FastQCFastQC Report
Thu 26 May 2016
SRR1547947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547947_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2766318
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295881.0695805760581394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA253.8922335E-545.00000439
ACGCGAG850.045.01
CGTTTTT154900.042.908331
TACGGGA14550.041.752584
CGGTCTA4600.041.57608431
GCGTAAG1950.041.538461
CGACGGT4700.041.1702128
GACGGTC4700.041.1702129
ACGGTCT4700.040.6914930
AATGCGG3850.039.740262
TATTCGA403.4605728E-739.37515
TATCCGA403.4605728E-739.37532
TCGAGCG356.2514773E-638.5714261
ACGGGAC10750.038.3023265
GCGAACG1000.038.2500041
GTTTGCG1650.038.181821
TATGGGA36250.038.172414
GGCGATA9800.038.1122448
CGAGGGA25150.038.021874
GTTACGG3200.037.968752
Produced by FastQC (version 0.11.5)