##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547946_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5730087
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40027053690459	31.0	31.0	33.0	30.0	34.0
2	31.794630517826345	31.0	31.0	34.0	30.0	34.0
3	31.79415600496118	31.0	31.0	34.0	30.0	34.0
4	35.62459156379301	37.0	35.0	37.0	33.0	37.0
5	35.56685177729413	37.0	35.0	37.0	33.0	37.0
6	35.643274700715715	37.0	35.0	37.0	33.0	37.0
7	35.97977412908391	37.0	35.0	37.0	35.0	37.0
8	35.99758153759271	37.0	35.0	37.0	35.0	37.0
9	37.76751853854924	39.0	38.0	39.0	35.0	39.0
10	37.23693741473733	39.0	37.0	39.0	34.0	39.0
11	37.00608123401966	39.0	37.0	39.0	33.0	39.0
12	36.64798527491816	39.0	35.0	39.0	32.0	39.0
13	36.5507521962581	39.0	35.0	39.0	32.0	39.0
14	37.67988845544579	40.0	36.0	41.0	33.0	41.0
15	37.811849279077265	40.0	36.0	41.0	33.0	41.0
16	37.85706970941279	40.0	36.0	41.0	33.0	41.0
17	37.77770005935337	40.0	36.0	41.0	33.0	41.0
18	37.642840675892	39.0	36.0	41.0	33.0	41.0
19	37.56476629412433	39.0	36.0	41.0	33.0	41.0
20	37.401704022993016	39.0	35.0	41.0	32.0	41.0
21	37.290419150704	39.0	35.0	41.0	32.0	41.0
22	37.20823645435052	39.0	35.0	41.0	32.0	41.0
23	37.06119697659041	39.0	35.0	41.0	32.0	41.0
24	37.02575912023674	39.0	35.0	41.0	32.0	41.0
25	37.05016799919443	39.0	35.0	41.0	32.0	41.0
26	36.95264068416413	39.0	35.0	41.0	32.0	41.0
27	36.88737500844228	39.0	35.0	41.0	31.0	41.0
28	36.826909259841955	39.0	35.0	41.0	31.0	41.0
29	36.81315397130969	39.0	35.0	41.0	31.0	41.0
30	36.727948458723226	38.0	35.0	41.0	31.0	41.0
31	36.596382393495944	38.0	35.0	41.0	31.0	41.0
32	36.39494060037832	38.0	35.0	41.0	30.0	41.0
33	36.21792409085586	38.0	35.0	41.0	30.0	41.0
34	36.03019954147293	38.0	35.0	41.0	29.0	41.0
35	35.900892953283254	38.0	35.0	41.0	29.0	41.0
36	35.80550923572365	38.0	35.0	41.0	28.0	41.0
37	35.7089798113013	38.0	35.0	41.0	27.0	41.0
38	35.60892984696393	38.0	35.0	41.0	27.0	41.0
39	35.534641097072345	38.0	35.0	40.0	27.0	41.0
40	35.39947962395684	38.0	35.0	40.0	26.0	41.0
41	35.31253783755814	38.0	34.0	40.0	26.0	41.0
42	35.29742201121903	38.0	34.0	40.0	26.0	41.0
43	35.242812194649055	38.0	34.0	40.0	26.0	41.0
44	35.07400219228783	38.0	34.0	40.0	25.0	41.0
45	34.956394728387195	38.0	34.0	40.0	24.0	41.0
46	34.89245398891849	38.0	34.0	40.0	24.0	41.0
47	34.84566168018042	38.0	34.0	40.0	24.0	41.0
48	34.72954250083812	38.0	34.0	40.0	24.0	41.0
49	34.60020956051802	37.0	34.0	40.0	24.0	41.0
50	34.53538523935151	37.0	34.0	40.0	24.0	41.0
51	34.23478247363435	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	7.0
11	7.0
12	2.0
13	20.0
14	65.0
15	110.0
16	350.0
17	755.0
18	1804.0
19	3635.0
20	6640.0
21	11095.0
22	17447.0
23	27387.0
24	42536.0
25	65204.0
26	87726.0
27	98172.0
28	102857.0
29	112044.0
30	129138.0
31	155458.0
32	191584.0
33	248911.0
34	394206.0
35	557546.0
36	446173.0
37	558148.0
38	875736.0
39	1595038.0
40	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.80610940113126	25.83533199408665	30.12154963790253	14.23700896687956
2	32.637724348687904	26.396091368246243	28.037916352753456	12.928267930312401
3	27.73835720120829	24.987631077852747	33.46114989179048	13.812861829148492
4	25.227732144381054	27.233007107920002	32.740637271301466	14.798623476397479
5	23.290187391570143	31.462279717567988	31.234394870444376	14.01313802041749
6	21.778918889015124	39.96195869277378	27.96343231786882	10.29569010034228
7	87.72081471014315	2.9465870238968446	7.9398968985985725	1.392701367361438
8	88.28644312032262	2.4119529075212993	7.703303632213612	1.5983003399424827
9	83.1261898815847	5.7134734603506026	8.934593837754994	2.225742820309709
10	34.64160317286631	41.76889460840647	14.24062147747495	9.34888074125227
11	29.150552164391218	25.088135660069383	29.571191501978937	16.190120673560454
12	28.5184500689082	23.22938552241877	30.15828555482665	18.093878853846373
13	23.114465801304586	29.609114137359523	30.287829835742457	16.988590225593434
14	17.740707950856592	32.33561724280975	31.419592756619576	18.50408204971408
15	16.721264441534657	28.299762289822127	37.86204293233244	17.116930336310777
16	20.76671785262597	27.203339146508597	35.46618053094133	16.563762469924104
17	20.44876456500573	26.32668579028556	30.605486443748585	22.619063200960124
18	21.24416261044553	26.719541954598597	33.18399877698192	18.852296657973955
19	23.043856053145443	27.702354257448448	30.117727706403063	19.13606198300305
20	24.58978371532579	27.86596433876135	29.83230446588333	17.711947480029536
21	22.560477703043603	28.08278478145271	31.855258044075075	17.50147947142862
22	23.04205852371875	24.87442162745522	30.8182929857784	21.26522686304763
23	19.04946643916576	28.947378983949108	31.36912231873617	20.63403225814896
24	18.640572123948555	27.717100979444115	35.20033814495312	18.441988751654208
25	19.014231372054212	29.639392909741165	32.28247668839932	19.063899029805306
26	18.831616343696002	31.828382361384737	29.786999743633906	19.553001551285348
27	18.461517250959712	31.313311647798713	31.514216101779958	18.710954999461613
28	16.99593740897826	30.16277065252238	34.27750747938033	18.56378445911903
29	18.158572461465244	27.87713345364564	33.65388693051257	20.310407154376538
30	19.088558341260786	29.895217995817514	32.21633458619389	18.799889076727805
31	22.73199342348554	29.809250714692464	29.120919106463827	18.337836755358165
32	22.622797873749562	29.309904020654486	30.074185610096322	17.993112495499634
33	21.815584998971218	29.84057659159451	28.90675132855749	19.437087080876783
34	19.61615940560763	29.853525784163487	30.58494574340669	19.945369066822195
35	19.40900722798799	29.86664949415253	30.622781120077235	20.101562157782247
36	22.630127605392378	29.57506578870443	29.14313517403837	18.651671431864823
37	19.803817987405775	31.007923614423305	29.977747283767243	19.210511114403673
38	19.930168599534355	31.37861606638782	28.410790272468812	20.280425061609012
39	19.997933713746406	30.03413386219092	29.549376824470553	20.418555599592118
40	21.42735354628996	29.07886040822766	29.028372518602247	20.465413526880134
41	18.218763519646387	29.087132533938835	29.99537354319402	22.698730403220754
42	20.321680979712873	29.297775059959825	28.63101380485148	21.749530155475824
43	20.376130414773808	29.155979656155306	29.249852576409396	21.218037352661486
44	19.970743899699954	30.41892732169686	28.77027870606502	20.840050072538165
45	19.581971443016485	32.05426025817758	27.31246488927655	21.051303409529385
46	21.11517678527394	31.101081013255122	28.103814130570793	19.679928070900147
47	20.01194746257779	29.887591584560585	29.47943024250766	20.62103071035396
48	20.35379218500522	29.204059205383793	30.283606514176835	20.158542095434154
49	20.80019029379484	28.38953405070464	30.237813143151232	20.572462512349286
50	19.301295076322575	30.414651644905216	29.689060567492255	20.59499271127995
51	18.904826401414148	30.18243876576394	28.750732755017506	22.162002077804406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3449.0
1	5812.5
2	8176.0
3	48417.0
4	88658.0
5	63787.5
6	38917.0
7	36969.5
8	35022.0
9	37690.0
10	40358.0
11	43380.0
12	46402.0
13	47034.0
14	47666.0
15	44766.0
16	41866.0
17	38876.0
18	35886.0
19	34918.5
20	33951.0
21	35096.5
22	36242.0
23	39024.0
24	41806.0
25	47651.0
26	64140.5
27	74785.0
28	91106.0
29	107427.0
30	125489.0
31	143551.0
32	164063.0
33	184575.0
34	206081.5
35	227588.0
36	241626.0
37	255664.0
38	271868.0
39	288072.0
40	327805.0
41	367538.0
42	400232.0
43	432926.0
44	440399.5
45	447873.0
46	469704.5
47	491536.0
48	469668.5
49	447801.0
50	424216.5
51	400632.0
52	369575.0
53	338518.0
54	302906.0
55	267294.0
56	237672.0
57	208050.0
58	185989.0
59	163928.0
60	142174.5
61	120421.0
62	101114.5
63	81808.0
64	68256.5
65	54705.0
66	43084.5
67	31464.0
68	24602.5
69	17741.0
70	13755.0
71	9769.0
72	8107.0
73	6445.0
74	4905.5
75	3117.5
76	2869.0
77	1936.5
78	1004.0
79	688.0
80	372.0
81	320.0
82	268.0
83	179.0
84	90.0
85	67.0
86	44.0
87	37.0
88	30.0
89	23.5
90	17.0
91	14.0
92	11.0
93	9.0
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5730087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.68467226603524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67481723315903	14.13959158793692
2	9.616642843469627	3.5936763965948644
3	3.4970067629983252	1.960212758361974
4	1.8419511921746072	1.3766501742326172
5	1.1360078424862414	1.0612967114250602
6	0.8210073332748736	0.9204151770151551
7	0.5588755081995109	0.7309683995755226
8	0.46445941354160797	0.69426175383199
9	0.3449901965706055	0.5801425882125154
>10	3.2083989653349514	13.988035247783042
>50	1.453377685280559	19.95753445386391
>100	1.3714982603441033	38.06562437245535
>500	0.008678047375433583	1.073142364316579
>1k	0.0020979894745442626	0.7191106899801566
>5k	9.53631579338301E-5	0.09744824727443074
>10k+	9.53631579338301E-5	1.0418890771398408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58473	1.0204557103583245	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.490348401341899E-5	0.0	0.0	0.0349209357554257	0.0
2	3.490348401341899E-5	0.0	0.0	0.15978814981343215	0.0
3	3.490348401341899E-5	0.0	0.0	0.26910586174346046	0.0
4	3.490348401341899E-5	0.0	0.0	0.42091856545982637	0.0
5	3.490348401341899E-5	0.0	0.0	0.789796036255645	0.0
6	3.490348401341899E-5	0.0	0.0	1.217293908452001	0.0
7	3.490348401341899E-5	0.0	0.0	1.4962949079132655	0.0
8	3.490348401341899E-5	0.0	0.0	2.0054145774750016	0.0
9	3.490348401341899E-5	0.0	0.0	2.2217638231321795	0.0
10	3.490348401341899E-5	0.0	0.0	2.5843761185475893	0.0
11	3.490348401341899E-5	0.0	0.0	3.0675974029713684	0.0
12	3.490348401341899E-5	0.0	0.0	3.407330464615982	0.0
13	3.490348401341899E-5	0.0	0.0	3.548113667384108	0.0
14	3.490348401341899E-5	0.0	0.0	3.6022489710889207	0.0
15	3.490348401341899E-5	0.0	0.0	3.688320962666012	0.0
16	3.490348401341899E-5	0.0	0.0	3.8999931414653912	0.0
17	3.490348401341899E-5	0.0	0.0	4.177126804531938	0.0
18	3.490348401341899E-5	0.0	0.0	4.5312051981060675	0.0
19	3.490348401341899E-5	0.0	0.0	4.709753970576712	0.0
20	3.490348401341899E-5	0.0	0.0	4.8961385752083695	0.0
21	3.490348401341899E-5	0.0	0.0	5.139223889619826	0.0
22	3.490348401341899E-5	0.0	0.0	5.424315547041433	0.0
23	3.490348401341899E-5	0.0	0.0	5.71523608629328	0.0
24	3.490348401341899E-5	0.0	0.0	5.9280600800651015	0.0
25	3.490348401341899E-5	0.0	0.0	6.109942135259028	0.0
26	3.490348401341899E-5	0.0	0.0	6.295646121952424	0.0
27	3.490348401341899E-5	0.0	0.0	6.493269648436402	0.0
28	3.490348401341899E-5	0.0	0.0	6.70267310077491	0.0
29	3.490348401341899E-5	0.0	0.0	6.940138954260206	0.0
30	3.490348401341899E-5	0.0	0.0	7.22954119195747	0.0
31	3.490348401341899E-5	0.0	0.0	7.488594850305065	0.0
32	3.490348401341899E-5	0.0	0.0	7.722744872809087	0.0
33	3.490348401341899E-5	0.0	0.0	7.966650419094859	0.0
34	3.490348401341899E-5	0.0	0.0	8.22158546632887	0.0
35	3.490348401341899E-5	0.0	0.0	8.519486702383402	0.0
36	3.490348401341899E-5	0.0	0.0	8.780302986673675	0.0
37	3.490348401341899E-5	0.0	0.0	9.043527611360874	0.0
38	3.490348401341899E-5	0.0	0.0	9.328898496654588	0.0
39	3.490348401341899E-5	0.0	0.0	9.633605912091737	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGC	35	1.2130658E-7	45.0	42
CCGCGTA	20	7.035644E-4	45.0	12
CGTTTTT	28465	0.0	42.952747	1
CGACGGT	850	0.0	39.441177	28
TACGGGA	2890	0.0	39.394463	4
CGGTCTA	840	0.0	39.375004	31
CGAGGGC	865	0.0	39.017338	4
TAGGGCG	1460	0.0	38.989727	5
CGAGGGA	5215	0.0	38.744007	4
TTAGGGA	9920	0.0	38.73992	4
CGTTAGG	775	0.0	38.612904	2
GACCGAT	2590	0.0	38.571426	9
TAACGGG	1670	0.0	38.263474	3
GGGCGAT	7995	0.0	38.24578	7
AGGGCGA	3960	0.0	38.18182	6
TAAGGGA	8875	0.0	38.078876	4
TAGGGAT	10485	0.0	38.025753	5
ACGGGAT	3125	0.0	37.944	5
TCGATAG	340	0.0	37.72059	1
AAGGGAT	11215	0.0	37.657154	5
>>END_MODULE