FastQCFastQC Report
Thu 26 May 2016
SRR1547948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547948_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2245989
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102700.45725958586618193No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43230.19247645469323313No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT25470.11340215824743577Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT302.166431E-645.0000049
ACCGTAG253.8919985E-545.025
CGGTCGA207.034726E-445.013
CGGTCTA5950.044.243731
CGACGGT6050.043.51239828
CGATACG700.041.78571310
CGCATCG1300.041.5384621
ACGACGG6350.041.45668827
TACGGGA8200.041.1585354
TCACGAC6500.040.84615325
GACGGTC6500.040.529
CGTTTTT59800.040.3720741
GCGCGAC2300.040.1086929
TCGATTG451.9297659E-840.01
ACGGTCT6600.039.88636430
CCTTACG850.039.7058831
GGCGATA7900.039.30388
TTAGGGA34500.039.1956524
GACCGAT9300.039.1935469
CTACGGG3100.039.1935463
Produced by FastQC (version 0.11.5)