FastQCFastQC Report
Thu 26 May 2016
SRR1547949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547949_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1111984
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182551.6416603116591606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16160.14532583202636007No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.1401099296392754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.0331874E-445.01
ACGTTAC207.0331874E-445.044
GCGCGTC551.8189894E-1245.038
CGTGATA207.0331874E-445.017
GCTGCGA253.890723E-545.09
GCGATAC406.8139343E-945.09
ACGTCGA207.0331874E-445.035
TCGCGCG207.0331874E-445.01
AGTAACG207.0331874E-445.01
AGTCGGC1050.045.030
CGTTTTT67600.044.2344671
CTACGGG1300.043.269233
ATTAGCG800.042.18751
GCGCGAC1550.042.0967759
ACGGGTA650.041.538465
CGCATCG1200.041.25000421
CGACGGT4200.041.2528
TACGGGT556.184564E-1140.9090924
CGTTCGG556.184564E-1140.90909245
TAGGGCG3500.040.55