##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547949_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1111984
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271440056691464	31.0	31.0	33.0	30.0	34.0
2	31.61530561590814	31.0	31.0	34.0	30.0	34.0
3	31.55767618958546	31.0	31.0	34.0	30.0	34.0
4	35.440242845220794	37.0	35.0	37.0	33.0	37.0
5	35.37777432049382	37.0	35.0	37.0	33.0	37.0
6	35.47951679160851	37.0	35.0	37.0	33.0	37.0
7	35.88503971280162	37.0	35.0	37.0	35.0	37.0
8	35.90952657592196	37.0	35.0	37.0	35.0	37.0
9	37.69515208851926	39.0	37.0	39.0	35.0	39.0
10	37.094977985294754	39.0	37.0	39.0	33.0	39.0
11	36.79668232636441	39.0	37.0	39.0	32.0	39.0
12	36.2310671736284	38.0	35.0	39.0	32.0	39.0
13	35.993939661002315	38.0	35.0	39.0	31.0	39.0
14	36.95552004345386	39.0	35.0	41.0	31.0	41.0
15	37.205907638958834	39.0	35.0	41.0	32.0	41.0
16	37.320791486208435	39.0	35.0	41.0	32.0	41.0
17	37.2711666714629	39.0	35.0	41.0	32.0	41.0
18	37.1591398797105	39.0	35.0	41.0	32.0	41.0
19	37.04831274550713	38.0	35.0	41.0	32.0	41.0
20	36.81251079152218	38.0	35.0	41.0	32.0	41.0
21	36.690590871811104	38.0	35.0	41.0	31.0	41.0
22	36.61778047166146	38.0	35.0	40.0	31.0	41.0
23	36.51871789522151	38.0	35.0	40.0	31.0	41.0
24	36.454340170362165	38.0	35.0	40.0	31.0	41.0
25	36.42097188448755	38.0	35.0	40.0	31.0	41.0
26	36.282232478165156	38.0	35.0	40.0	31.0	41.0
27	36.19699384163801	38.0	35.0	40.0	31.0	41.0
28	36.12505575619793	38.0	35.0	40.0	30.0	41.0
29	36.10631537863854	37.0	35.0	40.0	30.0	41.0
30	35.98835414898056	37.0	35.0	40.0	30.0	41.0
31	35.79397545288421	37.0	35.0	40.0	30.0	41.0
32	35.55781108361271	37.0	34.0	40.0	29.0	41.0
33	35.33689963164937	37.0	34.0	40.0	28.0	41.0
34	35.12117620397416	37.0	34.0	40.0	27.0	41.0
35	34.96769108188607	37.0	34.0	40.0	25.0	41.0
36	34.80947028014791	37.0	34.0	40.0	24.0	41.0
37	34.660598533791855	37.0	34.0	40.0	24.0	41.0
38	34.525305220218996	37.0	34.0	40.0	24.0	41.0
39	34.46163883653002	37.0	34.0	40.0	23.0	41.0
40	34.28061644771867	36.0	33.0	40.0	23.0	41.0
41	34.18686689736543	36.0	33.0	40.0	23.0	41.0
42	34.18686150160434	36.0	33.0	40.0	23.0	41.0
43	34.12647034489705	36.0	33.0	40.0	23.0	41.0
44	33.97288450193528	36.0	33.0	40.0	22.0	41.0
45	33.86858623865091	35.0	33.0	40.0	23.0	41.0
46	33.819385890444465	35.0	33.0	40.0	22.0	41.0
47	33.742430646484124	35.0	33.0	40.0	22.0	41.0
48	33.6158901566929	35.0	33.0	40.0	21.0	41.0
49	33.49848199254666	35.0	33.0	40.0	20.0	41.0
50	33.39635552310105	35.0	33.0	40.0	20.0	41.0
51	33.10772097440251	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	4.0
14	13.0
15	33.0
16	90.0
17	281.0
18	548.0
19	1111.0
20	1825.0
21	3055.0
22	4906.0
23	7363.0
24	11294.0
25	16406.0
26	21446.0
27	23371.0
28	23842.0
29	25907.0
30	29769.0
31	35690.0
32	43700.0
33	56222.0
34	92952.0
35	137874.0
36	90953.0
37	106200.0
38	142854.0
39	234228.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.938125008992934	24.84846904272004	29.906095771162178	14.307310177124851
2	32.63176448581994	26.073666527575934	28.86165628282421	12.43291270377991
3	27.44832659462726	25.022482337875363	34.427653635304104	13.10153743219327
4	23.958977827019094	27.394458913077884	34.630084605533895	14.016478654369127
5	22.00202520899581	31.587864573590984	33.13096231600455	13.279147901408653
6	19.68715377199672	40.765784399775534	29.681811968517536	9.865249859710213
7	85.99728053641059	3.2725291011381463	9.332958028173067	1.3972323342781912
8	86.77462985078922	2.4771939164592296	8.933311990100577	1.8148642426509733
9	82.6168362134707	4.8873904660498715	10.315346263975021	2.1804270565044104
10	40.80886055914474	33.05119498122275	15.36577864429704	10.774165815335472
11	31.957564137613492	21.711373544943093	29.357976373760774	16.97308594368264
12	32.445340940157415	22.167135498352494	29.22766874343516	16.159854818054935
13	22.291597720830516	33.25911164189413	29.109951222319747	15.33933941495561
14	16.246816500956847	37.358720988791205	29.956546137354493	16.43791637289745
15	14.747154635318493	28.07378523431992	41.407879969495966	15.771180160865624
16	18.508449761867077	25.437596224406107	39.78510482165211	16.268849192074704
17	18.744694168261415	25.35369213945524	29.155995050288492	26.745618641994852
18	19.498751780601157	28.85086476064404	33.88430049353228	17.76608296522252
19	23.394581216995928	30.02165498784155	27.904897912200177	18.678865882962345
20	24.109609490784038	30.16194477618383	27.499226607577086	18.229219125455042
21	20.18841997726586	30.57804788558109	31.90927207585721	17.324260061295846
22	20.311353400768358	27.111271385199785	29.58082130678139	22.996553907250465
23	16.629016244838056	32.372228377386726	29.492420754255456	21.506334623519763
24	17.181991827220536	31.181024187398382	35.29025597490612	16.34672801047497
25	17.70610008777105	30.6157282838602	32.60739363156304	19.07077799680571
26	16.26489230060864	35.7513237600541	27.581422034849425	20.402361904487833
27	16.64853091411387	35.62973927682413	30.204751147498527	17.516978661563478
28	14.033295443099899	33.11189729348624	34.67747737377516	18.1773298896387
29	15.457686441531532	30.501697866156345	33.40209931078145	20.63851638153067
30	17.847019381573837	33.98259327472338	31.1082713420337	17.06211600166909
31	23.227672340609217	31.952438164577906	27.091936574626974	17.727952920185903
32	23.591796284838633	32.267910329645034	26.644178333501202	17.49611505201514
33	22.319026173038463	33.04103296450309	25.887692628670916	18.752248233787537
34	17.108789335098347	33.129703305083524	28.397530899725176	21.36397646009295
35	17.17803493575447	32.41314623231989	28.391955279932084	22.016863551993556
36	24.88174290277558	31.865926128433504	26.040032950114387	17.212298018676528
37	17.765633318465014	36.343778327745724	28.28574871580886	17.6048396379804
38	19.07077799680571	35.805191441603476	24.98588109181427	20.138149469776543
39	17.80223456452611	34.821184477474496	26.778982431401893	20.597598526597505
40	20.442020748499978	32.310087195499214	26.51962618167168	20.72826587432913
41	17.122818313932576	30.811864199484884	27.460556986431463	24.604760500151084
42	19.201085627131327	32.09641505633174	25.807026000374105	22.895473316162825
43	19.00737780399718	32.005046835206265	27.28267672916157	21.704898631634986
44	18.332098303572714	34.32666297356797	26.64471790961021	20.696520813249112
45	17.281543619332652	37.74676614052	23.34287183988259	21.628818400264752
46	20.59166318939841	34.938722139886906	25.521230521302464	18.948384149412224
47	19.470873681635705	33.803184218477966	26.396512899466178	20.329429200420147
48	19.11385415617491	33.065673606814485	27.7957236794774	20.0247485575332
49	20.295705693607104	30.620404610138273	27.852109382868818	21.231780313385805
50	18.04989999856113	34.39869638412063	26.816123253571995	20.73528036374624
51	17.132530683894732	35.4129196103541	25.318619692369676	22.13593001338149
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1405.0
1	1703.5
2	2002.0
3	12347.5
4	22693.0
5	15447.5
6	8202.0
7	8026.0
8	7850.0
9	9560.5
10	11271.0
11	12964.5
12	14658.0
13	15496.5
14	16335.0
15	15869.5
16	15404.0
17	14367.5
18	13331.0
19	12240.0
20	11149.0
21	10505.0
22	9861.0
23	9624.0
24	9387.0
25	9718.0
26	10637.5
27	11226.0
28	12962.0
29	14698.0
30	17499.5
31	20301.0
32	23333.5
33	26366.0
34	28015.5
35	29665.0
36	33311.5
37	36958.0
38	41756.5
39	46555.0
40	57152.0
41	67749.0
42	78406.5
43	89064.0
44	93073.5
45	97083.0
46	100097.5
47	103112.0
48	102815.0
49	102518.0
50	94460.5
51	86403.0
52	75856.0
53	65309.0
54	56584.5
55	47860.0
56	42382.5
57	36905.0
58	32383.5
59	27862.0
60	23294.0
61	18726.0
62	15440.5
63	12155.0
64	10132.5
65	8110.0
66	6207.5
67	4305.0
68	3290.5
69	2276.0
70	1875.5
71	1475.0
72	1127.5
73	780.0
74	623.5
75	315.5
76	164.0
77	119.0
78	74.0
79	69.0
80	64.0
81	57.5
82	51.0
83	52.5
84	54.0
85	41.5
86	29.0
87	18.5
88	8.0
89	9.0
90	10.0
91	6.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1111984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.957547829040962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24307268338919	24.04584094083415
2	9.140133151078578	5.84192484678793
3	3.860304898249358	3.700976352614548
4	2.202508455806727	2.815470772812425
5	1.41260776358222	2.257174008417669
6	0.9535572594067108	1.8284011035131504
7	0.7368413193132586	1.648334919305697
8	0.5671585498203742	1.4499997186027296
9	0.4785560745363692	1.3764130776786696
>10	4.584716504640197	32.745247256310115
>50	0.7435201174208179	15.663494831700575
>100	0.0716230706078062	3.6538800340143083
>500	0.003979059477719624	0.8347210278881708
>1k	0.0011368741364913212	0.48003271299118416
>5k	0.0	0.0
>10k+	2.842185341228303E-4	1.658088396528679
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18255	1.6416603116591606	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1616	0.14532583202636007	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1558	0.1401099296392754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09199772658599405	0.0
2	0.0	0.0	0.0	0.3015331155843969	0.0
3	0.0	0.0	0.0	0.4776147858242565	0.0
4	0.0	0.0	0.0	0.6985712024633448	0.0
5	0.0	0.0	0.0	1.2081109080706198	0.0
6	0.0	0.0	0.0	1.7548813651994992	0.0
7	0.0	0.0	0.0	2.1171167930473818	0.0
8	0.0	0.0	0.0	2.8780989654527405	0.0
9	0.0	0.0	0.0	3.1639843738758833	0.0
10	0.0	0.0	0.0	3.656527432049382	0.0
11	0.0	0.0	0.0	4.3862141901322325	0.0
12	0.0	0.0	0.0	4.903757608023137	0.0
13	0.0	0.0	0.0	5.102951121598872	0.0
14	0.0	0.0	0.0	5.196117929754385	0.0
15	0.0	0.0	0.0	5.319501000014388	0.0
16	0.0	0.0	0.0	5.6200448927322695	0.0
17	0.0	0.0	0.0	6.0387559533230695	0.0
18	0.0	0.0	0.0	6.5187988316378656	0.0
19	0.0	0.0	0.0	6.7789644455315905	0.0
20	0.0	0.0	0.0	7.04497546727291	0.0
21	0.0	0.0	0.0	7.40082591116419	0.0
22	0.0	0.0	0.0	7.79930286766716	0.0
23	0.0	0.0	0.0	8.23150333098318	0.0
24	0.0	0.0	0.0	8.54247902847523	0.0
25	0.0	0.0	0.0	8.811907365573605	0.0
26	0.0	0.0	0.0	9.082774572296005	0.0
27	0.0	0.0	0.0	9.364073583792573	0.0
28	0.0	0.0	0.0	9.649958992215716	0.0
29	0.0	0.0	0.0	9.985395473316164	0.0
30	0.0	0.0	0.0	10.354825249284163	0.0
31	0.0	0.0	0.0	10.691610670657132	0.0
32	0.0	0.0	0.0	10.985589720715407	0.0
33	0.0	0.0	0.0	11.280108346882688	0.0
34	0.0	0.0	0.0	11.610508784299055	0.0
35	0.0	0.0	0.0	11.999003582785363	0.0
36	0.0	0.0	0.0	12.340105613030403	0.0
37	0.0	0.0	0.0	12.682106936790458	0.0
38	0.0	0.0	0.0	13.053065511733982	0.0
39	0.0	0.0	0.0	13.563684369559274	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.0331874E-4	45.0	1
ACGTTAC	20	7.0331874E-4	45.0	44
GCGCGTC	55	1.8189894E-12	45.0	38
CGTGATA	20	7.0331874E-4	45.0	17
GCTGCGA	25	3.890723E-5	45.0	9
GCGATAC	40	6.8139343E-9	45.0	9
ACGTCGA	20	7.0331874E-4	45.0	35
TCGCGCG	20	7.0331874E-4	45.0	1
AGTAACG	20	7.0331874E-4	45.0	1
AGTCGGC	105	0.0	45.0	30
CGTTTTT	6760	0.0	44.234467	1
CTACGGG	130	0.0	43.26923	3
ATTAGCG	80	0.0	42.1875	1
GCGCGAC	155	0.0	42.096775	9
ACGGGTA	65	0.0	41.53846	5
CGCATCG	120	0.0	41.250004	21
CGACGGT	420	0.0	41.25	28
TACGGGT	55	6.184564E-11	40.909092	4
CGTTCGG	55	6.184564E-11	40.909092	45
TAGGGCG	350	0.0	40.5	5
>>END_MODULE