FastQCFastQC Report
Thu 26 May 2016
SRR1547952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547952_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2021230
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84690.41900229068438527No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27060.1338788757340827No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG23050.11403947101517393No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC21850.10810249204692192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGC302.1663218E-645.00000436
CACGCTA207.034559E-445.021
CTACGCG253.8918595E-545.01
TTCGCGA207.034559E-445.02
CGATACG650.044.99999610
CGGTCTA5550.043.78378331
CGACGGT5600.043.3928628
GCGATAT1600.042.18759
CGTTTTT45100.041.7572061
CGTGCGG1350.041.6666642
TCGATAG1050.040.7142871
GACGGTC6100.040.57377229
TCGGTTA501.0822987E-940.519
GGCGATA6050.040.165298
TAGTACG850.039.7058831
ACGGTCT6150.039.512230
TACGATG800.039.3751
AGCGATA403.4599543E-739.37512
GACCGAT8050.039.1304369
TAAGGGA29100.039.123714
Produced by FastQC (version 0.11.5)