FastQCFastQC Report
Thu 26 May 2016
SRR1547951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547951_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3491465
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488031.3977800149793855No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA142650.40856775021373554No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT56720.16245329682525816No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.11917633428947447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG502.1827873E-1145.01
CCGCGTA207.0352654E-445.030
CCGCGAC253.892445E-545.042
CGTTTTT217650.043.4079971
CGACGGT3500.043.07142628
CGGTCTA3700.041.35135331
TCGCGCG501.0822987E-940.51
ACGGGAT17450.040.4871065
CGGTTTT16650.039.729731
CGAGGGT2450.039.4897964
CGAGACA12850.039.3968922
TTTCGCG403.4609184E-739.3751
TACCGCG403.4609184E-739.3751
GGGCGAT40450.039.2707067
TACGGGA16250.039.1846164
CACGACC12950.038.91892227
AGGGCGA20500.038.7439046
ACACGAC12950.038.39768626
GGCGATT11950.038.221768
GCGAGAC13100.038.12977221