##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547950_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3185929
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38379951342293	31.0	31.0	33.0	30.0	34.0
2	31.776682405665664	31.0	31.0	34.0	30.0	34.0
3	31.78831574714942	31.0	31.0	34.0	30.0	34.0
4	35.62501926439666	37.0	35.0	37.0	33.0	37.0
5	35.56050244685302	37.0	35.0	37.0	33.0	37.0
6	35.63450378209935	37.0	35.0	37.0	33.0	37.0
7	35.95746295664467	37.0	35.0	37.0	35.0	37.0
8	35.97335094410453	37.0	35.0	37.0	35.0	37.0
9	37.74030871372212	39.0	38.0	39.0	35.0	39.0
10	37.22274413522712	39.0	37.0	39.0	34.0	39.0
11	36.99431280483652	39.0	37.0	39.0	33.0	39.0
12	36.64991404390995	39.0	35.0	39.0	32.0	39.0
13	36.540011720286294	39.0	35.0	39.0	32.0	39.0
14	37.596204749070054	40.0	36.0	41.0	32.0	41.0
15	37.75593712226481	40.0	36.0	41.0	33.0	41.0
16	37.81692561259212	40.0	36.0	41.0	33.0	41.0
17	37.754753793948325	40.0	36.0	41.0	33.0	41.0
18	37.626959985611734	39.0	36.0	41.0	33.0	41.0
19	37.54650056545516	39.0	36.0	41.0	32.0	41.0
20	37.374968494275926	39.0	35.0	41.0	32.0	41.0
21	37.27749708169893	39.0	35.0	41.0	32.0	41.0
22	37.22302474411702	39.0	35.0	41.0	32.0	41.0
23	37.07331048494803	39.0	35.0	41.0	32.0	41.0
24	37.04672200792924	39.0	35.0	41.0	32.0	41.0
25	37.09154535458888	39.0	35.0	41.0	32.0	41.0
26	37.0095124530396	39.0	35.0	41.0	32.0	41.0
27	36.95712114111771	39.0	35.0	41.0	31.0	41.0
28	36.89067552980622	39.0	35.0	41.0	31.0	41.0
29	36.87365255157915	39.0	35.0	41.0	31.0	41.0
30	36.788899878183095	39.0	35.0	41.0	31.0	41.0
31	36.61790548376941	39.0	35.0	41.0	31.0	41.0
32	36.472259111863444	39.0	35.0	41.0	30.0	41.0
33	36.30303657112258	39.0	35.0	41.0	30.0	41.0
34	36.12553606812958	39.0	35.0	41.0	30.0	41.0
35	35.993023385015796	39.0	35.0	41.0	29.0	41.0
36	35.903806393676696	39.0	35.0	41.0	29.0	41.0
37	35.815532298428494	39.0	35.0	41.0	28.0	41.0
38	35.701332955003075	38.0	35.0	41.0	27.0	41.0
39	35.62239145944558	38.0	35.0	40.0	27.0	41.0
40	35.493942583152354	38.0	35.0	40.0	26.0	41.0
41	35.38271129080403	38.0	35.0	40.0	26.0	41.0
42	35.372106534703065	38.0	35.0	40.0	26.0	41.0
43	35.31230827805641	38.0	34.0	40.0	26.0	41.0
44	35.140600433970754	38.0	34.0	40.0	25.0	41.0
45	35.03668537497226	38.0	34.0	40.0	24.0	41.0
46	34.99736560356492	38.0	34.0	40.0	24.0	41.0
47	34.941977049708264	38.0	34.0	40.0	24.0	41.0
48	34.8182005939241	38.0	34.0	40.0	24.0	41.0
49	34.68322646235996	38.0	34.0	40.0	24.0	41.0
50	34.60379311654466	37.0	34.0	40.0	24.0	41.0
51	34.29574984251062	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	5.0
12	6.0
13	5.0
14	24.0
15	58.0
16	186.0
17	435.0
18	1017.0
19	2010.0
20	3618.0
21	5984.0
22	9519.0
23	14663.0
24	22520.0
25	34574.0
26	47275.0
27	54694.0
28	58198.0
29	63463.0
30	72692.0
31	88315.0
32	108243.0
33	139915.0
34	218076.0
35	288237.0
36	237825.0
37	316753.0
38	502324.0
39	895164.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.912920846635313	24.377316631977674	29.047288875552468	14.662473645834542
2	32.72276312497861	24.852782343862653	29.09198541461533	13.3324691165434
3	28.244665841580275	25.28590561811013	33.10849049052882	13.36093804978077
4	25.777065339497522	27.126844320761702	32.663125888869466	14.432964450871316
5	23.287461836092394	31.99355666745869	30.60689676386385	14.112084732585064
6	21.764515153978635	40.5068976741164	27.278448452554972	10.450138719349992
7	87.57169415890938	2.7772119215462743	7.912385994791473	1.7387079247528743
8	88.08174946773767	2.014859715957261	7.840036610985367	2.0633542053197043
9	82.45190021497653	5.322215278494906	9.507776224768348	2.7181082817602022
10	34.32261045365418	41.642861469919765	14.425556878386178	9.608971198039882
11	28.95707970893262	23.230429805560636	30.791301375517154	17.02118910998958
12	28.812663433491455	21.84885476104458	31.052041649390176	18.286440156073784
13	24.154963905347547	25.172218213274682	32.425549973022	18.247267908355774
14	18.073064402879034	29.73242655438963	32.93711190676252	19.25739713596882
15	17.537270918466795	27.137045426938265	37.80024601929296	17.52543763530198
16	22.124002135640815	25.98971916825516	34.85090220152426	17.03537649457976
17	21.828891980957515	25.19199266524772	30.68310687400755	22.296008479787215
18	21.970891378935313	25.93146300498222	33.20773940662205	18.88990620946041
19	23.319948435762377	27.127032648875726	30.253028237603534	19.299990677758355
20	24.425277525017037	27.70416415431731	29.99272739599658	17.87783092466907
21	23.676641883733126	26.134229607753344	32.591341489405444	17.597787019108086
22	23.00092061059741	23.002521399566657	32.4223483950835	21.574209594752425
23	19.106609092669675	27.331933636939176	32.27325530481062	21.288201965580527
24	19.120325656974778	26.827590947569767	34.54854141445086	19.5035419810046
25	20.29018851330334	27.84989872655668	31.775598263489236	20.08431449665074
26	19.493466426904053	29.784907322165687	29.928695837226755	20.792930413703505
27	19.095215241770923	28.75619638730179	32.19478525729858	19.95380311362871
28	17.621390809399706	28.22313366054297	34.088581383954256	20.066894146103067
29	19.0057907756262	27.01893231142314	32.93168177947469	21.043595133475982
30	20.54308178242516	27.192884712747833	32.47777963664601	19.786253868180992
31	22.53273691912155	27.757429622568488	30.318158377038536	19.39167508127143
32	23.24477413024584	27.320665338116452	30.134601241898363	19.299959289739352
33	22.105389040370955	27.549138728452515	30.14411181165682	20.201360419519705
34	19.800001820505102	27.168778714152136	32.230159554717005	20.801059910625757
35	18.97264502755711	28.154236958827394	31.891388665598008	20.981729348017485
36	22.37724067297168	27.14423328329037	30.147030897424266	20.33149514631368
37	20.041658178823194	28.93269121816588	31.039423665750242	19.98622693726069
38	20.128320499295498	28.626689420887914	29.948972497503867	21.296017582312725
39	19.803611442690656	27.268843718739493	30.16693090147332	22.760613937096526
40	21.464665408425613	26.48304466295388	30.538313942338323	21.51397598628218
41	18.93890290712693	26.4636154791899	31.059417833856312	23.538063779826857
42	20.606454192795884	27.141973345922022	29.80964108114148	22.441931380140613
43	20.671647108268893	26.914818252384155	30.346564534237896	22.06697010510906
44	20.345086158542767	28.197960469301105	29.626491990248372	21.830461381907757
45	19.191011475773628	28.654718921859214	29.37466591377272	22.77960368859444
46	20.741485450554613	28.258821838151448	29.527619730383194	21.47207298091075
47	19.839079904166102	27.455633819837168	31.256283489054525	21.449002786942206
48	20.185446693884266	27.233029989054998	31.24288080493947	21.338642512121268
49	20.765277568960265	26.093739063237127	31.37734707835611	21.7636362894465
50	19.328271282881694	26.986696815905187	31.47590545803124	22.20912644318188
51	18.996280205867738	27.50168004371723	30.174558190091496	23.327481560323534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1608.0
1	3041.0
2	4474.0
3	26553.5
4	48633.0
5	34713.5
6	20794.0
7	18900.0
8	17006.0
9	17469.5
10	17933.0
11	17978.5
12	18024.0
13	17527.0
14	17030.0
15	16525.5
16	16021.0
17	15244.5
18	14468.0
19	14492.0
20	14516.0
21	14928.0
22	15340.0
23	18416.0
24	21492.0
25	25088.0
26	32490.0
27	36296.0
28	46475.5
29	56655.0
30	64352.5
31	72050.0
32	81666.0
33	91282.0
34	102439.5
35	113597.0
36	124219.5
37	134842.0
38	145804.0
39	156766.0
40	173808.5
41	190851.0
42	217578.0
43	244305.0
44	260548.0
45	276791.0
46	280792.5
47	284794.0
48	271334.0
49	257874.0
50	238176.5
51	218479.0
52	199000.0
53	179521.0
54	163071.5
55	146622.0
56	136883.5
57	127145.0
58	116346.5
59	105548.0
60	93339.5
61	81131.0
62	70541.5
63	59952.0
64	50413.5
65	40875.0
66	32279.0
67	23683.0
68	18556.5
69	13430.0
70	10450.5
71	7471.0
72	5751.5
73	4032.0
74	3453.5
75	2161.5
76	1448.0
77	1147.5
78	847.0
79	561.5
80	276.0
81	257.5
82	239.0
83	171.5
84	104.0
85	77.0
86	50.0
87	40.0
88	30.0
89	22.5
90	15.0
91	17.0
92	19.0
93	12.5
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3185929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.23464525266709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52856189403214	17.548793420519466
2	8.692697631005407	4.039434914902205
3	3.117426646002463	2.1729690666323704
4	1.595190230789242	1.4825471649163273
5	1.0526917700244438	1.222945991846008
6	0.7269808433086121	1.0134685199856217
7	0.5651897877321471	0.9192388952870657
8	0.4582094937293207	0.851706803056396
9	0.3964418909646195	0.8290068029873514
>10	6.03599835625386	35.351285139648894
>50	1.5846126492400199	25.15478246488454
>100	0.2418839846101159	7.36793867866699
>500	0.0023317326409432633	0.38478403847709847
>1k	0.001645928923018772	0.6681933853527676
>5k	0.0	0.0
>10k+	1.3716074358489764E-4	0.9929047128368784
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31156	0.9779251201141018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09623566626877121	0.0
2	0.0	0.0	0.0	0.27172608052470726	0.0
3	0.0	0.0	0.0	0.44583542194443126	0.0
4	0.0	0.0	0.0	0.6715780546270805	0.0
5	0.0	0.0	0.0	1.2041071850628184	0.0
6	0.0	0.0	0.0	1.8550319231847288	0.0
7	3.1388019004817744E-5	0.0	0.0	2.2650222274256584	0.0
8	3.1388019004817744E-5	0.0	0.0	3.0666722328087035	0.0
9	3.1388019004817744E-5	0.0	0.0	3.4803663232922015	0.0
10	3.1388019004817744E-5	0.0	0.0	4.058816125531988	0.0
11	3.1388019004817744E-5	0.0	0.0	4.672985493399256	0.0
12	3.1388019004817744E-5	0.0	0.0	5.155011301256243	0.0
13	3.1388019004817744E-5	0.0	0.0	5.378462608551541	0.0
14	3.1388019004817744E-5	0.0	0.0	5.5046110569319024	0.0
15	3.1388019004817744E-5	0.0	0.0	5.648462348030982	0.0
16	3.1388019004817744E-5	0.0	0.0	5.897557666853216	0.0
17	3.1388019004817744E-5	0.0	0.0	6.227414358574846	0.0
18	3.1388019004817744E-5	0.0	0.0	6.615527213569417	0.0
19	3.1388019004817744E-5	0.0	0.0	6.858344928590688	0.0
20	3.1388019004817744E-5	0.0	0.0	7.120780155489968	0.0
21	3.1388019004817744E-5	0.0	0.0	7.425055611722672	0.0
22	3.1388019004817744E-5	0.0	0.0	7.780305210819199	0.0
23	3.1388019004817744E-5	0.0	0.0	8.138160015493126	0.0
24	3.1388019004817744E-5	0.0	0.0	8.41663452010387	0.0
25	3.1388019004817744E-5	0.0	0.0	8.660017219467226	0.0
26	3.1388019004817744E-5	0.0	0.0	8.892100231988849	0.0
27	3.1388019004817744E-5	0.0	0.0	9.14241968355227	0.0
28	3.1388019004817744E-5	0.0	0.0	9.408935352922178	0.0
29	3.1388019004817744E-5	0.0	0.0	9.69594739870223	0.0
30	3.1388019004817744E-5	0.0	0.0	10.042816396724472	0.0
31	3.1388019004817744E-5	0.0	0.0	10.34106535330825	0.0
32	3.1388019004817744E-5	0.0	0.0	10.625126925301851	0.0
33	3.1388019004817744E-5	0.0	0.0	10.916533293742578	0.0
34	3.1388019004817744E-5	0.0	0.0	11.220369317709215	0.0
35	3.1388019004817744E-5	0.0	0.0	11.549817965183781	0.0
36	3.1388019004817744E-5	0.0	0.0	11.848820234223675	0.0
37	3.1388019004817744E-5	0.0	0.0	12.149674396384853	0.0
38	3.1388019004817744E-5	0.0	0.0	12.488068629275794	0.0
39	3.1388019004817744E-5	0.0	0.0	12.982775196810726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14925	0.0	42.99498	1
CGACGGT	460	0.0	40.597824	28
CGGTCTA	475	0.0	39.31579	31
CGTTAGG	545	0.0	38.394497	2
TACGGGA	1620	0.0	38.333332	4
TAGGGAC	4185	0.0	38.279568	5
ATAGCGG	530	0.0	38.207542	2
TAAGGGA	5655	0.0	37.99735	4
TATAGCG	215	0.0	37.67442	1
TATGGGA	4540	0.0	37.665195	4
TAGGGAT	6275	0.0	37.57769	5
CGGGATC	1240	0.0	37.560482	6
TTAGGGA	5710	0.0	37.552536	4
AGGGCGA	2325	0.0	37.35484	6
ACGGGAT	2015	0.0	37.295288	5
GGGCGAT	4135	0.0	37.16445	7
TTACGGG	1005	0.0	37.164177	3
AGGGATC	4645	0.0	37.152855	6
GTAGGGA	5680	0.0	37.117077	4
AAGGGAT	6270	0.0	37.105263	5
>>END_MODULE