##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547952_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2021230
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31981664629953	31.0	31.0	33.0	30.0	34.0
2	31.714036997273936	31.0	31.0	34.0	30.0	34.0
3	31.84718463509843	31.0	31.0	34.0	30.0	34.0
4	35.63017024287191	37.0	35.0	37.0	33.0	37.0
5	35.52513716895158	37.0	35.0	37.0	33.0	37.0
6	35.577172810615316	37.0	35.0	37.0	33.0	37.0
7	35.861986513162776	37.0	35.0	37.0	35.0	37.0
8	35.85152011399	37.0	35.0	37.0	35.0	37.0
9	37.62020650791845	39.0	37.0	39.0	35.0	39.0
10	37.09361576861614	39.0	37.0	39.0	33.0	39.0
11	36.86445827540656	39.0	37.0	39.0	33.0	39.0
12	36.200216699732344	38.0	35.0	39.0	32.0	39.0
13	35.90878920261425	38.0	35.0	39.0	31.0	39.0
14	36.932294197097804	39.0	35.0	41.0	31.0	41.0
15	37.122121678383955	39.0	35.0	41.0	32.0	41.0
16	37.24181166913216	39.0	35.0	41.0	32.0	41.0
17	37.17584639056416	39.0	35.0	41.0	32.0	41.0
18	37.106897780064614	39.0	35.0	41.0	32.0	41.0
19	37.0467047292985	39.0	35.0	41.0	32.0	41.0
20	36.91285454896276	38.0	35.0	41.0	32.0	41.0
21	36.796721303364784	38.0	35.0	41.0	31.0	41.0
22	36.70006778050989	38.0	35.0	41.0	31.0	41.0
23	36.59525190107014	38.0	35.0	40.0	31.0	41.0
24	36.53115578138065	38.0	35.0	40.0	31.0	41.0
25	36.5786070857844	38.0	35.0	40.0	31.0	41.0
26	36.461094482072795	38.0	35.0	40.0	31.0	41.0
27	36.38239438361789	38.0	35.0	40.0	31.0	41.0
28	36.346621611592944	38.0	35.0	40.0	30.0	41.0
29	36.36164761061334	38.0	35.0	40.0	30.0	41.0
30	36.29487985038813	38.0	35.0	40.0	30.0	41.0
31	36.156356772856135	38.0	35.0	40.0	30.0	41.0
32	36.01504430470555	38.0	35.0	40.0	30.0	41.0
33	35.92390326682268	38.0	35.0	40.0	30.0	41.0
34	35.795980665238496	38.0	35.0	40.0	29.0	41.0
35	35.743403768992145	38.0	35.0	40.0	29.0	41.0
36	35.626039095006504	38.0	35.0	40.0	29.0	41.0
37	35.52239725315773	38.0	34.0	40.0	28.0	41.0
38	35.42787856899017	38.0	34.0	40.0	27.0	41.0
39	35.36555612176744	38.0	34.0	40.0	27.0	41.0
40	35.2132404525957	38.0	34.0	40.0	27.0	41.0
41	35.115523715757234	37.0	34.0	40.0	26.0	41.0
42	35.09548146425691	37.0	34.0	40.0	26.0	41.0
43	35.01775552510105	37.0	34.0	40.0	26.0	41.0
44	34.89715519757771	37.0	34.0	40.0	26.0	41.0
45	34.79022723787001	37.0	34.0	40.0	26.0	41.0
46	34.73055367276361	37.0	34.0	40.0	26.0	41.0
47	34.66330155400424	37.0	34.0	40.0	26.0	41.0
48	34.550333212944594	36.0	34.0	40.0	25.0	41.0
49	34.42114306635069	36.0	33.0	40.0	24.0	41.0
50	34.306107172365344	36.0	33.0	40.0	24.0	41.0
51	33.98318103333119	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	4.0
12	2.0
13	3.0
14	21.0
15	64.0
16	141.0
17	382.0
18	917.0
19	1740.0
20	3019.0
21	4720.0
22	7199.0
23	10539.0
24	15211.0
25	21497.0
26	28215.0
27	33082.0
28	37017.0
29	43075.0
30	51233.0
31	62580.0
32	77585.0
33	101424.0
34	172141.0
35	243265.0
36	146621.0
37	189348.0
38	292002.0
39	478095.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.95150477679433	24.505424914532238	28.78494777932249	12.75812252935094
2	30.09805910262563	26.512569079224036	30.245296181038277	13.144075637112055
3	28.870687650588998	26.86863939284495	30.548923180439637	13.711749776126418
4	26.677369720417765	28.232264512202963	30.33108552712952	14.759280240249751
5	24.056589304532388	32.56497281358381	28.83090989150171	14.547527990382095
6	21.648253786060963	41.747896083078125	25.810768690351914	10.793081440508997
7	89.35751992598566	3.4639798538513675	5.603221800586771	1.5752784195761986
8	89.55076859140226	3.164360315253583	5.419027028096753	1.865844065247399
9	84.40073618539206	5.938661112293009	6.783938492897888	2.8766642094170383
10	40.50261474448727	37.20516715069537	12.120293088861732	10.171925015955631
11	38.568248046981296	22.529449889423766	23.2464885243144	15.655813539280535
12	36.50420783384375	21.764024875941875	25.117626395808497	16.61414089440588
13	24.482270696556057	32.81467225402354	25.82477006575204	16.87828698366836
14	18.788361542229236	35.87746075409528	27.556982629389037	17.77719507428645
15	17.018201788020164	26.97990827367494	39.475715282278614	16.52617465602628
16	20.951153505538706	24.259436085947666	37.67923492131029	17.110175487203335
17	20.641045304097013	24.062575758325377	27.498800235500166	27.79757870207745
18	23.21432988823637	25.544544658450548	31.579434304853976	19.661691148459106
19	26.338368221330576	27.954611795787716	25.608614556482934	20.098405426398777
20	27.93600926168719	27.11640931512	25.804980135857868	19.14260128733494
21	24.27393220959515	27.45857720299026	28.63672120441513	19.63076938299946
22	23.42885272828921	24.264581467720152	28.054550941753288	24.25201486223735
23	19.309925144590174	29.91856443848548	27.90330640253707	22.868204014387278
24	20.724954606848307	26.303537944716833	33.82539344854371	19.146113999891156
25	20.047495831746016	27.70407128332748	30.949867160095586	21.29856572483092
26	19.537014590125814	33.155850645399084	25.93128936340743	21.375845401067668
27	19.321997001825622	31.801526793091334	28.733592911247115	20.14288329383593
28	17.200565991994974	29.6481350464816	33.908461679274495	19.242837282248928
29	18.69089613750043	26.761229548344325	32.87953374925169	21.66834056490355
30	19.929745748875686	28.332747881240632	30.690767502956124	21.046738866927566
31	26.086046615179864	27.386888181948617	26.088520356416637	20.43854484645488
32	25.844658945295684	28.232907684924523	26.792992385824473	19.129440983955316
33	24.676113059869486	27.57098400478916	26.053887979101837	21.69901495623952
34	20.30921765459646	28.10565843570499	28.57611454411423	23.009009365584323
35	19.905354660281116	28.33022466517912	28.910415934851553	22.85400473968821
36	25.494871934416174	27.13990985686933	26.35716865473004	21.008049553984456
37	20.773192560965352	31.510367449523308	27.28026993464376	20.43617005486758
38	20.9274055896657	31.231923135912293	24.93694433587469	22.90372693854732
39	20.699475072109557	29.971997249199745	27.057781647808515	22.270746030882187
40	23.426280037402964	26.930977672011597	25.886415697372396	23.756326593213043
41	18.87444773726889	26.200877683390804	27.57316089707752	27.35151368226278
42	22.08902499962894	27.062333331684172	25.753179994359872	25.09546167432702
43	21.616194099632402	27.133626554127932	26.494263394071925	24.75591595216774
44	20.13541259530088	30.10820144169639	27.40742023421382	22.348965728788905
45	19.096193901733102	32.41501461981071	24.63123939383445	23.85755208462174
46	22.095209352720868	30.254003750191714	26.61082608114861	21.03996081593881
47	21.10937399504262	28.482112377116902	27.699173275678675	22.709340352161803
48	21.6263859135279	26.776121470589693	29.0938191101458	22.503673505736607
49	21.081173344943426	25.952563538043666	29.357717825284602	23.6085452917283
50	19.74248353725207	30.095387462089917	28.019028017593246	22.143100983064766
51	19.457607496425446	30.591718903835783	26.15966515438619	23.79100844535258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1250.0
1	2066.0
2	2882.0
3	8515.0
4	14148.0
5	10526.0
6	6904.0
7	6911.5
8	6919.0
9	7535.0
10	8151.0
11	8656.0
12	9161.0
13	9084.0
14	9007.0
15	8684.0
16	8361.0
17	8032.5
18	7704.0
19	7334.0
20	6964.0
21	6972.5
22	6981.0
23	7978.0
24	8975.0
25	11096.5
26	15618.5
27	18019.0
28	21077.0
29	24135.0
30	28889.5
31	33644.0
32	39022.5
33	44401.0
34	51645.5
35	58890.0
36	65722.5
37	72555.0
38	82875.5
39	93196.0
40	110320.0
41	127444.0
42	146915.5
43	166387.0
44	169198.0
45	172009.0
46	169795.0
47	167581.0
48	169149.5
49	170718.0
50	167260.0
51	163802.0
52	151552.0
53	139302.0
54	125661.0
55	112020.0
56	101751.5
57	91483.0
58	83603.5
59	75724.0
60	67524.5
61	59325.0
62	52459.5
63	45594.0
64	37192.0
65	28790.0
66	23882.0
67	18974.0
68	14635.0
69	10296.0
70	8242.5
71	6189.0
72	5291.5
73	4394.0
74	3409.5
75	2024.0
76	1623.0
77	1279.0
78	935.0
79	620.5
80	306.0
81	286.5
82	267.0
83	179.0
84	91.0
85	72.5
86	54.0
87	36.0
88	18.0
89	11.0
90	4.0
91	5.5
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2021230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.320374129778283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5903753914152	15.766654568234987
2	8.712518165598816	3.5408325747491505
3	3.0275045347517597	1.8456007447726828
4	1.4762570858754123	1.199923851868984
5	0.8863630351413758	0.9005614244439284
6	0.6258689976200875	0.7630735312721695
7	0.4606005716175091	0.6551703157760264
8	0.35344070493874774	0.5745637885638343
9	0.2978601285870094	0.5447366326108707
>10	3.6220191257689076	18.24593352457951
>50	2.017700690550105	29.988986242109085
>100	0.9223245233831138	24.256349791950896
>500	0.005931347381030993	0.8535836592926197
>1k	9.88557896838499E-4	0.4387190002694785
>5k	2.4713947420962475E-4	0.42531034950574786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8469	0.41900229068438527	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2706	0.1338788757340827	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	2305	0.11403947101517393	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2185	0.10810249204692192	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.894964947086675E-5	0.0	0.0	0.05041484640540661	0.0
2	9.894964947086675E-5	0.0	0.0	0.18815275846885313	0.0
3	9.894964947086675E-5	0.0	0.0	0.2847770911771545	0.0
4	9.894964947086675E-5	0.0	0.0	0.4898007648807904	0.0
5	9.894964947086675E-5	0.0	0.0	0.953132498528124	0.0
6	9.894964947086675E-5	0.0	0.0	1.3420540957733658	0.0
7	9.894964947086675E-5	0.0	0.0	1.599521083696561	0.0
8	9.894964947086675E-5	0.0	0.0	2.1576960563617202	0.0
9	9.894964947086675E-5	0.0	0.0	2.3314516408325625	0.0
10	9.894964947086675E-5	0.0	0.0	2.7738555236168074	0.0
11	9.894964947086675E-5	0.0	0.0	3.1939462604453723	0.0
12	9.894964947086675E-5	0.0	0.0	3.617302335706476	0.0
13	1.4842447420630012E-4	0.0	0.0	3.7642920399954485	0.0
14	1.4842447420630012E-4	0.0	0.0	3.8260860960900045	0.0
15	1.4842447420630012E-4	0.0	0.0	3.9414613873730353	0.0
16	1.4842447420630012E-4	0.0	0.0	4.151234644251272	0.0
17	1.4842447420630012E-4	0.0	0.0	4.398460343454233	0.0
18	1.4842447420630012E-4	0.0	0.0	4.690213384919084	0.0
19	1.4842447420630012E-4	0.0	0.0	4.8667890343998454	0.0
20	1.4842447420630012E-4	0.0	0.0	5.053803871899784	0.0
21	1.4842447420630012E-4	0.0	0.0	5.295884189330259	0.0
22	1.4842447420630012E-4	0.0	0.0	5.571409488281888	0.0
23	1.4842447420630012E-4	0.0	0.0	5.861876184303617	0.0
24	1.4842447420630012E-4	0.0	0.0	6.0648713901931	0.0
25	1.4842447420630012E-4	0.0	0.0	6.248422989961558	0.0
26	1.4842447420630012E-4	0.0	0.0	6.427225006555414	0.0
27	1.4842447420630012E-4	0.0	0.0	6.614190369230617	0.0
28	1.4842447420630012E-4	0.0	0.0	6.803481048668385	0.0
29	1.4842447420630012E-4	0.0	0.0	7.017113341875986	0.0
30	1.4842447420630012E-4	0.0	0.0	7.289076453446664	0.0
31	1.4842447420630012E-4	0.0	0.0	7.524279770238914	0.0
32	1.4842447420630012E-4	0.0	0.0	7.743552193466354	0.0
33	1.4842447420630012E-4	0.0	0.0	7.970839538300935	0.0
34	1.4842447420630012E-4	0.0	0.0	8.20708182641263	0.0
35	1.4842447420630012E-4	0.0	0.0	8.49364001128026	0.0
36	1.4842447420630012E-4	0.0	0.0	8.741607832854251	0.0
37	1.4842447420630012E-4	0.0	0.0	8.990911474696102	0.0
38	1.4842447420630012E-4	0.0	0.0	9.264655679957254	0.0
39	1.4842447420630012E-4	0.0	0.0	9.649124542976306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCGC	30	2.1663218E-6	45.000004	36
CACGCTA	20	7.034559E-4	45.0	21
CTACGCG	25	3.8918595E-5	45.0	1
TTCGCGA	20	7.034559E-4	45.0	2
CGATACG	65	0.0	44.999996	10
CGGTCTA	555	0.0	43.783783	31
CGACGGT	560	0.0	43.39286	28
GCGATAT	160	0.0	42.1875	9
CGTTTTT	4510	0.0	41.757206	1
CGTGCGG	135	0.0	41.666664	2
TCGATAG	105	0.0	40.714287	1
GACGGTC	610	0.0	40.573772	29
TCGGTTA	50	1.0822987E-9	40.5	19
GGCGATA	605	0.0	40.16529	8
TAGTACG	85	0.0	39.705883	1
ACGGTCT	615	0.0	39.5122	30
TACGATG	80	0.0	39.375	1
AGCGATA	40	3.4599543E-7	39.375	12
GACCGAT	805	0.0	39.130436	9
TAAGGGA	2910	0.0	39.12371	4
>>END_MODULE