FastQCFastQC Report
Thu 26 May 2016
SRR1547945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547945_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3671365
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344780.9391057549440059No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42910.1168775101358759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA302.1668093E-645.00000423
TACGTCG207.0353114E-445.02
CGTTTTT145850.042.840251
CGTAAGG6200.041.733872
CGACGGT4650.041.61290428
GCGTAAG2500.041.3999981
ACGGGAT20500.041.2682955
CGTCGGT603.6379788E-1241.25000412
TAGGGCG7850.040.127395
TATAGCG3200.040.0781251
AATCGCG900.040.0000041
CGGTCTA4800.039.84375431
TTCGCGG2350.039.2553182
TACGGGA18450.039.1463434
GACCGAT15200.038.7828949
GGCGATA11500.038.7391328
GGGCGAT46450.038.6544657
CGGGATC15050.038.5714266
TCACGAC5500.038.45454425
CTATGCG1700.038.382351
Produced by FastQC (version 0.11.5)