FastQCFastQC Report
Thu 26 May 2016
SRR1547946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547946_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5730087
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT584731.0204557103583245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC351.2130658E-745.042
CCGCGTA207.035644E-445.012
CGTTTTT284650.042.9527471
CGACGGT8500.039.44117728
TACGGGA28900.039.3944634
CGGTCTA8400.039.37500431
CGAGGGC8650.039.0173384
TAGGGCG14600.038.9897275
CGAGGGA52150.038.7440074
TTAGGGA99200.038.739924
CGTTAGG7750.038.6129042
GACCGAT25900.038.5714269
TAACGGG16700.038.2634743
GGGCGAT79950.038.245787
AGGGCGA39600.038.181826
TAAGGGA88750.038.0788764
TAGGGAT104850.038.0257535
ACGGGAT31250.037.9445
TCGATAG3400.037.720591
AAGGGAT112150.037.6571545
Produced by FastQC (version 0.11.5)