##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547947_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2766318
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44656724208858	31.0	31.0	33.0	30.0	34.0
2	31.836605552940767	31.0	31.0	34.0	30.0	34.0
3	31.819264451881526	31.0	31.0	34.0	30.0	34.0
4	35.671753211308314	37.0	35.0	37.0	33.0	37.0
5	35.6530691699219	37.0	35.0	37.0	33.0	37.0
6	35.71786938450315	37.0	35.0	37.0	33.0	37.0
7	36.0103776933816	37.0	35.0	37.0	35.0	37.0
8	36.02756660658681	37.0	35.0	37.0	35.0	37.0
9	37.81604609448372	39.0	38.0	39.0	35.0	39.0
10	37.323454498000594	39.0	37.0	39.0	34.0	39.0
11	37.072256334954986	39.0	37.0	39.0	33.0	39.0
12	36.768238141818834	39.0	35.0	39.0	33.0	39.0
13	36.689621728232254	39.0	35.0	39.0	33.0	39.0
14	37.82910786106297	40.0	36.0	41.0	33.0	41.0
15	37.9584082524135	40.0	36.0	41.0	33.0	41.0
16	37.99964826892642	40.0	36.0	41.0	33.0	41.0
17	37.9085585966617	40.0	36.0	41.0	33.0	41.0
18	37.7783190508105	39.0	36.0	41.0	33.0	41.0
19	37.687399279475464	39.0	36.0	41.0	33.0	41.0
20	37.51523975190126	39.0	35.0	41.0	33.0	41.0
21	37.40390331118837	39.0	35.0	41.0	33.0	41.0
22	37.338723892191716	39.0	35.0	41.0	32.0	41.0
23	37.199500563564996	39.0	35.0	41.0	32.0	41.0
24	37.168020813225375	39.0	35.0	41.0	32.0	41.0
25	37.19541498844312	39.0	35.0	41.0	32.0	41.0
26	37.12410178439355	39.0	35.0	41.0	32.0	41.0
27	37.05437552732549	39.0	35.0	41.0	32.0	41.0
28	37.00558287225113	39.0	35.0	41.0	32.0	41.0
29	37.00379095967998	39.0	35.0	41.0	32.0	41.0
30	36.91778747056557	39.0	35.0	41.0	31.0	41.0
31	36.77501104356043	39.0	35.0	41.0	31.0	41.0
32	36.57373772646529	39.0	35.0	41.0	30.0	41.0
33	36.38096198629369	39.0	35.0	41.0	30.0	41.0
34	36.183914864451594	39.0	35.0	41.0	30.0	41.0
35	36.03568244865558	39.0	35.0	41.0	29.0	41.0
36	35.93804797568465	39.0	35.0	41.0	29.0	41.0
37	35.85661265263068	39.0	35.0	41.0	28.0	41.0
38	35.73512083571014	39.0	35.0	41.0	27.0	41.0
39	35.667774637623005	38.0	35.0	41.0	27.0	41.0
40	35.526812535652084	38.0	35.0	40.0	26.0	41.0
41	35.414778778144814	38.0	35.0	40.0	26.0	41.0
42	35.39630476322679	38.0	35.0	40.0	26.0	41.0
43	35.336232132386804	38.0	35.0	40.0	26.0	41.0
44	35.21711603655111	38.0	34.0	40.0	25.0	41.0
45	35.1130115915813	38.0	34.0	40.0	25.0	41.0
46	35.02759805633337	38.0	34.0	40.0	24.0	41.0
47	34.974519559934905	38.0	34.0	40.0	24.0	41.0
48	34.843456175320405	38.0	34.0	40.0	24.0	41.0
49	34.710702818692575	38.0	34.0	40.0	24.0	41.0
50	34.63595906182876	37.0	34.0	40.0	24.0	41.0
51	34.36329771197671	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	3.0
13	2.0
14	15.0
15	45.0
16	150.0
17	304.0
18	731.0
19	1531.0
20	2812.0
21	4638.0
22	7580.0
23	12021.0
24	19181.0
25	30261.0
26	41608.0
27	47062.0
28	48739.0
29	51730.0
30	58938.0
31	70653.0
32	88149.0
33	115221.0
34	184504.0
35	258359.0
36	209300.0
37	276445.0
38	442712.0
39	793492.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.45682528183672	24.49176125087571	27.93594228863059	15.115471178656973
2	32.621990674969396	25.18398101736677	29.105077579656424	13.08895072800741
3	27.461665650876	25.261520909743567	33.96272590497549	13.314087534404939
4	24.253176966639412	27.274738479090253	33.390448965014144	15.081635589256187
5	22.984703855449734	31.150359430839114	31.894091713244826	13.970845000466323
6	21.87340717878422	39.33864436409697	28.378371539353033	10.409576917765781
7	87.87785786015925	2.4975436663463855	8.231157806152439	1.3934406673419326
8	88.46983607813708	1.9492697513445671	7.970269506253438	1.6106246642649182
9	83.07689137691328	5.414236541135184	9.334899313817138	2.1739727681343934
10	34.55192063963723	42.441179936652254	14.161423234783566	8.845476188926941
11	27.149300984196323	24.82100756312181	31.560543654055678	16.46914779862619
12	27.184799433759967	23.31789042329913	31.321200238005897	18.176109904935007
13	23.062677537434237	27.663160923653752	31.816804864805853	17.45735667410616
14	17.547729509044153	31.022680689638722	32.1096851482729	19.319904653044226
15	17.066439939298377	27.80634764332951	37.448623043337754	17.678589374034367
16	21.092658183187908	26.800714885273493	35.267709641480124	16.838917290058482
17	20.35879461435742	25.572909549805917	31.114897130409446	22.953398705427215
18	21.329435010725447	25.52436126287723	33.87838997541136	19.26781375098597
19	22.41466816179485	27.17572600113219	30.810340676668407	19.599265160404553
20	24.524295471453392	26.884508577828004	30.566370171469803	18.024825779248808
21	22.831395378260925	26.66540867680433	32.78462562872381	17.718570316210936
22	22.331308258848043	23.220432358102	32.468031513369034	21.980227869680927
23	18.708911990595443	28.201240782874564	32.072704584216275	21.01714264231372
24	18.63495086248219	26.89228064163267	35.62479078688712	18.847977708998027
25	19.14136408034073	28.405736433772255	32.98246983897007	19.470429646916948
26	19.687613643839935	30.156041351717338	30.35771737016496	19.798627634277764
27	18.693873950861757	29.225128853588057	32.4205677004596	19.660429495090586
28	16.837652070369348	28.86642099715217	35.00765277166255	19.28827416081593
29	17.706098864989492	26.190047565030483	35.041994448938986	21.06185912104104
30	19.18846640191041	27.032466983188485	34.393695880227796	19.38537073467331
31	21.940030032700506	27.302392566581286	31.259312920640358	19.49826448007785
32	21.559198906271803	27.19586106875638	31.899767127278935	19.34517289769289
33	20.832745909906237	27.21086295935608	31.15068477304489	20.805706357692788
34	18.583185302629705	27.93626763083637	32.380731354818934	21.099815711714996
35	18.547361510860284	28.050571192465938	32.16177605033116	21.24029124634261
36	21.490262507781104	28.39901269485287	30.19291346837204	19.91781132899399
37	19.166306982783613	29.509622537972856	30.959636599985974	20.364433879257554
38	18.817287094253082	30.35677749268161	29.81226308761321	21.0136723254521
39	19.47823785985559	29.06791626992992	29.82444534576285	21.629400524451636
40	21.685793173452943	27.965259236284478	29.42539505581065	20.923552534451932
41	18.816058023697927	27.898925575439986	30.435293411675733	22.849722989186347
42	20.373362715349426	28.32483467193577	29.532107299305434	21.769695313409375
43	20.32864623662211	27.904745586009994	29.994057082374475	21.772551094993418
44	19.716569100154068	28.63658480333787	30.154270044152554	21.492576052355513
45	19.173030721703	29.53517997569332	28.743550090770476	22.548239211833202
46	20.683124644382893	28.776301206152006	29.736819845006973	20.803754304458128
47	19.81558157811213	28.567178466105485	30.566008680130054	21.05123127565233
48	19.791253210946824	28.056391203035947	31.361759566326068	20.790596019691158
49	20.4913173395105	26.66056469285165	31.180037869832756	21.6680800978051
50	18.9899715072526	28.860962477921916	31.059661253695346	21.089404761130137
51	18.811250188879225	28.56352740357399	29.849243651669838	22.775978755876945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1552.0
1	2629.0
2	3706.0
3	24707.0
4	45708.0
5	32643.5
6	19579.0
7	18162.5
8	16746.0
9	17570.5
10	18395.0
11	18695.5
12	18996.0
13	18876.5
14	18757.0
15	18355.0
16	17953.0
17	17359.5
18	16766.0
19	16326.0
20	15886.0
21	16172.0
22	16458.0
23	17868.0
24	19278.0
25	22622.0
26	30728.0
27	35490.0
28	41189.5
29	46889.0
30	56488.0
31	66087.0
32	76070.5
33	86054.0
34	93923.0
35	101792.0
36	110818.0
37	119844.0
38	129219.5
39	138595.0
40	154564.5
41	170534.0
42	187158.5
43	203783.0
44	219725.5
45	235668.0
46	239276.0
47	242884.0
48	233706.0
49	224528.0
50	209081.0
51	193634.0
52	178344.5
53	163055.0
54	147235.0
55	131415.0
56	117413.0
57	103411.0
58	92206.0
59	81001.0
60	69927.5
61	58854.0
62	51125.0
63	43396.0
64	34907.0
65	26418.0
66	21289.5
67	16161.0
68	12569.5
69	8978.0
70	6696.0
71	4414.0
72	3674.5
73	2935.0
74	2500.0
75	1763.0
76	1461.0
77	1104.0
78	747.0
79	491.0
80	235.0
81	186.0
82	137.0
83	96.0
84	55.0
85	41.5
86	28.0
87	20.0
88	12.0
89	9.5
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2766318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.89599546856702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23095348976292	17.22487570197588
2	8.662903673000494	3.966916064833038
3	3.219479666932612	2.2113957559569823
4	1.6977539150726073	1.5548706378497734
5	1.0616073820165777	1.2153278904024427
6	0.7541164043861776	1.0359747466558784
7	0.608474476609161	0.9752140201427426
8	0.48788148846805723	0.8936425879329897
9	0.3990376891566274	0.8222728610445836
>10	5.887658621610495	34.33701251188418
>50	1.798275856559926	27.886614851084346
>100	0.18688102950864938	5.748228185765654
>500	0.0033710466203575808	0.535984055211651
>1k	0.0014447342658675346	0.5041918171427984
>5k	0.0	0.0
>10k+	1.605260295408372E-4	1.0874783121170084
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29588	1.0695805760581394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0844740192559207E-4	0.0	0.0	0.059826816728951626	0.0
2	1.0844740192559207E-4	0.0	0.0	0.17564864198548397	0.0
3	1.0844740192559207E-4	0.0	0.0	0.2951576789074864	0.0
4	1.0844740192559207E-4	0.0	0.0	0.4658177404043931	0.0
5	1.0844740192559207E-4	0.0	0.0	0.8612892660930522	0.0
6	1.0844740192559207E-4	0.0	0.0	1.3665095625304104	0.0
7	1.0844740192559207E-4	0.0	0.0	1.6637638912084582	0.0
8	1.0844740192559207E-4	0.0	0.0	2.271611579001402	0.0
9	1.0844740192559207E-4	0.0	0.0	2.5477909625719097	0.0
10	1.0844740192559207E-4	0.0	0.0	2.9711334705554457	0.0
11	1.0844740192559207E-4	0.0	0.0	3.456471743306446	0.0
12	1.0844740192559207E-4	0.0	0.0	3.8438458629846606	0.0
13	1.0844740192559207E-4	0.0	0.0	4.020145189381698	0.0
14	1.0844740192559207E-4	0.0	0.0	4.10654161958242	0.0
15	1.0844740192559207E-4	0.0	0.0	4.220013751130564	0.0
16	1.0844740192559207E-4	0.0	0.0	4.422557348793595	0.0
17	1.0844740192559207E-4	0.0	0.0	4.691073115961361	0.0
18	1.0844740192559207E-4	0.0	0.0	5.010559162034155	0.0
19	1.0844740192559207E-4	0.0	0.0	5.220947121769804	0.0
20	1.0844740192559207E-4	0.0	0.0	5.431913467649055	0.0
21	1.0844740192559207E-4	0.0	0.0	5.692476425342278	0.0
22	1.0844740192559207E-4	0.0	0.0	5.990236841895979	0.0
23	1.0844740192559207E-4	0.0	0.0	6.304698158346221	0.0
24	1.0844740192559207E-4	0.0	0.0	6.544294618333828	0.0
25	1.0844740192559207E-4	0.0	0.0	6.758767430208675	0.0
26	1.0844740192559207E-4	0.0	0.0	6.961383326139655	0.0
27	1.0844740192559207E-4	0.0	0.0	7.175241602736923	0.0
28	1.0844740192559207E-4	0.0	0.0	7.406885253249988	0.0
29	1.0844740192559207E-4	0.0	0.0	7.664050192349542	0.0
30	1.0844740192559207E-4	0.0	0.0	7.991995135772532	0.0
31	1.0844740192559207E-4	0.0	0.0	8.26448730767757	0.0
32	1.0844740192559207E-4	0.0	0.0	8.534268294534467	0.0
33	1.0844740192559207E-4	0.0	0.0	8.793529883404583	0.0
34	1.0844740192559207E-4	0.0	0.0	9.062985528055705	0.0
35	1.0844740192559207E-4	0.0	0.0	9.372024474409667	0.0
36	1.0844740192559207E-4	0.0	0.0	9.648782244123778	0.0
37	1.0844740192559207E-4	0.0	0.0	9.93327592850858	0.0
38	1.0844740192559207E-4	0.0	0.0	10.23233771388539	0.0
39	1.0844740192559207E-4	0.0	0.0	10.593359114895684	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCA	25	3.8922335E-5	45.000004	39
ACGCGAG	85	0.0	45.0	1
CGTTTTT	15490	0.0	42.90833	1
TACGGGA	1455	0.0	41.75258	4
CGGTCTA	460	0.0	41.576084	31
GCGTAAG	195	0.0	41.53846	1
CGACGGT	470	0.0	41.17021	28
GACGGTC	470	0.0	41.17021	29
ACGGTCT	470	0.0	40.69149	30
AATGCGG	385	0.0	39.74026	2
TATTCGA	40	3.4605728E-7	39.375	15
TATCCGA	40	3.4605728E-7	39.375	32
TCGAGCG	35	6.2514773E-6	38.571426	1
ACGGGAC	1075	0.0	38.302326	5
GCGAACG	100	0.0	38.250004	1
GTTTGCG	165	0.0	38.18182	1
TATGGGA	3625	0.0	38.17241	4
GGCGATA	980	0.0	38.112244	8
CGAGGGA	2515	0.0	38.02187	4
GTTACGG	320	0.0	37.96875	2
>>END_MODULE