	9460.0
15	8981.0
16	8502.0
17	8166.0
18	7830.0
19	7965.5
20	8101.0
21	8486.0
22	8871.0
23	9684.5
24	10498.0
25	13047.5
26	18952.0
27	22307.0
28	25072.5
29	27838.0
30	33781.5
31	39725.0
32	46485.5
33	53246.0
34	59445.5
35	65645.0
36	71905.5
37	78166.0
38	83792.0
39	89418.0
40	104293.5
41	119169.0
42	136824.0
43	154479.0
44	162263.5
45	170048.0
46	170934.5
47	171821.0
48	172145.0
49	172469.0
50	159617.5
51	146766.0
52	131920.0
53	117074.0
54	108361.5
55	99649.0
56	91673.0
57	83697.0
58	76836.0
59	69975.0
60	61299.5
61	52624.0
62	45774.5
63	38925.0
64	31806.5
65	24688.0
66	20506.5
67	16325.0
68	12879.0
69	9433.0
70	7659.0
71	5885.0
72	4802.0
73	3719.0
74	2846.5
75	1593.5
76	1213.0
77	955.5
78	698.0
79	574.5
80	451.0
81	323.0
82	195.0
83	157.0
84	119.0
85	92.0
86	65.0
87	46.5
88	28.0
89	21.0
90	14.0
91	13.0
92	12.0
93	11.0
94	10.0
95	5.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1957291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.080593614767086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92961358433958	16.21721920912528
2	8.757012991724702	3.6920606431556826
3	3.000940874443212	1.8978484510824338
4	1.5206234741636016	1.2822258199967265
5	0.9443635977313745	0.9953872614177242
6	0.6660469616661527	0.842439919634071
7	0.4998476849113647	0.7375960140429038
8	0.38540969060494734	0.6499732050268808
9	0.310338867305569	0.5887914790082244
>10	4.123251073416614	22.20523100620497
>50	2.3206515705175366	35.26025801999836
>100	0.537712223007475	14.348933347347096
>500	0.0032021341283787873	0.4652506760096565
>1k	7.38954029625874E-4	0.3235984612602879
>5k	2.463180098752913E-4	0.49318648668970066
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9498	0.4852625388866551	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3259	0.16650564479170443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07505271316324451	0.0
2	0.0	0.0	0.0	0.2439085450247306	0.0
3	0.0	0.0	0.0	0.3838979487465073	0.0
4	0.0	0.0	0.0	0.6129900970269623	0.0
5	0.0	0.0	0.0	1.0805751418669989	0.0
6	0.0	0.0	0.0	1.5724795137769498	0.0
7	0.0	0.0	0.0	1.8491374047088553	0.0
8	0.0	0.0	0.0	2.4138464847587815	0.0
9	0.0	0.0	0.0	2.6459019123880916	0.0
10	0.0	0.0	0.0	3.058666289274308	0.0
11	0.0	0.0	0.0	3.5919032989984627	0.0
12	0.0	0.0	0.0	4.016674066349868	0.0
13	0.0	0.0	0.0	4.195237192630017	0.0
14	0.0	0.0	0.0	4.2732020941188615	0.0
15	0.0	0.0	0.0	4.389689627142821	0.0
16	0.0	0.0	0.0	4.650049481656024	0.0
17	0.0	0.0	0.0	4.94974942407644	0.0
18	0.0	0.0	0.0	5.303759124218116	0.0
19	0.0	0.0	0.0	5.511546315800768	0.0
20	0.0	0.0	0.0	5.730420259429998	0.0
21	0.0	0.0	0.0	6.008355425943306	0.0
22	0.0	0.0	0.0	6.300238441805536	0.0
23	0.0	0.0	0.0	6.607346582598091	0.0
24	0.0	0.0	0.0	6.83981073841345	0.0
25	0.0	0.0	0.0	7.039372275251866	0.0
26	0.0	0.0	0.0	7.232802889299547	0.0
27	0.0	0.0	0.0	7.451523559859009	0.0
28	0.0	0.0	0.0	7.666259130604494	0.0
29	0.0	0.0	0.0	7.912415680652494	0.0
30	0.0	0.0	0.0	8.218450909956669	0.0
31	0.0	0.0	0.0	8.480496768237323	0.0
32	0.0	0.0	0.0	8.714084926564318	0.0
33	0.0	0.0	0.0	8.953599643589023	0.0
34	0.0	0.0	0.0	9.209514579078942	0.0
35	5.109102325612288E-4	0.0	0.0	9.518053268522667	0.0
36	5.109102325612288E-4	0.0	0.0	9.780661128059139	0.0
37	5.109102325612288E-4	0.0	0.0	10.0573701100143	0.0
38	5.109102325612288E-4	0.0	0.0	10.371477720992944	0.0
39	5.109102325612288E-4	0.0	0.0	10.872118657879692	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2125201E-7	45.000004	1
CCGTATC	35	1.2125201E-7	45.000004	22
CGGTACG	25	3.891815E-5	45.0	6
TACCGGA	20	7.034504E-4	45.0	3
TCTAGCG	85	0.0	42.35294	1
CGGTCTA	415	0.0	41.746986	31
ACGGTCT	415	0.0	41.746986	30
CACGACG	415	0.0	41.20482	26
CGACGGT	435	0.0	40.862072	28
CGTTTTT	5075	0.0	40.522167	1
TCACGAC	430	0.0	40.2907	25
TAAGGGA	2720	0.0	39.7886	4
TAACGCG	80	0.0	39.375	1
TATTACG	40	3.4598816E-7	39.375	1
TACGGGA	1120	0.0	39.17411	4
GCGATAT	225	0.0	39.0	9
TCGATAG	110	0.0	38.863636	1
ATGGGAC	1640	0.0	38.689026	5
TTAGGGA	2605	0.0	38.608444	4
CGTAGCA	35	6.2505387E-6	38.57143	10
>>END_MODULE