FastQCFastQC Report
Thu 26 May 2016
SRR1547950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547950_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3185929
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311560.9779251201141018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149250.042.994981
CGACGGT4600.040.59782428
CGGTCTA4750.039.3157931
CGTTAGG5450.038.3944972
TACGGGA16200.038.3333324
TAGGGAC41850.038.2795685
ATAGCGG5300.038.2075422
TAAGGGA56550.037.997354
TATAGCG2150.037.674421
TATGGGA45400.037.6651954
TAGGGAT62750.037.577695
CGGGATC12400.037.5604826
TTAGGGA57100.037.5525364
AGGGCGA23250.037.354846
ACGGGAT20150.037.2952885
GGGCGAT41350.037.164457
TTACGGG10050.037.1641773
AGGGATC46450.037.1528556
GTAGGGA56800.037.1170774
AAGGGAT62700.037.1052635